Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,235,642	C→T	A235A (GCC→GCT)	fadR →	DNA‑binding transcriptional dual regulator FadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,235,642	0	C	T	100.0%	136.5 / NA	40	A235A (GCC→GCT)	fadR	DNA‑binding transcriptional dual regulator FadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/17);  total (23/17)

TGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCG  >  NC_000913/1235506‑1235778                                                                                                                                         |                                                                                                                                         tGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTAtt                                                                                                                                       <  1:683165/140‑1 (MQ=255)     cTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAgg                                                                                                                                   <  2:831121/140‑1 (MQ=255)     cTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAgg                                                                                                                                   <  2:1062820/140‑1 (MQ=255)       tCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAgggg                                                                                                                                 <  1:929700/140‑1 (MQ=255)              caAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAAt                                                                                                                          >  1:1002855/1‑140 (MQ=255)              caAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAAt                                                                                                                          >  1:827158/1‑140 (MQ=255)              caAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGAATTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAAt                                                                                                                          >  2:1069938/1‑140 (MQ=255)                  cTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCt                                                                                                                      <  1:1039984/140‑1 (MQ=255)                  cTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCt                                                                                                                      <  1:1069938/140‑1 (MQ=255)                     tCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTcc                                                                                                                   >  2:918257/1‑140 (MQ=255)                      cGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCg                                                                                                                  >  2:204001/1‑140 (MQ=255)                          gTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTa                                                                                                              <  1:204001/140‑1 (MQ=255)                              tgCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAaga                                                                                                          <  1:104069/140‑1 (MQ=255)                               gCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAagag                                                                                                         >  1:337204/1‑140 (MQ=255)                                   tGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCaaa                                                                                                     <  1:1124872/140‑1 (MQ=255)                                        gcgcgcACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCt                                                                                                >  2:505422/1‑140 (MQ=255)                                        gcgcgcACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCt                                                                                                >  2:114302/1‑140 (MQ=255)                                          gcgcACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCa                                                                                              >  1:82010/1‑140 (MQ=255)                                             cACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAAc                                                                                           >  2:932706/1‑140 (MQ=255)                                             cACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAAc                                                                                           >  2:9461/1‑140 (MQ=255)                                             cACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAAc                                                                                           >  2:1118307/1‑140 (MQ=255)                                                gATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAg                                                                                        <  2:386426/140‑1 (MQ=255)                                                      gtgtACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGttt                                                                                  >  2:999793/1‑140 (MQ=255)                                                         tACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGtttttt                                                                               <  1:512974/140‑1 (MQ=255)                                                                 aGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGtttt                                                                       >  2:212873/1‑140 (MQ=255)                                                                   tGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTa                                                                     >  2:547877/1‑140 (MQ=255)                                                                      gTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAg                                                                  <  1:1063222/140‑1 (MQ=255)                                                                        cGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGAt                                                                >  2:1184491/1‑140 (MQ=255)                                                                                    gagaGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTg                                                    >  2:912731/1‑140 (MQ=255)                                                                                          ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGc                                              >  1:209613/1‑140 (MQ=255)                                                                                          ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGc                                              >  1:459138/1‑140 (MQ=255)                                                                                              agaTTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACg                                          >  1:268316/1‑140 (MQ=255)                                                                                               gaTTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGt                                         >  2:133840/1‑140 (MQ=255)                                                                                                           gATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCg                             >  2:562100/1‑140 (MQ=255)                                                                                                           gATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCg                             >  1:482081/1‑140 (MQ=255)                                                                                                           gATGCAGAAAAATCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCg                             >  2:936808/1‑140 (MQ=255)                                                                                                                    acaTCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCg                    <  2:1002855/138‑1 (MQ=255)                                                                                                                    aaaTCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCg                    <  2:827158/140‑1 (MQ=255)                                                                                                                     aaTCTGCCGGGTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGt                   <  1:912731/140‑1 (MQ=255)                                                                                                                               gTGATTTAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTg         <  1:823059/140‑1 (MQ=255)                                                                                                                                     tAGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTc   <  2:125673/140‑1 (MQ=255)                                                                                                                                      aGCTATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCg  <  1:1118307/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCG  >  NC_000913/1235506‑1235778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑