Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,397,446	A→G	N12S (AAC→AGC)	mutL →	DNA mismatch repair protein MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,397,446	0	A	G	100.0%	215.8 / NA	58	N12S (AAC→AGC)	mutL	DNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/22);  total (36/22)

TCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCAT  >  NC_000913/4397311‑4397578                                                                                                                                        |                                                                                                                                     tCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAg                                                                                                                                  >  2:635058/1‑140 (MQ=255) tCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAg                                                                                                                                  >  2:624902/1‑140 (MQ=255)    ttGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGAtt                                                                                                                               >  2:1002334/1‑140 (MQ=255)      gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGc                                                                                                                             >  2:759536/1‑140 (MQ=255)      gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGc                                                                                                                             >  1:1047888/1‑140 (MQ=255)       cgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGcc                                                                                                                            >  1:1058809/1‑140 (MQ=255)        gctcccGATGAAATCGGCGCCGCGGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCGGCCAAACTAAGGACGATTGATGCCAATTCAGGTTTTACCGCCACAACTGGCGAGCCAGATTGCCg                                                                                                                           <  2:455077/136‑1 (MQ=255)         cacccaaTGCAATCGGCGCCGGGGGGTGAAACGGCAAAAACTGCCAGTACGGTGACGACGCCAGATCGCCCGCTCCCAAACTAAGGACGATTGATGCTAATTCAGGTTTTACCGCCACAACTGGCGAGCCAGATTGCCGc                                                                                                                          <  2:863993/134‑1 (MQ=255)                 gCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCaggtgagg                                                                                                                  >  1:540271/1‑140 (MQ=255)                   aaTCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCaggtgaggtg                                                                                                                <  2:540271/140‑1 (MQ=255)                    aTCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGAAGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAggtgg                                                                                                               >  1:706479/1‑140 (MQ=255)                     tCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAggtggt                                                                                                              >  2:1033148/1‑140 (MQ=255)                        gcgcCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGa                                                                                                           >  1:865403/1‑140 (MQ=255)                         cgccgaAGGGTGAACCGGCACAACCTGCCAGGACGTGGCCGACGCCAGATCGCTCCCTGCCAAATTAAGGACATTTGATGCAAATCCAGGTTTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAg                                                                                                          <  2:1047888/140‑1 (MQ=255)                                   tGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGt                                                                                                >  2:1012407/1‑140 (MQ=255)                                             caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaa                                                                                      >  2:460369/1‑140 (MQ=255)                                             caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaa                                                                                      >  1:574333/1‑140 (MQ=255)                                             caAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCaa                                                                                      >  2:182334/1‑140 (MQ=255)                                                aCTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGa                                                                                   >  2:338752/1‑140 (MQ=255)                                                  tGCCAGTACGGTGACGACGCCAGATCGCCCGCTCCCAAACTAAGGACGATTGATGCCAATTCGGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAAc                                                                                 <  2:141931/140‑1 (MQ=255)                                                  tGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAAc                                                                                 <  1:624902/140‑1 (MQ=255)                                                        tACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTgg                                                                           >  1:469560/1‑140 (MQ=255)                                                              gacgtcGCCAGATCGCACGCTGCAAAACTAAGGACGATTGATCCAAATTCAGGTCTTGCCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACa                                                                     <  1:357562/140‑1 (MQ=255)                                                                  ccgCCAGATCGCACGCTGCAAAACTAAGGACGATTGATGCCAATTCGGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCt                                                                 <  2:982834/139‑1 (MQ=255)                                                                  acgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCt                                                                 >  2:247525/1‑140 (MQ=255)                                                                    gCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCg                                                               >  2:156359/1‑140 (MQ=255)                                                                       aGATCGCACGCTGCCACACTAAGGACGATTGATGCCAATTCAGGTCTTATCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGTTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATg                                                            >  2:598854/1‑140 (MQ=255)                                                                       aGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATg                                                            <  1:156359/140‑1 (MQ=255)                                                                       aGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATg                                                            >  1:957848/1‑140 (MQ=255)                                                                              aCGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGc                                                     >  1:1042579/1‑140 (MQ=255)                                                                                  tGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGa                                                              >  2:671721/1‑127 (MQ=255)                                                                                  tGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGa                                                              <  1:671721/127‑1 (MQ=255)                                                                                  tGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcg                                                 <  2:957848/140‑1 (MQ=255)                                                                                  tGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcg                                                 <  2:1007055/140‑1 (MQ=255)                                                                                  tGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcg                                                 <  1:182334/140‑1 (MQ=255)                                                                                      aaaCTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTa                                             <  1:895803/140‑1 (MQ=255)                                                                                        aCTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATc                                           <  1:1033148/140‑1 (MQ=255)                                                                                            aGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGata                                       <  1:338752/140‑1 (MQ=255)                                                                                            aGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGata                                       >  2:505199/1‑140 (MQ=255)                                                                                              gACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                                      >  1:517707/1‑139 (MQ=255)                                                                                              gACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                                      <  2:517707/139‑1 (MQ=255)                                                                                                  aTTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGata                                 <  1:759536/140‑1 (MQ=255)                                                                                                   ttGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGatat                                >  2:1192351/1‑140 (MQ=255)                                                                                                   ttGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGatat                                >  1:894645/1‑140 (MQ=255)                                                                                                        gCCAATTCAGGTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAAc                           >  2:1003134/1‑140 (MQ=255)                                                                                                                 ggTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCCTAGCGATATTGATATCGAACGCGGTggag                  >  2:301949/1‑138 (MQ=255)                                                                                                                  gTCTTACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGc                 <  2:894645/140‑1 (MQ=255)                                                                                                                      tACCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATTGATATTGATATCGAACGCGGTGGGGCGgaa             >  2:738135/1‑140 (MQ=255)                                                                                                                       aCCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAAc            >  1:697831/1‑140 (MQ=255)                                                                                                                       aCCGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAAc            >  2:1086141/1‑140 (MQ=255)                                                                                                                        ccGCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACt           >  2:795018/1‑140 (MQ=255)                                                                                                                          gCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTa         >  2:665773/1‑140 (MQ=255)                                                                                                                          gCCACAACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTa         >  2:1116933/1‑140 (MQ=255)                                                                                                                           gagacattGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTAt        <  1:1006034/133‑1 (MQ=255)                                                                                                                              caACTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCg     <  1:1192351/140‑1 (MQ=255)                                                                                                                                aCTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCa   >  1:59157/1‑140 (MQ=255)                                                                                                                                 cTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCAt  >  1:821954/1‑140 (MQ=255)                                                                                                                                 cTGGCGAGCCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCAt  <  2:706479/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCAT  >  NC_000913/4397311‑4397578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑