Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,140,047	A→C	T5T (ACA→ACC)	flgL →	flagellar hook‑filament junction protein 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,140,047	0	A	C	100.0%	285.7 / NA	75	T5T (ACA→ACC)	flgL	flagellar hook‑filament junction protein 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/38);  total (37/38)

GATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATCACAAGCCGTA  >  NC_000913/1139914‑1140182                                                                                                                                      |                                                                                                                                        gATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAgatg                                                                                                                                   >  1:1133415/1‑140 (MQ=255)      ggagTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTa                                                                                                                              <  2:1133415/140‑1 (MQ=255)      ggagTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTa                                                                                                                              <  1:829076/140‑1 (MQ=255)       gagTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTGCCCAGATGATGTAc                                                                                                                             >  2:456708/1‑140 (MQ=255)         gTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCa                                                                                                                           >  1:1098253/1‑140 (MQ=255)            cGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCa                                                                                                                        >  1:543278/1‑140 (MQ=255)                aaTCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAAc                                                                                                                    <  2:543278/140‑1 (MQ=255)                 aTCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACa                                                                                                                   >  2:377252/1‑140 (MQ=255)                  tCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACAt                                                                                                                  >  1:1168753/1‑140 (MQ=255)                  tCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACAt                                                                                                                  >  2:60211/1‑140 (MQ=255)                   cTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATg                                                                                                                 <  2:1098253/140‑1 (MQ=255)                    tGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGc                                                                                                                >  1:932794/1‑140 (MQ=255)                    tGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGc                                                                                                                <  1:377252/140‑1 (MQ=255)                     gCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCg                                                                                                               <  2:65598/140‑1 (MQ=255)                      cAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCg                                                                                                               <  1:992229/139‑1 (MQ=255)                      cAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCg                                                                                                               >  2:992229/1‑139 (MQ=255)                           ttttCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTGCCCAGATGATGTACCAGCAAAACATGCGTGGTAt                                                                                                         <  1:456708/140‑1 (MQ=255)                             ttCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCa                                                                                                       >  1:1016292/1‑140 (MQ=255)                                agcagTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCa                                                                                                    >  1:987892/1‑140 (MQ=255)                                         aCCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           <  1:471604/140‑1 (MQ=255)                                         aCCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           <  1:60211/140‑1 (MQ=255)                                         aCCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           <  1:823069/140‑1 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGTAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  2:37891/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           >  2:624345/1‑134 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           <  1:624345/134‑1 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           <  2:871838/134‑1 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           <  1:823616/134‑1 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           >  1:871838/1‑134 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  2:420967/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  2:1131881/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  2:88790/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  2:95467/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  1:1055290/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  1:893730/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAAt                                                                                     >  1:125036/1‑140 (MQ=255)                                               cGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGAGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAgg                                                                                           >  2:823616/1‑134 (MQ=255)                                                 aaTGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAg                                                                                        <  2:657000/135‑1 (MQ=255)                                                 aaTGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAg                                                                                        >  1:657000/1‑135 (MQ=255)                                                  aTGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGa                                                                                  <  1:1131881/140‑1 (MQ=255)                                                        aGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGt                                                                            <  2:125036/140‑1 (MQ=255)                                                        aGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGt                                                                            <  2:298592/140‑1 (MQ=255)                                                                     ggCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGaa                                                                    <  1:431542/135‑1 (MQ=255)                                                                     ggCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGaa                                                                    >  2:431542/1‑135 (MQ=255)                                                                          aCGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGa                                                          <  2:407360/140‑1 (MQ=255)                                                                          aCGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGa                                                          >  2:390248/1‑140 (MQ=255)                                                                           cgcgATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGAc                                                         <  2:932794/140‑1 (MQ=255)                                                                              gATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACggg                                                      <  1:420967/140‑1 (MQ=255)                                                                               aTTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGt                                                     >  2:429043/1‑140 (MQ=255)                                                                                     gATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGa                                               <  1:390248/140‑1 (MQ=255)                                                                                        gcgcTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTc                                            <  1:429043/140‑1 (MQ=255)                                                                                           cTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGtt                                         <  1:954768/140‑1 (MQ=255)                                                                                           cTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGtt                                         <  2:1016292/140‑1 (MQ=255)                                                                                             gATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTaa                                       <  2:903752/140‑1 (MQ=255)                                                                                             gATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTaa                                       <  2:120025/140‑1 (MQ=255)                                                                                                   cATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTc                                 >  2:759195/1‑140 (MQ=255)                                                                                                      tCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGa                              >  2:302566/1‑140 (MQ=255)                                                                                                      tCGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGa                              >  2:1049302/1‑140 (MQ=255)                                                                                                       cGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGAc                             <  1:949341/140‑1 (MQ=255)                                                                                                       cGCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGAc                             <  1:37891/140‑1 (MQ=255)                                                                                                        gCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGtt                                         >  1:367931/1‑127 (MQ=255)                                                                                                        gCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGtt                                         <  2:367931/127‑1 (MQ=255)                                                                                                        gCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGAc                             >  1:348795/1‑139 (MQ=255)                                                                                                        gCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGAc                             <  2:348795/139‑1 (MQ=255)                                                                                                        gCTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACg                            >  2:242016/1‑140 (MQ=255)                                                                                                         cTAAGGGGAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGa                           <  1:88790/140‑1 (MQ=255)                                                                                                              gggAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTaa                                       <  1:341372/123‑1 (MQ=255)                                                                                                              gggAGATAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTaa                                       >  2:341372/1‑123 (MQ=255)                                                                                                                 agaTAAGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATtg                   >  2:253853/1‑140 (MQ=255)                                                                                                                     aaGATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATtgctgc               <  1:302566/140‑1 (MQ=255)                                                                                                                         tGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATca           >  2:326762/1‑140 (MQ=255)                                                                                                                         tGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATca           >  2:199194/1‑140 (MQ=255)                                                                                                                             tttCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATCACAAg       >  1:278084/1‑140 (MQ=255)                                                                                                                             tttCAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATCACAAg       <  1:270658/140‑1 (MQ=255)                                                                                                                                cAGTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATCACAAGCCg    <  2:620839/140‑1 (MQ=255)                                                                                                                                  gTACCCAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATCACAAGCCGTa  <  1:66727/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        GATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACCAATTCTCAGGCAGAATGGATGAAGTACGGCGAACAGATGTCGACGGGTAAGCGAGTCGTTAACCCTTCTGACGATCCCATTGCTGCATCACAAGCCGTA  >  NC_000913/1139914‑1140182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑