Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,317	A→G	V55A (GTG→GCG)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,317	0	A	G	100.0%	210.7 / NA	57	V55A (GTG→GCG)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/25);  total (32/25)

CGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCA  >  NC_000913/845180‑845453                                                                                                                                          |                                                                                                                                         cGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGcgca                                                                                                                                        <  2:946822/140‑1 (MQ=255)      aCCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGc                                                                                                                                   <  2:220127/140‑1 (MQ=255)      aCCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGc                                                                                                                                   <  2:458204/140‑1 (MQ=255)           ttGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCa                                                                                                                                 >  2:1134194/1‑137 (MQ=255)           ttGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCa                                                                                                                                 <  1:1134194/137‑1 (MQ=255)                   cGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAg                                                                                                                      >  2:699444/1‑140 (MQ=255)                      tttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGTAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGc                                                                                                                                   <  2:816511/124‑1 (MQ=255)                      tttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGTAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGc                                                                                                                                   >  1:816511/1‑124 (MQ=255)                      tttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGcc                                                                                                                   >  1:1008065/1‑140 (MQ=255)                      tttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGcc                                                                                                                   >  2:810820/1‑140 (MQ=255)                      tttGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGcc                                                                                                                   >  1:675456/1‑140 (MQ=255)                        tGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGc                                                                                                                 >  1:326940/1‑140 (MQ=255)                         gCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCt                                                                                                                <  1:810820/140‑1 (MQ=255)                              tCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCtttttt                                                                                                           >  2:585407/1‑140 (MQ=255)                              tCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCtttttt                                                                                                           >  2:922745/1‑140 (MQ=255)                                gACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGCtttttttg                                                                                                         <  1:575067/140‑2 (MQ=255)                                      cGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGat                                                                                                  >  1:83366/1‑140 (MQ=255)                                           gcggcACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCg                                                                                             <  1:699444/140‑1 (MQ=255)                                                aCTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTcc                                                                                        >  1:1200078/1‑140 (MQ=255)                                                aCTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTcc                                                                                        >  1:751781/1‑140 (MQ=255)                                                   aTTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCggg                                                                                     <  2:528006/140‑1 (MQ=255)                                                          gTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGtt                                                                              <  2:751781/140‑1 (MQ=255)                                                             ccGGTTCTGCAGGGGGCGTGGCGGCGGCGGTGCCCAGCTGTTCGATAAACTCTTTACGCGAGGTGTCATTATCCAGCGCAGCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAg                                                                           <  2:1200078/140‑1 (MQ=255)                                                              cGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGt                                                                          >  2:1138285/1‑140 (MQ=255)                                                              cGGTGCAGCAGGGGGCGTAGCGGCAATGGTGCGAAGCTGGTCGTTCAAGCCTTTACGCGGGGTGTCTTTATCCAGGGCACCCGCCAATGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCTGGTTCCGCGACCGGGTCAga                                                                          >  2:247233/1‑139 (MQ=255)                                                               ggTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGtt                                                                              <  2:606263/135‑1 (MQ=255)                                                               ggTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGtt                                                                              >  1:606263/1‑135 (MQ=255)                                                                gTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGc                                                                                   >  1:322214/1‑129 (MQ=255)                                                                gTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGc                                                                                   <  2:322214/129‑1 (MQ=255)                                                                gTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCg                                                                        >  1:999420/1‑140 (MQ=255)                                                                  tCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGtt                                                                      <  2:782027/140‑1 (MQ=255)                                                                   cagcagGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTg                                                                     <  2:39040/140‑1 (MQ=255)                                                                      cagGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGt                                                                  >  2:860039/1‑140 (MQ=255)                                                                       agGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTc                                                                 <  2:1008065/140‑1 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             <  1:585407/140‑1 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             >  1:452318/1‑140 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             >  2:720132/1‑140 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             >  2:680557/1‑140 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             >  2:543162/1‑140 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             <  1:922745/140‑1 (MQ=255)                                                                           ggCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgtt                                                             >  1:91929/1‑140 (MQ=255)                                                                              gTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtc                                                          >  2:836055/1‑140 (MQ=255)                                                                              gTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtc                                                          >  2:499138/1‑140 (MQ=255)                                                                              gTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtc                                                          >  1:488447/1‑140 (MQ=255)                                                                                aGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtcgt                                                        >  2:323773/1‑140 (MQ=255)                                                                                 gCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtcgtt                                                       <  1:1018904/140‑1 (MQ=255)                                                                                 gCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCcgttgtcgtt                                                       <  1:860039/140‑1 (MQ=255)                                                                                              gcAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCTGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGc                                          <  1:74490/140‑1 (MQ=255)                                                                                              gcAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGc                                          <  2:1027204/140‑1 (MQ=255)                                                                                                aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCg                                        <  2:83366/140‑1 (MQ=255)                                                                                                aaCTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCg                                        <  1:1015569/140‑1 (MQ=255)                                                                                                     gTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTAt                                   >  1:1101653/1‑140 (MQ=255)                                                                                                          tCAACTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATa                              <  2:1101653/140‑1 (MQ=255)                                                                                                             aCTCTTTACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACcg                           >  1:740136/1‑140 (MQ=255)                                                                                                                 tttACGCGAGGTGTCATTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTg                       <  1:1138285/140‑1 (MQ=255)                                                                                                                                aTTATCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCcag        <  2:999420/140‑1 (MQ=255)                                                                                                                                    tCCAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcagg    >  1:629830/1‑140 (MQ=255)                                                                                                                                      cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca  >  1:533193/1‑140 (MQ=255)                                                                                                                                      cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca  <  1:139219/140‑1 (MQ=255)                                                                                                                                      cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca  >  1:1082624/1‑140 (MQ=255)                                                                                                                                      cAGCGCATCCGCCAGTGCGCCATAGGCCGC‑TTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAgcaggca  >  2:980146/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGCAACTGGTCGATCAACTCTTTACGCGAGGTGTCATTATCCAGCACATCCGCCAGTGCGCCATAGGCCGCTTTTTTTTGTTCGATATCCGGTTCCGGGGCCGGTTCAGTCGTTGAGTCCGTTGTCGTTGTGGTTGTAACGCCGGGTATGGATACCGCGTGGGCAGGTGCGCCCAGCAGGCA  >  NC_000913/845180‑845453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑