Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	373,839	T→C	S307P (TCA→CCA)	mhpF →	acetaldehyde dehydrogenase (acylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	373,839	0	T	C	100.0%	192.7 / NA	52	S307P (TCA→CCA)	mhpF	acetaldehyde dehydrogenase (acylating)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

GGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCAC  >  NC_000913/373708‑373973                                                                                                                                    |                                                                                                                                       ggCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGgcg                                                                                                                                <  2:743010/140‑1 (MQ=255)   cAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGgcgcg                                                                                                                              <  1:187641/140‑1 (MQ=255)     aTTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCa                                                                                                                            >  1:1085543/1‑140 (MQ=255)        ctcCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAgg                                                                                                                         <  2:663565/140‑1 (MQ=255)        ctcCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAgg                                                                                                                         <  2:342810/140‑1 (MQ=255)           cGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAg                                                                                                                      >  2:705531/1‑140 (MQ=255)            ggACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAgg                                                                                                                     >  1:93716/1‑140 (MQ=255)                ggAAACCGGCGGTCTGGCTGGAAGTGGAAGGCGCAGCCCATTATCTGCCTGCCTATGCGGGCAGCCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGaa                                                                                                                 <  2:272147/139‑1 (MQ=255)                  aaaaCAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGc                                                                                                               >  1:914500/1‑140 (MQ=255)                                         cGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTta                                                                                        <  1:444608/140‑1 (MQ=255)                                               cgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtctc                                                                                  >  2:355603/1‑140 (MQ=255)                                               cgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtctc                                                                                  <  2:175810/140‑1 (MQ=255)                                                  agcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGa                                                                               >  1:413337/1‑140 (MQ=255)                                                         tATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTcaca                                                                        >  1:1200125/1‑140 (MQ=255)                                                         tATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTcaca                                                                        <  1:189946/140‑1 (MQ=255)                                                         tATCTGCCTGCCTATGCGCGCACCCTCCAAATTATGACTTCAAGTGCATGGGCGACAGCGAAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTcaca                                                                        <  2:682351/140‑1 (MQ=255)                                                            ctgcctgcCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGTAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTg                                                                     >  1:529200/1‑140 (MQ=255)                                                              gcctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                                                   >  2:5955/1‑140 (MQ=255)                                                              gcctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                                                   <  1:796289/140‑1 (MQ=255)                                                               cctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                                                   <  2:16123/139‑1 (MQ=255)                                                               cctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                                                   >  1:16123/1‑139 (MQ=255)                                                                ctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                                                   >  2:483180/1‑138 (MQ=255)                                                                ctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                                                   <  1:483180/138‑1 (MQ=255)                                                                ctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTg                                                                 >  1:878847/1‑140 (MQ=255)                                                                ctgcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTg                                                                 >  1:1163009/1‑140 (MQ=255)                                                                  gcctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGAc                                                               <  1:355603/140‑1 (MQ=255)                                                                   cctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACg                                                              >  1:427188/1‑140 (MQ=255)                                                                   cctATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCCGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACg                                                              >  1:1017151/1‑140 (MQ=255)                                                                         cGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGc                                                        >  2:989747/1‑140 (MQ=255)                                                                          gggCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCa                                                       <  2:1004005/140‑1 (MQ=255)                                                                          gggCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCa                                                       <  2:1200125/140‑1 (MQ=255)                                                                            gCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACg                                                     >  1:848901/1‑140 (MQ=255)                                                                             cAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAAGGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGc                                                    >  2:204397/1‑140 (MQ=255)                                                                             cAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGc                                                    <  1:517560/140‑1 (MQ=255)                                                                                 cTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCAtt                                                >  1:784445/1‑140 (MQ=255)                                                                                  tCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTc                                               <  1:262536/140‑1 (MQ=255)                                                                                    gACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGCATGCACGCCATTCGt                                             >  1:696360/1‑140 (MQ=255)                                                                                          aTGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAg                                       >  1:748744/1‑140 (MQ=255)                                                                                              cTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTAtt                                   >  2:15623/1‑140 (MQ=255)                                                                                              cTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTAtt                                   <  2:237901/140‑1 (MQ=255)                                                                                              cTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTAtt                                   >  1:237901/1‑140 (MQ=255)                                                                                               ttCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTc                                  <  1:452547/140‑1 (MQ=255)                                                                                                tCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCg                                 >  1:242146/1‑140 (MQ=255)                                                                                                       cgcTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGaaa                          >  1:917236/1‑140 (MQ=255)                                                                                                         cTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAAc                        >  2:515537/1‑140 (MQ=255)                                                                                                         cTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAAc                        >  1:228923/1‑140 (MQ=255)                                                                                                         cTGGCGACAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAAc                        >  1:1110799/1‑140 (MQ=255)                                                                                                               aCAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGc                  <  2:914500/140‑1 (MQ=255)                                                                                                                cAGCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGcc                 <  2:1110799/140‑1 (MQ=255)                                                                                                                  gCGGAAAAAATGGCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAg               <  2:93716/140‑1 (MQ=255)                                                                                                                            gggCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAg     <  2:1085543/139‑1 (MQ=255)                                                                                                                             ggCCCAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGc    >  2:453982/1‑140 (MQ=255)                                                                                                                               cccAGCCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCAc  <  2:917236/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCAC  >  NC_000913/373708‑373973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑