Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,189,703	A→G	V25A (GTA→GCA)	phoQ ←	sensory histidine kinase PhoQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,189,703	0	A	G	100.0%	264.4 / NA	70	V25A (GTA→GCA)	phoQ	sensory histidine kinase PhoQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/32);  total (38/32)

GCAACTTATTGTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATAT  >  NC_000913/1189566‑1189836                                                                                                                                          |                                                                                                                                      gCAACTTATTGTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGtgct                                                                                                                                     >  1:783795/1‑140 (MQ=255)        aTTGTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTg                                                                                                                              >  2:108859/1‑140 (MQ=255)               tCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCg                                                                                                                                 >  1:414703/1‑130 (MQ=255)               tCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCg                                                                                                                                 <  2:414703/130‑1 (MQ=255)                cccACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGcc                                                                                                                      <  2:979271/140‑1 (MQ=255)                    cTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACa                                                                                                                  >  2:870211/1‑140 (MQ=255)                      tCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGcc                                                                                                                      >  1:1097663/1‑134 (MQ=255)                      tCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGcc                                                                                                                      <  2:1097663/134‑1 (MQ=255)                             ggTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTa                                                                                                         <  2:35446/140‑1 (MQ=255)                               tataGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTAcc                                                                                                       >  2:1183808/1‑140 (MQ=255)                                 taGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCg                                                                                                     >  1:76953/1‑140 (MQ=255)                                      cAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCg                                                                                                >  2:379716/1‑140 (MQ=255)                                      cAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCg                                                                                                >  2:546415/1‑140 (MQ=255)                                        gATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTAcc                                                                                                       <  1:820656/131‑1 (MQ=255)                                        gATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTAcc                                                                                                       >  2:820656/1‑131 (MQ=255)                                        gATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCgag                                                                                              >  2:240365/1‑140 (MQ=255)                                        gATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCgag                                                                                              >  2:504826/1‑140 (MQ=255)                                         aTTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCgaga                                                                                             >  1:892566/1‑140 (MQ=255)                                          ttGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAGAACGTACCCGCAGCGagag                                                                                            <  1:841189/140‑1 (MQ=255)                                             ctctCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCgg                                                                                         >  2:457421/1‑140 (MQ=255)                                                 cGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGaaa                                                                                     <  2:224494/140‑1 (MQ=255)                                                 cGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGaaa                                                                                     >  2:7507/1‑140 (MQ=255)                                                         aaCAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACg                                                                             <  2:76953/140‑1 (MQ=255)                                                            aGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAACAAGACGCAg                                                                          >  2:48934/1‑140 (MQ=255)                                                            aGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAg                                                                          >  1:670660/1‑140 (MQ=255)                                                              ccGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGa                                                                               >  1:1190858/1‑133 (MQ=255)                                                              ccGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGa                                                                               <  2:1190858/133‑1 (MQ=255)                                                              ccGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTa                                                                        <  1:473003/140‑1 (MQ=255)                                                              ccGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTa                                                                        >  1:443045/1‑140 (MQ=255)                                                                gAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAAt                                                                      >  1:917598/1‑140 (MQ=255)                                                                 aaaCGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAAtt                                                                     >  1:886700/1‑140 (MQ=255)                                                                 aaaCGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAAtt                                                                     >  1:499795/1‑140 (MQ=255)                                                                 aaaCGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAAtt                                                                     >  2:883618/1‑140 (MQ=255)                                                                   aCGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAAtttt                                                                   >  1:733210/1‑140 (MQ=255)                                                                      tAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTtc                                                                >  2:899846/1‑140 (MQ=255)                                                                            tATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAgc                                                          <  2:1088714/140‑1 (MQ=255)                                                                            tATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAgc                                                          <  2:783795/140‑1 (MQ=255)                                                                              tCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCGgcgc                                                        <  2:517360/140‑1 (MQ=255)                                                                                gAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCaa                                                      <  2:290602/140‑1 (MQ=255)                                                                                  aaCTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAAtt                                                    <  1:259392/140‑1 (MQ=255)                                                                                                   aTCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGa                                                 >  2:529287/1‑126 (MQ=255)                                                                                                   aTCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGa                                                 <  2:484377/126‑1 (MQ=255)                                                                                                   aTCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGa                                                 >  1:484377/1‑126 (MQ=255)                                                                                                   aTCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGa                                                 <  1:529287/126‑1 (MQ=255)                                                                                                   aTCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTgg                                   >  2:918163/1‑140 (MQ=255)                                                                                                   aTCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTgg                                   >  1:853891/1‑140 (MQ=255)                                                                                                     cAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGcc                                 <  2:267731/140‑1 (MQ=255)                                                                                                     cAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGcc                                 >  2:1006502/1‑140 (MQ=255)                                                                                                     cAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGcc                                 >  1:281573/1‑140 (MQ=255)                                                                                                     cAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGcc                                 <  1:364416/140‑1 (MQ=255)                                                                                                        cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTTGAACAGATAGCCCTGGCcgcg                              <  2:853891/140‑1 (MQ=255)                                                                                                        cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCcgcg                              >  2:889038/1‑140 (MQ=255)                                                                                                        cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCcgcg                              >  1:787002/1‑140 (MQ=255)                                                                                                        cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCcgcg                              >  2:836605/1‑140 (MQ=255)                                                                                                        cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCcgcg                              <  2:787002/140‑1 (MQ=255)                                                                                                        cgcgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCcgcg                              >  1:802685/1‑140 (MQ=255)                                                                                                          cgACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGaa                            <  2:802685/140‑1 (MQ=255)                                                                                                                       ggCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCAc               <  1:7507/140‑1 (MQ=255)                                                                                                                       ggCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCAc               <  1:240365/140‑1 (MQ=255)                                                                                                                       ggCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCAc               >  2:192461/1‑140 (MQ=255)                                                                                                                       ggCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCAc               <  2:670660/140‑1 (MQ=255)                                                                                                                          aaGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtc            >  2:186577/1‑140 (MQ=255)                                                                                                                          aaGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActt             >  2:572004/1‑139 (MQ=255)                                                                                                                          aaGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActt             <  1:572004/139‑1 (MQ=255)                                                                                                                           aGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtct           <  1:836605/140‑1 (MQ=255)                                                                                                                           aGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtct           <  2:1161509/140‑1 (MQ=255)                                                                                                                           aGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtct           <  1:525028/140‑1 (MQ=255)                                                                                                                           aGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtct           <  1:546415/140‑1 (MQ=255)                                                                                                                           aGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtct           <  1:186577/140‑1 (MQ=255)                                                                                                                             cGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTg         >  2:629814/1‑140 (MQ=255)                                                                                                                                    aCCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGatat  <  1:883618/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GCAACTTATTGTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATAT  >  NC_000913/1189566‑1189836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑