Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	92.9%	175.8 / 6.6	56	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (23/29);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

AATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGA  >  NC_000913/4606076‑4606331                                                                                                                                       |                                                                                                                               aaTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑ggggg‑ggg                                                                                                                       >  1:382780/1‑140 (MQ=255)  aTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑ggggg‑ggg                                                                                                                       <  1:128351/140‑1 (MQ=255)      gTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑ggggg‑ggggactt                                                                                                                  <  2:1095636/140‑6 (MQ=255)         cGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGAGCGTCTACCAATACCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGaa                                                                                                               <  2:966545/140‑1 (MQ=255)              gggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAAcccc                                                                                                           <  1:612733/140‑1 (MQ=255)                gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGG‑GGGA‑CTTGAACCCCCAc                                                                                                        <  2:1126774/140‑1 (MQ=255)                 ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCTCCTCGATTCATGCG‑A‑GGCGG‑CGGACCTTGAACCCCCAc                                                                                                        >  2:475807/1‑140 (MQ=18)                  gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACg                                                                                                       <  2:343786/140‑1 (MQ=255)                    gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTc                                                                                                     <  1:782084/140‑1 (MQ=255)                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCg                                                                                                   >  1:236155/1‑140 (MQ=255)                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTAAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTc                                                                                                     <  1:169605/138‑1 (MQ=255)                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTAAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTc                                                                                                     >  2:169605/1‑138 (MQ=255)                       ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGG‑CTTGAACCCCCACGTCCGt                                                                                                  <  1:156262/140‑1 (MQ=255)                       ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGt                                                                                                  >  1:818701/1‑140 (MQ=255)                           aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGa                                                                                              >  1:159789/1‑140 (MQ=255)                           aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGa                                                                                              >  1:405206/1‑140 (MQ=255)                                cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACt                                                                                         <  2:382780/140‑1 (MQ=255)                                 aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTa                                                                                        >  2:1016089/1‑140 (MQ=255)                                 aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTa                                                                                        >  2:948397/1‑140 (MQ=255)                                  cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                       <  2:405206/140‑1 (MQ=255)                                  cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                       <  1:818815/140‑1 (MQ=255)                                  cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                       <  1:450049/140‑1 (MQ=255)                                              cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                           <  1:948397/140‑1 (MQ=255)                                              cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                           <  1:1016089/140‑1 (MQ=255)                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGC‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                          >  2:179905/1‑140 (MQ=255)                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGC‑CTTGAACCCCAACGTCCGTAAGACCACTAACACCTCAACCTAg                                                                          >  2:279890/1‑140 (MQ=37)                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                          <  1:477193/140‑1 (MQ=255)                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                          <  1:315072/140‑1 (MQ=255)                                               acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                          <  1:348885/140‑1 (MQ=255)                                                 tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                        >  1:256752/1‑140 (MQ=255)                                                 tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTAGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                        <  2:256752/140‑1 (MQ=255)                                                   gcacCTGACGCTAGAGAGTCTACCAGTTCCGGCACCTACGCAAAGTCGTCTTACCATTTTTGAAAGCACGTAGATTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                      <  2:272145/139‑1 (MQ=12)                                                   acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                      <  1:279890/140‑1 (MQ=255)                                                   acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                      <  2:236155/140‑1 (MQ=255)                                                      ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                   >  1:419192/1‑140 (MQ=255)                                                       cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGC‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                  >  1:616798/1‑140 (MQ=255)                                                         gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                >  1:271078/1‑140 (MQ=255)                                                            gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                             <  2:271078/140‑1 (MQ=255)                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                            >  1:570578/1‑140 (MQ=255)                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGCTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                            >  2:287812/1‑140 (MQ=255)                                                               aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                           <  1:802056/123‑1 (MQ=255)                                                               aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                           >  2:802056/1‑123 (MQ=255)                                                                 cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACTCCCCCTCACTAAATAACAATAAACCCTGAAGCAAGCGCGTCTACCAATTcccc                                                        >  2:613037/1‑138 (MQ=25)                                                                 cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                        <  1:287812/140‑1 (MQ=255)                                                                 cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                        <  2:419192/140‑1 (MQ=255)                                                                 cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                        >  1:487559/1‑140 (MQ=255)                                                                 cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTATTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                       >  2:257720/1‑140 (MQ=255)                                                                           aaTTCCGCCACCGTCACACAGTCAACTAACTATTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              <  1:737410/140‑1 (MQ=18)                                                                               ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGGCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                          <  2:818701/140‑1 (MQ=255)                                                                               ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              <  2:505275/136‑1 (MQ=255)                                                                               ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              >  1:505275/1‑136 (MQ=255)                                                                                cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              >  1:114827/1‑135 (MQ=255)                                                                                cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              <  2:114827/135‑1 (MQ=255)                                                                                cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              >  1:956938/1‑135 (MQ=255)                                                                                cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                         <  1:179905/140‑1 (MQ=255)                                                                                cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                         >  1:566107/1‑140 (MQ=255)                                                                                cGCCACCTTCGCACAGTCATCTTACTTTTGTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGGTGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                              <  2:956938/135‑1 (MQ=255)                                                                                  ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGTTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                       <  2:159789/140‑1 (MQ=255)                                                                                  ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                        >  2:1112054/1‑123 (MQ=255)                                                                                  ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                        <  1:1112054/123‑1 (MQ=255)                                                                                  ccACCTTCGCACAGTAATCTTACTTTTTTTGATATCGCATCGGTTAGGGCG‑AGGGGGG‑GGGA‑TTTGAACCCCAATGGCCGTAAGAACCATAACCACAGAAGCTAGCGCGGCTACCATTTCCCCCACTTTCCCCCattacg                                       >  2:502258/1‑135 (MQ=255)                                                                                    aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                     <  1:475807/140‑1 (MQ=255)                                                                                                tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTTTACCAATTCCGCCACCTTCCCCCAGTGCGAGCAATATCAACGt                         >  2:1078777/1‑140 (MQ=255)                                                                                                             tttGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                <  2:79639/136‑1 (MQ=255)                                                                                                             tttGATATCGCCTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                >  1:79639/1‑136 (MQ=255)                                                                                                             tttGATATCGCCTCGTTTGGTGCG‑A‑GGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCgt           <  1:257720/140‑2 (MQ=255)                                                                                                                   atCGCCTCGTTTGGTGGGTC‑GGGGG‑GGGA‑CTTGAACCCCCACAAGCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg      <  2:616798/140‑1 (MQ=21)                                                                                                                       ccTCGTTTGGTGCG‑AGGGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa  >  1:470429/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                               AATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑GGGGG‑GGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGA  >  NC_000913/4606076‑4606331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑