Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,174,377	A→G	G263G (GGT→GGC)	ycfT ←	inner membrane protein YcfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,377	0	A	G	100.0%	261.2 / NA	69	G263G (GGT→GGC)	ycfT	inner membrane protein YcfT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/32);  total (37/32)

CTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGC  >  NC_000913/1174244‑1174513                                                                                                                                      |                                                                                                                                         cTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAAt                                                                                                                                    <  2:322300/140‑1 (MQ=255) cTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAAt                                                                                                                                    <  2:492209/140‑1 (MQ=255)        cgACCAGCGTGCTGCGTACATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCt                                                                                                                             <  2:164562/140‑1 (MQ=255)           ccAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGt                                                                                                                          <  2:800369/140‑1 (MQ=255)           ccAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGt                                                                                                                          <  1:960743/140‑1 (MQ=255)           ccAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGt                                                                                                                          <  2:1170666/140‑1 (MQ=255)           ccAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGt                                                                                                                          <  2:250262/140‑1 (MQ=255)           ccAGCGTGCTGCGTTCACTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGt                                                                                                                          <  2:1070624/140‑1 (MQ=255)               cGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTg                                                                                                                      >  1:426349/1‑140 (MQ=255)                      cGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAAc                                                                                                               >  1:351995/1‑140 (MQ=255)                        ttCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAg                                                                                                             >  1:848686/1‑140 (MQ=255)                        ttCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAg                                                                                                             >  1:1201713/1‑140 (MQ=255)                         tCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGc                                                                                                            <  2:354578/140‑1 (MQ=255)                         tCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGc                                                                                                            >  2:1160586/1‑140 (MQ=255)                                      cAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcacca                                                                                               <  1:804830/140‑1 (MQ=255)                                      cAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcacca                                                                                               <  2:1061794/140‑1 (MQ=255)                                      cAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcacca                                                                                               <  1:1141294/140‑1 (MQ=255)                                              aGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGAt                                                                                       >  1:750915/1‑140 (MQ=255)                                                  gAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGGATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCa                                                                                   <  2:602428/140‑1 (MQ=255)                                                   aaTATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcacca                                                                                               >  2:1175792/1‑127 (MQ=255)                                                   aaTATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATcacca                                                                                               <  1:1175792/127‑1 (MQ=255)                                                   aaTATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAg                                                                                  >  1:508285/1‑140 (MQ=255)                                                          cAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGacaac                                                                           <  2:688875/140‑1 (MQ=255)                                                               aGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAAcagcag                                                                      <  2:1095270/140‑1 (MQ=255)                                                               aGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAAcagcag                                                                      <  2:426349/140‑1 (MQ=255)                                                                    gCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCgaga                                                                 <  2:848686/140‑1 (MQ=255)                                                                         gggTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTa                                                            <  1:841661/140‑1 (MQ=255)                                                                           gtggtgTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCgaga                                                                 <  2:238817/133‑1 (MQ=255)                                                                           gtggtgTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCgaga                                                                 >  1:238817/1‑133 (MQ=255)                                                                           gtggtgTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTaaa                                                          >  2:79438/1‑140 (MQ=255)                                                                           gtggtgTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTaaa                                                          <  1:1160586/140‑1 (MQ=255)                                                                                      aGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTaaa                                                          >  2:314928/1‑129 (MQ=255)                                                                                      aGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTaaa                                                          <  1:314928/129‑1 (MQ=255)                                                                                      aGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCac                                               >  1:963999/1‑140 (MQ=255)                                                                                       gCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCacc                                              <  2:508285/140‑1 (MQ=255)                                                                                        cAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAaacaac                                                      >  2:1185869/1‑131 (MQ=255)                                                                                        cAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAaacaac                                                      <  1:1185869/131‑1 (MQ=255)                                                                                        cAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccg                                             >  2:938565/1‑140 (MQ=255)                                                                                        cAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccg                                             >  1:1155535/1‑140 (MQ=255)                                                                                        cAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccg                                             >  1:849847/1‑140 (MQ=255)                                                                                        cAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccg                                             >  1:751941/1‑140 (MQ=255)                                                                                         aaTGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccga                                            >  1:1112831/1‑140 (MQ=255)                                                                                         aaTGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccga                                            >  1:890689/1‑140 (MQ=255)                                                                                         aaTAGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccga                                            >  1:866968/1‑140 (MQ=255)                                                                                          aTGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccgac                                           <  1:196831/140‑1 (MQ=255)                                                                                          aTGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccgac                                           <  1:306355/140‑1 (MQ=255)                                                                                           tGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccgac                                           >  1:321894/1‑139 (MQ=255)                                                                                           tGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccgac                                           <  2:321894/139‑1 (MQ=255)                                                                                             gtgtTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccg                                             >  2:492477/1‑135 (MQ=255)                                                                                             gtgtTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCaccg                                             <  1:492477/135‑1 (MQ=255)                                                                                             gtgtTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGc                                        >  2:1119118/1‑140 (MQ=255)                                                                                              tgtTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGcc                                       >  2:1024999/1‑140 (MQ=255)                                                                                                  ggAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGaa                                   >  1:917216/1‑140 (MQ=255)                                                                                                   gAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAAc                                  <  2:917216/140‑1 (MQ=255)                                                                                                     gCCAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACgg                                >  1:794132/1‑140 (MQ=255)                                                                                                      ccAATCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGt                               <  2:849847/140‑1 (MQ=255)                                                                                                        aaTCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTaa                             >  2:5113/1‑140 (MQ=255)                                                                                                        aaTCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTa                              <  1:1073949/139‑1 (MQ=255)                                                                                                        aaTCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCATCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTa                              >  2:1073949/1‑139 (MQ=255)                                                                                                          tCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAAc                                  >  1:666695/1‑133 (MQ=255)                                                                                                          tCACATTCAGCAGGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAAc                                  <  2:666695/133‑1 (MQ=255)                                                                                                                  agcagGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGc                   >  2:684071/1‑140 (MQ=255)                                                                                                                  agcagGCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGc                   >  2:300461/1‑140 (MQ=255)                                                                                                                     catcTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTGCATGATCCCCACAATCGATACCAGCGACAATAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCag                <  2:739151/137‑2 (MQ=255)                                                                                                                       gCTGGTGGAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATca              >  2:1165523/1‑140 (MQ=255)                                                                                                                            tggAGCGCATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGa         >  1:946707/1‑140 (MQ=255)                                                                                                                                 cgcATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATggcg    >  2:808485/1‑140 (MQ=255)                                                                                                                                 cgcATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATggcg    >  1:441815/1‑140 (MQ=255)                                                                                                                                 cgcATGCCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATggcg    >  1:869042/1‑140 (MQ=255)                                                                                                                                   cATGCCGAATCGTTGCTCGTATTGATAAAACAGATTCATGATCACCAAAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATggcctg  >  1:739151/1‑137 (MQ=255)                                                                                                                                      |                                                                                                                                         CTTGCGGCGACCAGCGTGCTGCGTTCATTTGCGCAAGCAGAGTTAAGCTGAATATTTCAACCAGAATGCGATGGGTGGTGTAGATAGCAATGGTGTTGGAGCCAATCACATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGC  >  NC_000913/1174244‑1174513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑