Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,066	C→T	A149V (GCC→GTC)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,066	0	C	T	100.0%	198.0 / NA	58	A149V (GCC→GTC)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/22);  total (36/22)

GTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGA  >  NC_000913/1147931‑1148201                                                                                                                                        |                                                                                                                                        gTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAAc                                                                                                                                     >  1:149040/1‑140 (MQ=255)  tGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACg                                                                                                                                    <  2:444791/140‑1 (MQ=255)    ccGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTc                                                                                                                                  >  2:155640/1‑140 (MQ=255)          aTACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATtgt                                                                                                                            <  2:1142052/140‑1 (MQ=255)            aCCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGa                                                                                                                          <  1:155640/140‑1 (MQ=255)            aCCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGa                                                                                                                          >  2:8125/1‑140 (MQ=255)                  gcgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATCACCACATCCGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGcac                                                                                                                    >  2:176203/1‑140 (MQ=255)                            ggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTg                                                                                                          >  1:508507/1‑140 (MQ=255)                                ggCAAAATTATTACTCAAGCCCCTGGAGGGGCTTGAGCGTCCGGCGCTGGTGACGGAATTACCACATCAGCAAAAGGGCAAAACGGTGGTTCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAAt                                                                                                      >  2:256613/1‑140 (MQ=255)                                ggCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAAt                                                                                                      >  1:445647/1‑140 (MQ=255)                                  cAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAAttt                                                                                                    >  1:684068/1‑140 (MQ=255)                                       ttattaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCAt                                                                                               >  2:79858/1‑140 (MQ=255)                                           taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATg                                                                                           >  1:1170779/1‑140 (MQ=255)                                                 aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCa                                                                                     >  2:833657/1‑140 (MQ=255)                                                  gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAg                                                                                    >  2:765605/1‑140 (MQ=255)                                                  gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAg                                                                                    >  2:562374/1‑140 (MQ=255)                                                  gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAg                                                                                    >  2:556250/1‑140 (MQ=255)                                                       tGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTg                                                                               >  2:822117/1‑140 (MQ=255)                                                        ggAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTgg                                                                              >  2:271040/1‑140 (MQ=255)                                                            gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGa                                                                          >  2:30959/1‑140 (MQ=255)                                                             ggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGaa                                                                         >  2:243377/1‑140 (MQ=255)                                                              gATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAag                                                                        >  2:119549/1‑140 (MQ=255)                                                                ttGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAagag                                                                      <  1:30959/140‑1 (MQ=255)                                                                   aGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAagag                                                                      >  1:1068482/1‑137 (MQ=255)                                                                   aGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAagag                                                                      <  2:1068482/137‑1 (MQ=255)                                                                   aGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAggtg                                                                   >  1:490309/1‑140 (MQ=255)                                                                        ccGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGa                                                              >  2:898491/1‑140 (MQ=255)                                                                        ccGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGa                                                              >  2:179413/1‑140 (MQ=255)                                                                        ccGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGa                                                              <  1:565951/140‑1 (MQ=255)                                                                                         aTTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTcgcg                                             <  2:859509/140‑1 (MQ=255)                                                                                           tACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTg                                           >  2:439800/1‑140 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGTTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  1:179413/140‑1 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  2:950032/140‑1 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  1:119549/140‑1 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  1:898491/140‑1 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  1:833657/140‑1 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  1:822117/140‑1 (MQ=255)                                                                                              cacaTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                        <  2:508507/140‑1 (MQ=255)                                                                                                caTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGtt                                      >  1:827711/1‑140 (MQ=255)                                                                                                caTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGtt                                      <  2:445647/140‑1 (MQ=255)                                                                                                 aTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTg                                     >  2:797631/1‑140 (MQ=255)                                                                                                  tCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGc                                    >  1:509198/1‑140 (MQ=255)                                                                                                   cAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCt                                   <  1:998775/140‑1 (MQ=255)                                                                                                    aGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTc                                  >  2:815096/1‑140 (MQ=255)                                                                                                     gCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCa                                 >  2:586855/1‑140 (MQ=255)                                                                                                      cAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCaa                                >  2:238410/1‑140 (MQ=255)                                                                                                        aaaGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAata                              <  1:243377/140‑1 (MQ=255)                                                                                                            ggCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTgg                          <  2:656450/140‑1 (MQ=255)                                                                                                            ggCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTgg                          >  1:656450/1‑140 (MQ=255)                                                                                                                      tggtCCTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTgaagaagaa                >  2:934412/1‑140 (MQ=255)                                                                                                                          ccTTGACTTAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTaa            <  2:1170779/140‑1 (MQ=255)                                                                                                                                  tAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGtctc    >  2:225760/1‑140 (MQ=255)                                                                                                                                  tAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGtctc    >  2:207718/1‑140 (MQ=255)                                                                                                                                  tAGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGtctc    >  2:975774/1‑140 (MQ=255)                                                                                                                                   aGGGGTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCg   >  2:889928/1‑140 (MQ=255)                                                                                                                                    ggggTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGa  <  1:271040/140‑1 (MQ=255)                                                                                                                                    ggggTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGa  <  1:256613/140‑1 (MQ=255)                                                                                                                                    ggggTCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGa  <  1:797631/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGA  >  NC_000913/1147931‑1148201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑