Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,387	A→G	T168A (ACC→GCC)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,387	0	A	G	100.0%	210.6 / NA	57	T168A (ACC→GCC)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/34);  total (23/34)

ATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTA  >  NC_000913/1098259‑1098521                                                                                                                                 |                                                                                                                                       aTTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCg                                                                                                                             >  1:399802/1‑140 (MQ=255)        aCCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTg                                                                                                                      >  1:117578/1‑140 (MQ=255)             tGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTg                                                                                                                 <  2:399802/140‑1 (MQ=255)               ccTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCa                                                                                                               >  1:290091/1‑140 (MQ=255)                  gAATATCATCGGGAAGATTTTACCATCGGGATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGGGGCCTGGCGTGCAAAg                                                                                                            <  2:264772/140‑1 (MQ=255)                       tcatcGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTg                                                                                                       >  2:84792/1‑140 (MQ=255)                         atcGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGcc                                                                                                     >  2:670251/1‑140 (MQ=255)                               aaGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACgg                                                                                               >  2:1105821/1‑140 (MQ=255)                                  aTTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGgaa                                                                                            >  2:320682/1‑140 (MQ=255)                                     ttACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGgaagaa                                                                                         <  1:114598/140‑1 (MQ=255)                                      tACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAAc                                                                                        >  1:920015/1‑140 (MQ=255)                                       aCCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACt                                                                                       <  1:1105821/140‑1 (MQ=255)                                       aCCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACt                                                                                       <  2:802873/140‑1 (MQ=255)                                        ccATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTg                                                                                      <  1:84792/140‑1 (MQ=255)                                            cGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACt                                                                                       >  1:296972/1‑135 (MQ=255)                                            cGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACt                                                                                       <  2:296972/135‑1 (MQ=255)                                             ggCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGc                                                                                 <  1:823874/140‑1 (MQ=255)                                             ggCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGc                                                                                 >  2:741914/1‑140 (MQ=255)                                             ggCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGc                                                                                 >  2:925003/1‑140 (MQ=255)                                              gCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCa                                                                                <  2:948099/140‑1 (MQ=255)                                                  tttGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTc                                                                            <  2:654221/140‑1 (MQ=255)                                                  tttGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTc                                                                            <  1:1061812/140‑1 (MQ=255)                                                      ggCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAg                                                                        <  2:706920/140‑1 (MQ=255)                                                      ggCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAg                                                                        <  1:925003/140‑1 (MQ=255)                                                       gcgcAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGc                                                                       <  1:965500/140‑1 (MQ=255)                                                        cgcAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGcc                                                                      <  1:913833/140‑1 (MQ=255)                                                         gcAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCa                                                                     <  1:320682/140‑1 (MQ=255)                                                         gcAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCa                                                                     <  2:752453/140‑1 (MQ=255)                                                         gcAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCa                                                                     <  1:622637/140‑1 (MQ=255)                                                           aGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAAt                                                                   <  2:845536/140‑1 (MQ=255)                                                                    gggCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTAt                                                          <  1:1170907/140‑1 (MQ=255)                                                                       cAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGa                                                       >  2:1086891/1‑140 (MQ=255)                                                                        aGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGAt                                                      <  1:636581/140‑1 (MQ=255)                                                                        aGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGAt                                                      <  1:868112/140‑1 (MQ=255)                                                                          tAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTc                                                                >  1:1077880/1‑128 (MQ=255)                                                                          tAAACTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTc                                                                <  2:1077880/128‑1 (MQ=255)                                                                               ttGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTc                                                                <  2:1053375/123‑1 (MQ=255)                                                                               ttGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTc                                                                >  1:1053375/1‑123 (MQ=255)                                                                               ttGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTg                                               <  2:42615/140‑1 (MQ=255)                                                                               ttGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTg                                               >  1:1074298/1‑140 (MQ=255)                                                                                  cTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTa                                            <  2:131536/140‑1 (MQ=255)                                                                                  cTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTa                                            <  1:1132336/140‑1 (MQ=255)                                                                                  cTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTa                                            <  2:644241/140‑1 (MQ=255)                                                                                       agTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGaa                                       <  1:962549/140‑1 (MQ=255)                                                                                        gTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGTGTATTGATTAATTTGTTACCGAAt                                      <  1:670251/140‑1 (MQ=255)                                                                                        gTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAAt                                      <  1:817210/140‑1 (MQ=255)                                                                                           tGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATAcc                                   >  1:548602/1‑140 (MQ=255)                                                                                            gCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGTACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATAccc                                  >  1:17637/1‑140 (MQ=255)                                                                                                 ccTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGaa                             >  2:69261/1‑140 (MQ=255)                                                                                                 ccTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGaa                             >  1:529482/1‑140 (MQ=255)                                                                                                   tGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGaa                                       >  1:15756/1‑128 (MQ=255)                                                                                                   tGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGaa                                       <  2:15756/128‑1 (MQ=255)                                                                                                   tGGCGCTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAAc                           >  1:840327/1‑140 (MQ=255)                                                                                                      cgcTTTCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGt                        <  1:741914/140‑1 (MQ=255)                                                                                                         tttCCGCTGCGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCAGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCgg                     <  2:920015/140‑1 (MQ=255)                                                                                                                  cGTTGCTGGAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTaa            >  2:14314/1‑140 (MQ=255)                                                                                                                          gAGTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAAt    <  2:290091/140‑1 (MQ=255)                                                                                                                            gTCGAGCCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTa  >  2:561942/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       ATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTA  >  NC_000913/1098259‑1098521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑