Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,637	A→G	E83E (GAA→GAG)	ycaQ →	winged helix DNA‑binding domain‑containing protein YcaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,637	0	A	G	100.0%	248.8 / NA	66	E83E (GAA→GAG)	ycaQ	winged helix DNA‑binding domain‑containing protein YcaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/32);  total (34/32)

CTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACA  >  NC_000913/969502‑969771                                                                                                                                        |                                                                                                                                       ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATC‑GGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTg                                                                                                                                   <  1:171472/140‑1 (MQ=255)   cccGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTgg                                                                                                                                  >  2:929980/1‑140 (MQ=255)    ccGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTggg                                                                                                                                 >  1:653320/1‑140 (MQ=255)       cATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGgcgc                                                                                                                              <  2:290092/140‑1 (MQ=255)        aTGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCa                                                                                                                             <  2:293909/140‑1 (MQ=255)          gTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATg                                                                                                                           >  1:657061/1‑140 (MQ=255)              ttGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGc                                                                                                                       >  2:442228/1‑140 (MQ=255)              ttGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGc                                                                                                                       <  1:552195/140‑1 (MQ=255)              ttGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGc                                                                                                                       <  1:574341/140‑1 (MQ=255)                gctgcAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCt                                                                                                                     >  1:60705/1‑140 (MQ=255)                 ctgcAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTg                                                                                                                    >  1:697997/1‑140 (MQ=255)                     aaaTCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTggg                                                                                                                                 <  2:67887/123‑1 (MQ=255)                     aaaTCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTggg                                                                                                                                 >  1:67887/1‑123 (MQ=255)                                 tCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGc                                                                                                    >  1:305202/1‑140 (MQ=255)                                      atTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACtt                                                                                               <  2:697997/140‑1 (MQ=255)                                             cccGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGTCGCGTAGCGACTTTCGTCtt                                                                                        >  2:616807/1‑140 (MQ=255)                                             cccGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCtt                                                                                        >  1:706294/1‑140 (MQ=255)                                               cGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTAt                                                                                      >  1:97713/1‑140 (MQ=255)                                               cGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTAt                                                                                      >  2:1045220/1‑140 (MQ=255)                                                 tAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTc                                                                                    >  1:100752/1‑140 (MQ=255)                                                    tCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGcc                                                                                 >  1:79697/1‑140 (MQ=255)                                                         atCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGc                                                                            >  1:690171/1‑140 (MQ=255)                                                            tGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATg                                                                         >  1:872559/1‑140 (MQ=255)                                                              gTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCt                                                                       >  2:538653/1‑140 (MQ=255)                                                                           gtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGaaaaa                                                          >  1:686982/1‑140 (MQ=255)                                                                              tGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATg                                                       >  1:1002725/1‑140 (MQ=255)                                                                               gggAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATgg                                                      <  1:1045220/140‑1 (MQ=255)                                                                                ggAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATggg                                                     >  2:488839/1‑140 (MQ=255)                                                                                ggAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATggg                                                     >  1:954156/1‑140 (MQ=255)                                                                                 gAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGc                                                    <  1:345194/140‑1 (MQ=255)                                                                                 gAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGc                                                    <  2:706294/140‑1 (MQ=255)                                                                                    aTTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTgg                                                 <  2:657061/140‑1 (MQ=255)                                                                                    aTTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTgg                                                 <  2:305202/140‑1 (MQ=255)                                                                                    aTTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTgg                                                 <  1:269115/140‑1 (MQ=255)                                                                                    aTTATCCCGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTgg                                                 <  2:457036/140‑1 (MQ=255)                                                                                     ttATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGa                                                <  2:690171/140‑1 (MQ=255)                                                                                     ttATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGa                                                <  1:999317/140‑1 (MQ=255)                                                                                          cTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATAc                                           >  2:431794/1‑140 (MQ=255)                                                                                             cccAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACaaa                                        >  2:424788/1‑140 (MQ=255)                                                                                               cAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGa                                      <  2:521647/140‑1 (MQ=255)                                                                                                  tGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGc                                   <  2:1162927/140‑1 (MQ=255)                                                                                                    gCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCt                                 <  1:442228/140‑1 (MQ=255)                                                                                                    gCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCt                                 <  2:60705/140‑1 (MQ=255)                                                                                                            aGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAg                         >  2:615422/1‑140 (MQ=255)                                                                                                            aGTCTCTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAg                         >  2:755922/1‑140 (MQ=255)                                                                                                              tctcTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACaaa                                        >  2:438865/1‑123 (MQ=255)                                                                                                              tctcTGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACaaa                                        <  1:438865/123‑1 (MQ=255)                                                                                                              tctcTGGCGCGTGGCGAATTAATGGAGTACT‑GGCGCATGAAGGCTGCTTTATGCCGCGGAACCGCTTTCGGCCTATTCGCCACCGGATGCTTGCACCCGGAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGaa                      >  2:677582/1‑140 (MQ=255)                                                                                                                tctGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAAc                     >  2:228682/1‑140 (MQ=255)                                                                                                                tctGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAAc                     <  2:686982/140‑1 (MQ=255)                                                                                                                  tGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACAt                   <  2:653320/140‑1 (MQ=255)                                                                                                                  tGGCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACAt                   <  1:929980/140‑1 (MQ=255)                                                                                                                   ggCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCt                                 >  2:771172/1‑125 (MQ=255)                                                                                                                   ggCGCGTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCt                                 <  1:771172/125‑1 (MQ=255)                                                                                                                       cgTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGc              <  2:1002725/140‑1 (MQ=255)                                                                                                                       cgTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGc              <  1:977172/140‑1 (MQ=255)                                                                                                                        gTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGa                              >  2:1167280/1‑123 (MQ=255)                                                                                                                        gTGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGa                              <  1:1167280/123‑1 (MQ=255)                                                                                                                         tGGCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCgg            >  1:206498/1‑140 (MQ=255)                                                                                                                           gCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATg                            >  1:186331/1‑122 (MQ=255)                                                                                                                           gCGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATg                            <  2:186331/122‑1 (MQ=255)                                                                                                                            cGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGaaa         >  1:436252/1‑140 (MQ=255)                                                                                                                            cGAATTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGaaa         <  2:79697/140‑1 (MQ=255)                                                                                                                              aaTTAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAAtt       >  1:622246/1‑140 (MQ=255)                                                                                                                                ttAATGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGc     <  1:431794/140‑1 (MQ=255)                                                                                                                                   aTGGAGTACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGcaca  <  1:424788/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACA  >  NC_000913/969502‑969771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑