Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,182	T→C	Y60C (TAC→TGC)	psd ←	phosphatidylserine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,182	0	T	C	100.0%	280.1 / NA	74	Y60C (TAC→TGC)	psd	phosphatidylserine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/46);  total (28/46)

GCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCAT  >  NC_000913/4390045‑4390313                                                                                                                                          |                                                                                                                                    gCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAg                                                                                                                                   >  1:892111/1‑140 (MQ=255)    ggATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTg                                                                                                                                <  1:81437/140‑1 (MQ=255)       ttttATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCg                                                                                                                             <  1:404346/140‑1 (MQ=255)                 ttcGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTTCGGCAGCTGGCGGTGTCCGGCt                                                                                                                   >  1:125950/1‑140 (MQ=255)                   cGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCttt                                                                                                                 <  2:475529/140‑1 (MQ=255)                     aTTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTg                                                                                                               <  2:77963/140‑1 (MQ=255)                        ttACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTgcgc                                                                                                            <  2:987323/140‑1 (MQ=255)                         tACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGcc                                                                                                           >  1:1022924/1‑140 (MQ=255)                          aCCCAACTGGCTGATAACGCCATCGGCAGGCATTACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCGGCTGGCGGTGCCCGGCTTTTGAGCct                                                                                                          >  2:195586/1‑140 (MQ=255)                            ccAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCctct                                                                                                        <  1:1016123/140‑1 (MQ=255)                            ccAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCctct                                                                                                        <  2:423981/140‑1 (MQ=255)                                   gcagATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt                                                                                                 <  2:584032/137‑1 (MQ=255)                                   gCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt                                                                                                 <  2:461705/140‑1 (MQ=255)                                   gCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt                                                                                                 >  2:1089/1‑140 (MQ=255)                                   gCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt                                                                                                 <  2:348203/140‑1 (MQ=255)                                   gCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt                                                                                                 <  2:892111/140‑1 (MQ=255)                                   gCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGt                                                                                                 <  2:174876/140‑1 (MQ=255)                                      gATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGa                                                                                              <  2:731631/140‑1 (MQ=255)                                       aTAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCATGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAc                                                                                             <  2:201045/140‑1 (MQ=255)                                        tAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAcc                                                                                            <  1:303674/140‑1 (MQ=255)                                        tAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAcc                                                                                            <  1:798036/140‑1 (MQ=255)                                        tAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTAAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAcc                                                                                            <  2:948489/140‑1 (MQ=255)                                        tAACGACATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGAcc                                                                                            >  2:798036/1‑140 (MQ=255)                                            gCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgt                                                                                        <  1:762959/140‑1 (MQ=255)                                             ccATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgta                                                                                       <  1:1198067/140‑1 (MQ=255)                                             ccATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgta                                                                                       >  2:59874/1‑140 (MQ=255)                                               aTCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTTCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgtagt                                                                                     <  2:125950/140‑1 (MQ=255)                                                    caggcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTa                                                                                <  1:907310/140‑1 (MQ=255)                                                    caggcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAACTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTa                                                                                <  1:758199/140‑1 (MQ=255)                                                         attaCCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGaa                                                                           <  2:408295/137‑1 (MQ=255)                                                          tGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgtagt                                                                                     >  1:116678/1‑129 (MQ=255)                                                          tGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTgtagt                                                                                     <  2:116678/129‑1 (MQ=255)                                                           gACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACa                                                                         <  1:659462/140‑1 (MQ=255)                                                           gACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACa                                                                         <  2:792082/140‑1 (MQ=255)                                                            aCCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAg                                                                        >  1:323238/1‑140 (MQ=255)                                                             ccAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGa                                                                       >  1:211213/1‑140 (MQ=255)                                                              cAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGAt                                                                      <  1:572622/140‑1 (MQ=255)                                                              cAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGAt                                                                      <  1:195586/140‑1 (MQ=255)                                                                 tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACa                                                                         <  1:68999/134‑1 (MQ=255)                                                                 tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACa                                                                         >  2:68999/1‑134 (MQ=255)                                                                 tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGa                                                                   >  2:111287/1‑140 (MQ=255)                                                                 tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGa                                                                   >  1:771127/1‑140 (MQ=255)                                                                 tACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGa                                                                   >  1:54222/1‑140 (MQ=255)                                                                    aTTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATaa                                                                <  1:556074/140‑1 (MQ=255)                                                                    aTTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATaa                                                                <  1:59874/140‑1 (MQ=255)                                                                      tCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGaa                                                                           <  1:496562/127‑1 (MQ=255)                                                                      tCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGaa                                                                           >  2:496562/1‑127 (MQ=255)                                                                       cGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCa                                                             <  1:1089/140‑1 (MQ=255)                                                                          aTCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGtt                                                          >  2:926287/1‑140 (MQ=255)                                                                           tCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGttt                                                         >  1:235199/1‑140 (MQ=255)                                                                            cGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGtttt                                                        <  2:771127/140‑1 (MQ=255)                                                                            cGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGtttt                                                        <  2:323238/140‑1 (MQ=255)                                                                            cGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGtttt                                                        <  2:54222/140‑1 (MQ=255)                                                                            cGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGtttt                                                        <  2:1022924/140‑1 (MQ=255)                                                                              gTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGt                                                      >  2:540467/1‑140 (MQ=255)                                                                              gTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGt                                                      <  1:933197/140‑1 (MQ=255)                                                                                 tCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAg                                                   <  2:1148272/140‑1 (MQ=255)                                                                                 tCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAg                                                   <  2:235199/140‑1 (MQ=255)                                                                                   gATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGcc                                                 <  1:540467/140‑1 (MQ=255)                                                                                      tGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATc                                              >  1:82356/1‑140 (MQ=255)                                                                                        ggCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCTATCCt                                            >  1:435500/1‑140 (MQ=255)                                                                                        ggCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCa                                                <  1:224523/136‑1 (MQ=255)                                                                                        ggCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCa                                                >  2:224523/1‑136 (MQ=255)                                                                                               ttCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCgct                                     >  1:48955/1‑140 (MQ=255)                                                                                                  gTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCgcttgc                                  >  1:546778/1‑140 (MQ=255)                                                                                                         aGCGGACGGACAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGcc                           >  2:201468/1‑140 (MQ=255)                                                                                                           cggacggaCAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGcccc                         >  2:655357/1‑140 (MQ=255)                                                                                                           cggacggaCAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGcccc                         >  2:694225/1‑140 (MQ=255)                                                                                                            ggacggacAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCa                        >  1:362068/1‑140 (MQ=255)                                                                                                               cggacAAAGAATTCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAAcc                     <  2:211213/140‑1 (MQ=255)                                                                                                                           tCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGt         <  1:694225/140‑1 (MQ=255)                                                                                                                           tCGTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGAAAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGt         <  1:1182465/140‑1 (MQ=255)                                                                                                                             gTTAAAGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTaa       <  2:1024105/140‑1 (MQ=255)                                                                                                                                  aGGTGCGGCAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCAt  >  1:611746/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    GCAGGATTTTATCTTCTTCGATTTTACCCAACTGGCTGATAACGCCATCGGCAGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGATAACCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCAT  >  NC_000913/4390045‑4390313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑