Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,382	(C)6→7	coding (407/555 nt)	yfcE ←	phosphodiesterase YfcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,376	1	.	C	100.0%	206.7 / NA	55	E138G (GAG→GGG)	yfcE	phosphodiesterase YfcE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/30);  total (25/30)

ATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑CCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACC  >  NC_000913/2419244‑2419510                                                                                                                                      |                                                                                                                                       aTCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑ccccccc                                                                                                                                 >  2:569311/1‑139 (MQ=255) aTCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑ccc                                                                                                                                     >  1:708146/1‑136 (MQ=255) aTCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑ccc                                                                                                                                     <  2:708146/136‑1 (MQ=255)           gcgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGc                                                                                                                        <  2:982974/140‑1 (MQ=255)                aTGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTc                                                                                                                           <  2:615765/132‑1 (MQ=255)                aTGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTc                                                                                                                           >  1:615765/1‑132 (MQ=255)                    tACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAg                                                                                                               <  2:651830/140‑1 (MQ=255)                           aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTa                                                                                                        >  1:180881/1‑140 (MQ=255)                           aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTa                                                                                                        >  1:1165496/1‑140 (MQ=255)                           aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGG‑AAATCTCCCCCCCGCTGTTCTGCCACTGGCAg                                                                                                               <  2:428808/132‑1 (MQ=255)                           aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGG‑AAATCTCCCCCCCGCTGTTCTGCCACTGGCAg                                                                                                               >  1:428808/1‑132 (MQ=255)                            tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTAt                                                                                                       <  1:1084877/140‑1 (MQ=255)                            tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTAt                                                                                                       <  1:563011/140‑1 (MQ=255)                              aTTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGa                                                                                                     <  2:749483/140‑1 (MQ=255)                               ttGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGAc                                                                                                    >  2:350380/1‑140 (MQ=255)                               ttGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGAc                                                                                                    <  2:180881/140‑1 (MQ=255)                                 gAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCg                                                                                                  <  2:1165496/140‑1 (MQ=255)                                  aGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCg                                                                                                  >  1:80688/1‑139 (MQ=255)                                  aGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCg                                                                                                  <  2:80688/139‑1 (MQ=255)                                         ttACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAg                                                                                          <  1:1067109/140‑1 (MQ=255)                                           acacTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGca                                                                                        <  1:407695/140‑1 (MQ=255)                                                      gTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTgg                                                                             >  1:991799/1‑140 (MQ=255)                                                       tCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt                                                                            >  2:398250/1‑140 (MQ=255)                                                             tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc                                                                                >  2:56875/1‑130 (MQ=255)                                                             tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc                                                                                <  1:56875/130‑1 (MQ=255)                                                             tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAg                                                                      <  2:979652/140‑1 (MQ=255)                                                             tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAg                                                                      >  2:375238/1‑140 (MQ=255)                                                             tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAg                                                                      >  2:1193148/1‑140 (MQ=255)                                                                  cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggc                                                                 >  2:349418/1‑140 (MQ=255)                                                                  cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggc                                                                 >  1:49357/1‑140 (MQ=255)                                                                  cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggc                                                                 >  2:90266/1‑140 (MQ=255)                                                                  cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggc                                                                 >  2:900499/1‑140 (MQ=255)                                                                     gCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGa                                                              >  1:24889/1‑140 (MQ=255)                                                                       cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtt                                                            >  1:1150210/1‑140 (MQ=255)                                                                            cTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCg                                                       <  1:569311/140‑1 (MQ=255)                                                                               gCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGc                                                    >  1:569871/1‑140 (MQ=255)                                                                                ccGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGcc                                                   <  1:246077/140‑1 (MQ=255)                                                                                 cGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCa                                                  <  2:1150210/140‑1 (MQ=255)                                                                                           ccTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGAc                                        >  2:474634/1‑140 (MQ=255)                                                                                              ttCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCAt                                     <  1:1193148/140‑1 (MQ=255)                                                                                              ttCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCAt                                     <  2:601062/140‑1 (MQ=255)                                                                                                   aaTACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTc                                <  2:49357/140‑1 (MQ=255)                                                                                                        tCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCaaaaa                           >  2:910395/1‑140 (MQ=255)                                                                                                        tCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCaaaaa                           >  1:1071164/1‑140 (MQ=255)                                                                                                          aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                         >  2:675856/1‑140 (MQ=255)                                                                                                          aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                         >  2:859070/1‑140 (MQ=255)                                                                                                          aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                         >  2:952687/1‑140 (MQ=255)                                                                                                           ccGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAg                        <  2:430775/140‑1 (MQ=255)                                                                                                           ccGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAg                        <  2:724839/140‑1 (MQ=255)                                                                                                               gCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgt                    >  1:1118141/1‑140 (MQ=255)                                                                                                                     gTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGtttt              <  1:349418/140‑1 (MQ=255)                                                                                                                     gTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGtttt              <  2:24889/140‑1 (MQ=255)                                                                                                                     gTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGtttt              <  1:910395/140‑1 (MQ=255)                                                                                                                      ttGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGttttt             <  2:1071164/140‑1 (MQ=255)                                                                                                                        gAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTcc           <  1:375238/140‑1 (MQ=255)                                                                                                                             ggAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTaa      <  1:350380/140‑1 (MQ=255)                                                                                                                               aaaaTCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATa    <  1:759286/140‑1 (MQ=255)                                                                                                                                 aaTCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATAcc  <  1:90266/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑CCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACC  >  NC_000913/2419244‑2419510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑