Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	852,372	A→G	F76L (TTC→CTC)	opgE ←	phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	852,372	0	A	G	100.0%	290.7 / NA	79	F76L (TTC→CTC)	opgE	phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/34);  total (45/34)

CGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTT  >  NC_000913/852235‑852507                                                                                                                                          |                                                                                                                                        cgcAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGAt                                                                                                                                       >  2:537311/1‑140 (MQ=255) cgcAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGAt                                                                                                                                       >  2:38292/1‑140 (MQ=255) cgcAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGAt                                                                                                                                       >  1:520391/1‑140 (MQ=255)                gCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGccacca                                                                                                                        >  2:875229/1‑140 (MQ=255)                 ccGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAg                                                                                                                       <  1:1122210/140‑1 (MQ=255)                 ccGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAg                                                                                                                       <  2:520391/140‑1 (MQ=255)                 ccGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAg                                                                                                                       <  1:138873/140‑1 (MQ=255)                 ccGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAg                                                                                                                       <  1:523805/140‑1 (MQ=255)                    aCCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGa                                                                                                                    >  1:1044294/1‑140 (MQ=255)                    aCCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGa                                                                                                                    >  2:254984/1‑140 (MQ=255)                    aCCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGa                                                                                                                    >  1:665580/1‑140 (MQ=255)                           aGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGaca                                                                                                             <  1:662520/140‑1 (MQ=255)                           aGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGaca                                                                                                             <  2:1044294/140‑1 (MQ=255)                              tGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCg                                                                                                          >  1:590226/1‑140 (MQ=255)                                tACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGAc                                                                                                        >  2:554311/1‑140 (MQ=255)                                 aCCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGAcc                                                                                                       <  2:87506/140‑1 (MQ=255)                                  ccACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTCCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCa                                                                                                      >  1:666540/1‑140 (MQ=255)                                    aCGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGa                                                                                                    >  2:293533/1‑140 (MQ=255)                                     cGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGaa                                                                                                   >  2:353693/1‑140 (MQ=255)                                     cGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGaa                                                                                                   >  2:69836/1‑140 (MQ=255)                                       gAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGaa                                                                                                   <  1:844864/138‑1 (MQ=255)                                       gAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGaa                                                                                                   >  2:844864/1‑138 (MQ=255)                                       gAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACt                                                                                                 >  1:861408/1‑140 (MQ=255)                                       gAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACt                                                                                                 >  2:959444/1‑140 (MQ=255)                                        aaaaaTCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACtt                                                                                                >  2:200099/1‑140 (MQ=255)                                          aaaTCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACtttt                                                                                              >  2:263779/1‑140 (MQ=255)                                                     aTTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAgg                                                                                   <  2:861408/140‑1 (MQ=255)                                                            tCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGtt                                                                            <  1:263779/140‑1 (MQ=255)                                                              gACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTAcgcg                                                                                      <  2:91487/128‑1 (MQ=255)                                                              gACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTAcgcg                                                                                      >  1:91487/1‑128 (MQ=255)                                                                     ccATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATagc                                                                   <  1:501530/140‑1 (MQ=255)                                                                     ccATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATagc                                                                   >  2:501530/1‑140 (MQ=255)                                                                     ccATATTGGTGGAGTCCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATagc                                                                   <  2:666540/140‑1 (MQ=255)                                                                               ggAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAgcaaaagcaaaa                                                         >  1:414042/1‑140 (MQ=255)                                                                                  gTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATagca                                                                  >  2:657605/1‑128 (MQ=255)                                                                                  gTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATagca                                                                  <  1:657605/128‑1 (MQ=255)                                                                                  gTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATac                                                      >  1:650084/1‑139 (MQ=255)                                                                                  gTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAg                                                      >  1:610375/1‑140 (MQ=255)                                                                                   tGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGc                                                     >  2:807654/1‑140 (MQ=255)                                                                                   tGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGc                                                     >  2:864526/1‑140 (MQ=255)                                                                                             gCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCa                                           <  1:353693/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCTGGCTGTAGGCTTGAGCAAAAGGTAGATAACAAGCCGCCGCCAGCGAACTGACCCCGACCAGACCTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  2:590226/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  2:768550/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  1:38292/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  1:293533/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  1:69836/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  2:414042/140‑1 (MQ=255)                                                                                              cAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  1:623766/140‑1 (MQ=255)                                                                                               aaTGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGc                                         >  2:695464/1‑140 (MQ=255)                                                                                                aTGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCg                                        >  1:468022/1‑140 (MQ=255)                                                                                                  gTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGgt                                      <  1:537311/140‑1 (MQ=255)                                                                                                        aaaTTAGGCGCGCCGTAGGCCTGAGCAAAGGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGATGTagag                                >  1:42193/1‑140 (MQ=255)                                                                                                        aaaTTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTagag                                <  2:854084/140‑1 (MQ=255)                                                                                                        aaaTTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTagag                                >  1:522189/1‑140 (MQ=255)                                                                                                          aTTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCa                              <  2:522189/140‑1 (MQ=255)                                                                                                             aGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAAc                           >  2:55439/1‑140 (MQ=255)                                                                                                             aGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAAc                           >  2:685174/1‑140 (MQ=255)                                                                                                             aGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAAc                           >  2:98658/1‑140 (MQ=255)                                                                                                             aGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAAc                           >  2:517287/1‑140 (MQ=255)                                                                                                             aGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAAc                           >  2:415117/1‑140 (MQ=255)                                                                                                             aGGCGCGCCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAAc                           >  2:1199397/1‑140 (MQ=255)                                                                                                               gcgcgcCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          <  2:1197813/123‑1 (MQ=255)                                                                                                               gcgcgcCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAg                                          >  1:1197813/1‑123 (MQ=255)                                                                                                               gcgcgcCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTg                         <  1:200099/140‑1 (MQ=255)                                                                                                               gcgcgcCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTg                         <  1:251348/140‑1 (MQ=255)                                                                                                                  cgcCGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAg                      >  2:612716/1‑140 (MQ=255)                                                                                                                    ccGTAGGCCTGAGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAggg                    <  1:554311/140‑1 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTg                         >  2:1197701/1‑124 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTg                         <  1:1197701/124‑1 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc         >  2:514534/1‑140 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc         >  1:1126019/1‑140 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc         >  1:188884/1‑140 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc         >  2:834305/1‑140 (MQ=255)                                                                                                                               aGCAAAAGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc         >  2:1144434/1‑140 (MQ=255)                                                                                                                                  aaaaGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGa      <  2:1126019/140‑1 (MQ=255)                                                                                                                                  aaaaGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGa      <  2:96496/140‑1 (MQ=255)                                                                                                                                    aaGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAgg    <  1:612716/140‑1 (MQ=255)                                                                                                                                    aaGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAgg    <  1:685174/140‑1 (MQ=255)                                                                                                                                    aaGGGAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAgg    <  2:665580/140‑1 (MQ=255)                                                                                                                                      gggAGATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGtt  >  1:1040424/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCCATAGCAAAAGCAAAATAGCCGTAAACGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTT  >  NC_000913/852235‑852507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑