Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,057,062	G→A	P325S (CCG→TCG)	glnA ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,057,062	0	G	A	100.0%	195.0 / NA	56	P325S (CCG→TCG)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/31);  total (25/31)

TACGGGTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGGGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTGCGAACAGG  >  NC_000913/4056929‑4057192                                                                                                                                      |                                                                                                                                   tACGGGTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAg                                                                                                                              <  1:674776/140‑1 (MQ=255)   cGGGTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGAc                                                                                                                            <  2:790761/140‑1 (MQ=255)   cGGGTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGAc                                                                                                                            <  2:549180/140‑1 (MQ=255)   cGGGTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGAc                                                                                                                            <  2:489810/140‑1 (MQ=255)    gggTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACg                                                                                                                           <  2:154505/140‑1 (MQ=255)     ggTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAg                                                                                                                              >  2:846085/1‑136 (MQ=255)     ggTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAg                                                                                                                              <  1:846085/136‑1 (MQ=255)          gCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTtata                                                                                                                     >  2:898464/1‑140 (MQ=255)          gCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTtata                                                                                                                     >  1:478060/1‑140 (MQ=255)              ccGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAagag                                                                                                                 >  1:829950/1‑140 (MQ=255)               cGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGt                                                                                                                <  2:478060/140‑1 (MQ=255)                                cTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGcc                                                                                               >  1:143192/1‑140 (MQ=255)                                cTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGcc                                                                                               <  2:1011104/140‑1 (MQ=255)                                cTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGcc                                                                                               <  1:8478/140‑1 (MQ=255)                                 ttCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCa                                                                                              <  2:109818/140‑1 (MQ=255)                                     aTACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGcc                                                                                               >  2:465469/1‑135 (MQ=255)                                     aTACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGcc                                                                                               <  1:465469/135‑1 (MQ=255)                                           cgTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAAt                                                                                    >  2:342768/1‑140 (MQ=255)                                             tGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCg                                                                                  >  1:826038/1‑140 (MQ=255)                                             tGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCg                                                                                  >  1:419870/1‑140 (MQ=255)                                             tGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCg                                                                                  >  1:344077/1‑140 (MQ=255)                                               cTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCATAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCt                                                                                >  1:272578/1‑140 (MQ=255)                                                    ggAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGc                                                                           <  1:481235/140‑1 (MQ=255)                                                      agaagaAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCgt                                                                         >  1:29394/1‑140 (MQ=255)                                                        aagaaACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCgtgt                                                                       >  1:607514/1‑140 (MQ=255)                                                             accaccGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGAt                                                                  <  1:804973/140‑1 (MQ=255)                                                                accGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAc                                                               <  2:829950/140‑1 (MQ=255)                                                                accGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAc                                                               <  2:649961/140‑1 (MQ=255)                                                                  cGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgc                                                             >  2:256459/1‑140 (MQ=255)                                                                  cGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgc                                                             <  2:607514/140‑1 (MQ=255)                                                                  cGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgc                                                             <  1:676054/140‑1 (MQ=255)                                                                  cGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgc                                                             <  1:178142/140‑1 (MQ=255)                                                                     aaTACGGATAGACGCAGAACGGTTACGCGCATAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgccgc                                                          <  2:272578/140‑1 (MQ=255)                                                                     aaTACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgccgc                                                          <  2:665947/140‑1 (MQ=255)                                                                     aaTACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgccgc                                                          <  1:597482/140‑1 (MQ=255)                                                                     aaTACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTAcgccgc                                                          <  2:1114462/140‑1 (MQ=255)                                                                       tACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCa                                                        >  1:87062/1‑140 (MQ=255)                                                                          ggATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATg                                                     <  2:143192/140‑1 (MQ=255)                                                                           gATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATgt                                                    <  2:87062/140‑1 (MQ=255)                                                                              aGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATgtagt                                                 >  1:831833/1‑140 (MQ=255)                                                                                     gAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAgcgc                                          <  2:344077/140‑1 (MQ=255)                                                                                      aaCGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCTAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGcc                                         >  1:760395/1‑140 (MQ=255)                                                                                           ttACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTc                                    >  2:315655/1‑140 (MQ=255)                                                                                              cgcgCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTcaga                                 <  2:419870/140‑1 (MQ=255)                                                                                               gcgcAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTcagac                                <  1:247082/140‑1 (MQ=255)                                                                                                cgcAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTcagaca                               <  1:898464/140‑1 (MQ=255)                                                                                                    gagTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGAcc                           <  1:514684/140‑1 (MQ=255)                                                                                                     agTAAGCCAGCATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCt                          >  1:335253/1‑140 (MQ=255)                                                                                                               cATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGt                >  1:755303/1‑140 (MQ=255)                                                                                                               cATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGt                <  2:831833/140‑1 (MQ=255)                                                                                                               cATTACCGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGt                >  2:986146/1‑140 (MQ=255)                                                                                                                    ccGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTTGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCt           >  1:276741/1‑140 (MQ=255)                                                                                                                    ccGGTGCTTCATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCt           >  1:1055709/1‑140 (MQ=255)                                                                                                                         gCTTCATAGCCCTAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTGCGaa      <  2:760395/140‑1 (MQ=255)                                                                                                                         gCTTCATAGCCCGAGACCAGACGCTTATAAGGGGTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGGTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTTCGaa      >  2:108820/1‑140 (MQ=255)                                                                                                                          gttCAAAGCCCGGGACCAGACGCTTAAAAGGGTTGGTGGTTGGGTTTTCCAGGGGGTTAATCCCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCCCAAACAGACCTGCGTATTTGGCCCCTGCGAAc     <  2:276741/139‑1 (MQ=255)                                                                                                                             cATAGCCCGAGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTGCGAACAgg  >  2:468908/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   TACGGGTTAGCTGCCGGATCCGGGAAACGTACTTCGATACGACGTGCTTTCGGAGAAGAAACCACCGGAATACGGATAGACGCAGAACGGTTACGCGCAGAGTAAGCCAGCATTACCGGTGCTTCATAGCCCGGGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTGCGAACAGG  >  NC_000913/4056929‑4057192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑