Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,426,393	A→G	T96A (ACT→GCT)	rplI →	50S ribosomal subunit protein L9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,426,393	0	A	G	100.0%	200.8 / NA	54	T96A (ACT→GCT)	rplI	50S ribosomal subunit protein L9
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/30);  total (24/30)

GTCGCGCTGAACTGGAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTACTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCG  >  NC_000913/4426256‑4426527                                                                                                                                          |                                                                                                                                       gtcgCGCTGAACTGGAAGCTAAACTTGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCt                                                                                                                                      >  2:878144/1‑140 (MQ=255)   cgcgCTGAACTGGAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTcg                                                                                                                                    <  1:964518/140‑1 (MQ=255)       cTGAACTGGAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGAc                                                                                                                                <  2:948202/140‑1 (MQ=255)       cTGAACTGGAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGAc                                                                                                                                <  1:604144/140‑1 (MQ=255)             tGGAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCt                                                                                                                          <  2:787523/140‑1 (MQ=255)              ggAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTg                                                                                                                         <  1:1139044/140‑1 (MQ=255)               gAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGa                                                                                                                        <  2:508968/140‑1 (MQ=255)                aaGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGAc                                                                                                                       <  1:802021/140‑1 (MQ=255)                            cTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCa                                                                                                           >  2:88348/1‑140 (MQ=255)                            cTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCa                                                                                                           >  1:99782/1‑140 (MQ=255)                              gAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGc                                                                                                         <  2:397930/140‑1 (MQ=255)                              gAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGc                                                                                                         <  2:391991/140‑1 (MQ=255)                               aaGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTTTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCt                                                                                                        <  2:725959/140‑1 (MQ=255)                                   tCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCg                                                                                                    <  1:699414/140‑1 (MQ=255)                                    cTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGt                                                                                                   <  2:99782/140‑1 (MQ=255)                                     tGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCg                                                                                                    >  2:1036413/1‑138 (MQ=255)                                     tGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCg                                                                                                    <  1:1036413/138‑1 (MQ=255)                                            tgctAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTgg                                                                                           >  1:369905/1‑140 (MQ=255)                                             gctAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGc                                                                                          <  1:427882/140‑1 (MQ=255)                                              ctAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCt                                                                                         <  2:1025113/140‑1 (MQ=255)                                                        cgcTGAGAAAATCAATGCACTGGGAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAg                                                                               >  2:282746/1‑140 (MQ=255)                                                        cgcTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAg                                                                               >  2:603593/1‑140 (MQ=255)                                                                       tGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACg                                                                >  1:593292/1‑140 (MQ=255)                                                                                 aCTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCg                                                      <  1:282746/140‑1 (MQ=255)                                                                                 aCTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCg                                                      >  1:191382/1‑140 (MQ=255)                                                                                   tGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACg                                                                <  1:42285/128‑1 (MQ=255)                                                                                   tGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACg                                                                >  2:42285/1‑128 (MQ=255)                                                                                      tACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGaa                                                                  >  2:150639/1‑123 (MQ=255)                                                                                      tACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGaa                                                                  <  1:150639/123‑1 (MQ=255)                                                                                      tACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCa                                                 >  2:470837/1‑140 (MQ=255)                                                                                        ccATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACt                                               >  1:940481/1‑140 (MQ=255)                                                                                          aTCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTgg                                             <  1:1169566/140‑1 (MQ=255)                                                                                            cgcgTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCg                                           <  2:940481/140‑1 (MQ=255)                                                                                            cgcgTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCg                                           <  2:356019/140‑1 (MQ=255)                                                                                              cgTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGaa                                         >  1:297046/1‑140 (MQ=255)                                                                                                tCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAaca                                       >  1:1853/1‑140 (MQ=255)                                                                                                       cTGGTGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTg                                >  1:297865/1‑140 (MQ=255)                                                                                                        tGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGa                               <  1:667324/140‑1 (MQ=255)                                                                                                         ggCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAg                              <  1:603593/140‑1 (MQ=255)                                                                                                          gCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGa                                    <  1:601362/133‑1 (MQ=255)                                                                                                          gCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGa                                    >  2:601362/1‑133 (MQ=255)                                                                                                          gCGACGAAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGc                             >  1:1170910/1‑140 (MQ=255)                                                                                                             acgaAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTc                          <  2:369905/140‑1 (MQ=255)                                                                                                              cgaAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTcc                         <  1:470837/140‑1 (MQ=255)                                                                                                              cgaAGGTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTcc                         >  2:1030687/1‑140 (MQ=255)                                                                                                                  ggTAAACTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGt                     <  1:1078441/140‑1 (MQ=255)                                                                                                                     aaaCTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTca                  <  2:191382/140‑1 (MQ=255)                                                                                                                     aaaCTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTca                  <  1:88348/140‑1 (MQ=255)                                                                                                                     aaaCTGTTCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTca                  <  1:1030687/140‑1 (MQ=255)                                                                                                                            tCGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGaa           >  2:972236/1‑140 (MQ=255)                                                                                                                             cGGTTCCATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAg          >  1:641654/1‑140 (MQ=255)                                                                                                                                   cATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTcg    >  2:325185/1‑140 (MQ=255)                                                                                                                                   cATCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTcg    >  1:306751/1‑140 (MQ=255)                                                                                                                                     tCGGTGCTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTcgcg  >  2:74329/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GTCGCGCTGAACTGGAAGCTAAACTGGCTGAAGTTCTGGCAGCTGCTAATGCTCGCGCTGAGAAAATCAATGCACTGGAAACTGTTACCATCGCGTCTAAAGCTGGCGACGAAGGTAAACTGTTCGGTTCCATCGGTACTCGCGACATCGCTGACGCTGTAACTGCAGCTGGCGTTGAAGTGGCTAAGAGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCG  >  NC_000913/4426256‑4426527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑