Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,726	A→G	T239A (ACC→GCC)	prfA →	peptide chain release factor RF1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,726	0	A	G	100.0%	244.3 / NA	70	T239A (ACC→GCC)	prfA	peptide chain release factor RF1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/32);  total (38/32)

TCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGG  >  NC_000913/1265593‑1265861                                                                                                                                      |                                                                                                                                        tCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCg                                                                                                                                   >  2:1178286/1‑140 (MQ=255)   gTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGAt                                                                                                                                 >  1:537109/1‑140 (MQ=255)         aTACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCa                                                                                                                           <  1:812162/140‑1 (MQ=255)         aTACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCa                                                                                                                           >  2:812162/1‑140 (MQ=255)         aTACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCa                                                                                                                           <  2:703335/140‑1 (MQ=255)          tACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGc                                                                                                                            >  1:109920/1‑138 (MQ=255)          tACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGc                                                                                                                            <  2:109920/138‑1 (MQ=255)           aCTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACTGATTCGGCAAt                                                                                                                         >  1:118407/1‑140 (MQ=255)           aCTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAAt                                                                                                                         >  2:579176/1‑140 (MQ=255)           aCTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAAt                                                                                                                         >  1:986973/1‑140 (MQ=255)           aCTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAAt                                                                                                                         <  2:88835/140‑1 (MQ=255)             ttCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTc                                                                                                                       <  1:847549/140‑1 (MQ=255)               cTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCaa                                                                                                                          <  2:448204/135‑1 (MQ=255)               cTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCaa                                                                                                                          >  1:448204/1‑135 (MQ=255)               cTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGt                                                                                                                     >  2:670287/1‑140 (MQ=255)               cTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGt                                                                                                                     >  2:644071/1‑140 (MQ=255)                tGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTa                                                                                                                    >  1:572233/1‑140 (MQ=255)                  cTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTAAGCACGTTAACGCCACCGATTCGGCa                                                                                                                           <  1:1175522/131‑1 (MQ=255)                  cTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTAAGCACGTTAACGCCACCGATTCGGCa                                                                                                                           >  2:1175522/1‑131 (MQ=255)                       aCCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCa                                                                                                             >  2:414920/1‑140 (MQ=255)                       aCCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGGCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCa                                                                                                             >  2:423852/1‑140 (MQ=255)                            tGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGCTTCGGCAATTCGTATTACTCACTTGc                                                                                                        >  2:763239/1‑140 (MQ=255)                            tGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGc                                                                                                        >  1:929794/1‑140 (MQ=255)                                gTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGAc                                                                                                    <  2:1143287/140‑1 (MQ=255)                                   aTGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCgg                                                                                                 >  1:281228/1‑140 (MQ=255)                                     gCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGa                                                                                               <  1:865953/140‑1 (MQ=255)                                     gCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGa                                                                                               <  1:1178286/140‑1 (MQ=255)                                     gCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGa                                                                                               >  2:250482/1‑140 (MQ=255)                                      ccAGAACTGCCTGACGCAGACCTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGAc                                                                                                    <  1:842098/134‑1 (MQ=255)                                      ccAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGAc                                                                                                    >  2:842098/1‑134 (MQ=255)                                         gAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACTGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgt                                                                                           <  2:118407/140‑1 (MQ=255)                                          aaCTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgtt                                                                                          >  1:668125/1‑140 (MQ=255)                                          aaCTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgtt                                                                                          >  2:1165139/1‑140 (MQ=255)                                             tGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGAttgttgtt                                                                                       <  2:668125/140‑1 (MQ=255)                                               ccTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGa                                                                                     <  2:70670/140‑1 (MQ=255)                                               ccTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGa                                                                                     >  1:549046/1‑140 (MQ=255)                                               ccTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGa                                                                                     >  1:70670/1‑140 (MQ=255)                                               ccTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGa                                                                                     >  1:405200/1‑140 (MQ=255)                                                    cGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTc                                                                                >  2:31072/1‑140 (MQ=255)                                                    cGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGGGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTc                                                                                >  1:848419/1‑140 (MQ=255)                                                          aCTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACg                                                                          <  2:464734/140‑1 (MQ=255)                                                              ccGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGa                                                                         <  2:1161016/137‑1 (MQ=255)                                                              ccGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGa                                                                         >  1:1161016/1‑137 (MQ=255)                                                                  aCATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTca                                                                  <  1:1165139/140‑1 (MQ=255)                                                                   gacagaCCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTcac                                                                 <  2:1094391/135‑1 (MQ=255)                                                                   cATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTcac                                                                 <  2:572233/140‑1 (MQ=255)                                                                   cATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTcac                                                                 <  2:549046/140‑1 (MQ=255)                                                                   cATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTcac                                                                 <  1:644071/140‑1 (MQ=255)                                                                       aaCCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCacaaca                                                             <  2:992646/140‑1 (MQ=255)                                                                            agcagATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATaaaa                                                        >  2:664459/1‑140 (MQ=255)                                                                              cagATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAAc                                                      >  1:308648/1‑140 (MQ=255)                                                                              cagATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAAc                                                      <  1:664459/140‑1 (MQ=255)                                                                                gATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACaa                                                    <  1:31072/140‑1 (MQ=255)                                                                                      cGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTaa                                              <  2:308648/140‑1 (MQ=255)                                                                                           tGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCAc                                         >  1:669248/1‑140 (MQ=255)                                                                                            gATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACt                                        <  1:562444/140‑1 (MQ=255)                                                                                             aTACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAAc                                                      >  1:769647/1‑125 (MQ=255)                                                                                             aTACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAAc                                                      <  2:769647/125‑1 (MQ=255)                                                                                             aTACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACtt                                       >  2:45089/1‑140 (MQ=255)                                                                                                     cGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAg                                            >  1:1065496/1‑127 (MQ=255)                                                                                                     cGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTtct                               <  2:557899/140‑1 (MQ=255)                                                                                                     cGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTtct                               <  2:929794/140‑1 (MQ=255)                                                                                                     cGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTtct                               <  2:405200/140‑1 (MQ=255)                                                                                                     cGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTtct                               >  1:285574/1‑140 (MQ=255)                                                                                                     cGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCACGACGAACGTTCACAACATAAAAACAAAGCTAAAg                                            <  2:1065496/127‑1 (MQ=255)                                                                                                       cTCGTCAGGGGCGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCg                             >  2:827559/1‑140 (MQ=255)                                                                                                                  cGGGTGGTCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATc                  >  2:515668/1‑140 (MQ=255)                                                                                                                    ggtggtCAGCACGTTAACGCCACCGATTCGGCATTTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCa                >  2:217901/1‑140 (MQ=255)                                                                                                                    ggtggtCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCa                >  1:234094/1‑140 (MQ=255)                                                                                                                    ggtggtCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCa                <  1:250482/140‑1 (MQ=255)                                                                                                                      tggtCAGCACGTTAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACg              >  2:631952/1‑140 (MQ=255)                                                                                                                                  tAACGCCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATgg  >  1:436519/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TCGTATTCATACTTCTGCTTGTACCGTTGCGGTAATGCCAGAACTGCCTGACGCAGAACTGCCGGACATCAACCCAGCAGATTTACGCATTGATACTTTCCGCTCGTCAGGGGCGGGTGGTCAGCACGTTAACACCACCGATTCGGCAATTCGTATTACTCACTTGCCGACCGGGATTGTTGTTGAATGTCAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGG  >  NC_000913/1265593‑1265861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑