Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,834	A→G	T340A (ACT→GCT)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,834	0	A	G	100.0%	130.2 / NA	35	T340A (ACT→GCT)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/23);  total (12/23)

TGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGA  >  NC_000913/31704‑31969                                                                                                                                   |                                                                                                                                         tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGAc                                                                                                                                 <  2:1097382/140‑1 (MQ=255)    tATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGTACACCCCCGACGAACTGATGAAAGACATCGCTggcgggcgga                                                                                                                              >  2:342003/1‑138 (MQ=255)      tCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTACACCCCCGACCAACTGATGAAAGACATCGCTGGGGGGCGGAcc                                                                                                                            >  2:555170/1‑139 (MQ=255)           aTGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGc                                                                                                                       >  1:623030/1‑140 (MQ=255)                    cgcgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGGCGGACTCCGGCCTCCTTcca                                                                                                              >  2:119479/1‑138 (MQ=255)                    cgcgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGTACACCCTCGACGAACTGATGAAAGACATCGCTGGCGGGCGGACTCCCGCCTCCTTccg                                                                                                              >  2:661218/1‑138 (MQ=255)                      cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  1:431979/140‑1 (MQ=255)                      cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  1:804566/140‑1 (MQ=255)                      cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  1:661218/140‑1 (MQ=255)                      cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  1:40698/140‑1 (MQ=255)                      cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  1:342003/140‑1 (MQ=255)                      cgTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  2:623030/140‑1 (MQ=255)                      caggtCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGc                                                                                                           <  1:468443/137‑1 (MQ=255)                        tgtCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTTCACCCTCGACGAACTGATGAACGACATCGCCGGCGGGCGTACTCCCGCCCCCCTC‑GAGccc                                                                                                         >  2:921522/1‑139 (MQ=255)                              gTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGGACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATc                                                                                                   <  1:422852/140‑1 (MQ=255)                                tcttcGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGa                                                                                                 <  1:555170/140‑1 (MQ=255)                                 cttcGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGAc                                                                                                <  2:1050043/140‑1 (MQ=255)                                    cGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTACACCCCCGACGAAACGATGAAAGACATCGCCGGCGGACGGACTCCGGCCCCCTTC‑GAGCCCGCCATCGAcctg                                                                                             >  2:468443/1‑137 (MQ=255)                                                    aGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTc                                                                             <  1:872274/140‑1 (MQ=255)                                                    aGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTc                                                                             <  1:629700/140‑1 (MQ=255)                                                                             gTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGc                                                    <  1:119479/140‑1 (MQ=255)                                                                               ggcggcGAAACTGGCGGTGGGTTACACCCCCGACGAAATGATGAACGACATCGCTGGCGGGCGTAATCCCGCCCCCTTC‑GAGCCGTCCCTCGACCATGTGGGTACTAAAAATTCTCGCTTTAACTTCGAAAAAATTGCCg                                                  >  2:613947/1‑140 (MQ=255)                                                                               ggcggcGAAACTGGCGGTGGGGTACACCCTCGACGAAATGATGAACGACATCGCTGGCGGGCGTACTCCCGCCCCCTTC‑CAGCCGTCCATCGACTATGTGGTTACTAAAAATTCTCCCTTCAACTTCGAAAAAATTGccc                                                  >  2:1018470/1‑139 (MQ=255)                                                                                   gcgAAACTGGCGGGGGGGTACACCCTCGACGAAATGATGAACGACATCGCTGGCGGACGTACTCCGGCCCCCCTCGGAGCCG‑CCCTCGACTATGTGGGTTCTAAAAATTCTCGCTTCAACTTCGAAAAAATTGcccgggc                                              >  2:237651/1‑135 (MQ=255)                                                                                          tGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGAc                                       <  1:613947/140‑1 (MQ=255)                                                                                          tGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGAc                                       <  1:237651/140‑1 (MQ=255)                                                                                          tGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGAc                                       <  1:1018470/140‑1 (MQ=255)                                                                                                  ggTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTTGCCGGTGCTAACGACCGTCTggc                               >  1:869145/1‑138 (MQ=255)                                                                                                          ccTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGa                       >  2:661560/1‑140 (MQ=255)                                                                                                          ccTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGa                       >  1:1097643/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg                     >  2:804855/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg                     >  1:7982/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg                     >  1:973794/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg                     >  1:29638/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg                     >  1:710982/1‑140 (MQ=255)                                                                                                             cgacgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACt                           <  1:1078606/133‑1 (MQ=255)                                                                                                             cgacgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACt                           >  2:698933/1‑133 (MQ=255)                                                                                                             cgacgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACt                           >  2:1078606/1‑133 (MQ=255)                                                                                                             cgacgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACt                           <  1:698933/133‑1 (MQ=255)                                                                                                               acgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGaaa                  >  1:577185/1‑140 (MQ=255)                                                                                                               acgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGaaa                  >  2:713621/1‑140 (MQ=255)                                                                                                                   aCTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCgg              <  2:480421/140‑1 (MQ=255)                                                                                                                    cTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGt             <  2:577185/140‑1 (MQ=255)                                                                                                                    cTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGt             <  2:274499/140‑1 (MQ=255)                                                                                                                               cATCGCTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGa  <  2:388618/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTC‑GAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGA  >  NC_000913/31704‑31969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑