Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	732,070	C→T	R830C (CGT→TGT)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	732,070	0	C	T	100.0%	70.6 / NA	22	R830C (CGT→TGT)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

GACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACC  >  NC_000913/731941‑732124                                                                                                                                  |                                                       gACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACt                                              >  2:452650/1‑140 (MQ=255)     gCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcacc                                          <  1:429035/140‑1 (MQ=255)     gCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcacc                                          <  1:710272/140‑1 (MQ=255)      ccTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcacca                                         <  2:264086/140‑1 (MQ=255)      ccTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcacca                                         >  2:1152126/1‑140 (MQ=255)      ccTGCCCGCCGTGGAATGGCTAACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcacca                                         <  1:489855/140‑1 (MQ=255)       cgccccGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcaccac                                        <  2:177814/137‑1 (MQ=255)       cTGCCCGCCGTGGAATGGCTGCCCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACCCCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcaccac                                        <  2:63671/140‑1 (MQ=255)       cTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTcaccac                                        <  1:375705/140‑1 (MQ=255)           ccgccgTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCt                                    >  1:566174/1‑140 (MQ=255)             gccgTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTta                                  <  2:911334/140‑1 (MQ=255)                    aaTGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCt                           >  1:647243/1‑140 (MQ=255)                        gCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCg                       <  1:1152126/140‑1 (MQ=255)                          tGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCggg                     <  2:647243/140‑1 (MQ=255)                           gcccTACGGCGGGGGCTGGCTGTCAGGAAGGAAACTCGGCGACACCCCGCTGGTGGAGTACCCCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGc                    <  2:283070/138‑1 (MQ=14)                           gACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGc                    >  1:717655/1‑140 (MQ=255)                                       cGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGcc        >  2:1110315/1‑140 (MQ=255)                                       cGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTAAACCCCTGCCGGGCAGTTACAGAGcc        >  1:294619/1‑140 (MQ=255)                                        ggctggctGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGcc        <  2:1104954/139‑1 (MQ=37)                                        ggctggctGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGcc        >  1:1104954/1‑139 (MQ=38)                                        ggctggctGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGAACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCa       >  2:245562/1‑140 (MQ=255)                                             gctgTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCTGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCAcc  >  1:271015/1‑140 (MQ=18)                                                                                                                                  |                                                       GACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACC  >  NC_000913/731941‑732124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑