Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,275,320	A→G	G170G (GGT→GGC)	narL ←	DNA‑binding transcriptional dual regulator NarL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,275,320	0	A	G	100.0%	188.1 / NA	51	G170G (GGT→GGC)	narL	DNA‑binding transcriptional dual regulator NarL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

AATGCGCTCCTGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCTTCGCTTAATACCATTTCGCCAG  >  NC_000913/1275185‑1275456                                                                                                                                        |                                                                                                                                         aaTGCGCTCCTGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCt                                                                                                                                      <  2:144170/140‑1 (MQ=255)   tGCGCTCCTGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTgg                                                                                                                                    >  1:113467/1‑140 (MQ=255)       cTCCTGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCaa                                                                                                                                <  2:273667/140‑1 (MQ=255)       cTCCTGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCaa                                                                                                                                >  1:889390/1‑140 (MQ=255)           tGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAg                                                                                                                            <  1:81095/140‑1 (MQ=255)            gATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGc                                                                                                                           <  2:637892/140‑1 (MQ=255)                    cATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAat                                                                                                                   >  2:668061/1‑140 (MQ=255)                    cATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAat                                                                                                                   >  1:748135/1‑140 (MQ=255)                           ctgctTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCgcgc                                                                                                            >  2:975794/1‑140 (MQ=255)                           ctgctTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCgcgc                                                                                                            >  2:787779/1‑140 (MQ=255)                               ttCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTcgc                                                                                                        <  1:509514/140‑1 (MQ=255)                               ttCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTcgc                                                                                                        <  2:552363/140‑1 (MQ=255)                                tCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTcgcg                                                                                                       <  2:508690/140‑1 (MQ=255)                                 ccACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGt                                                                                                      <  2:889390/140‑1 (MQ=255)                                   aCGCGAGACTTGAGCTTCATTTTCCTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgg                                                                                                    >  1:752652/1‑140 (MQ=255)                                     gcgAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTgggg                                                                                                  <  1:276105/140‑1 (MQ=255)                                        agaCTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTg                                                                                                     <  2:198017/134‑1 (MQ=255)                                        agaCTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTg                                                                                                     >  1:198017/1‑134 (MQ=255)                                         gaCTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa                                                                                              <  1:487613/140‑1 (MQ=255)                                         gaCTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCATGAGAATATCGCGCTCGCGTGGGGTTaa                                                                                              <  1:557168/140‑1 (MQ=255)                                         gaCTTGAGCTTCATTTTCCTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTaa                                                                                              <  2:752652/140‑1 (MQ=255)                                          aCTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGt                                                                                                      >  2:358018/1‑131 (MQ=255)                                          aCTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGt                                                                                                      <  1:358018/131‑1 (MQ=255)                                                      ttttCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATcg                                                                                 >  1:152937/1‑140 (MQ=255)                                                          cttcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             <  1:290693/140‑1 (MQ=255)                                                          cttcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             <  1:668061/140‑1 (MQ=255)                                                          cttcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             <  2:748135/140‑1 (MQ=255)                                                            tcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATc                                                                                  <  2:562804/133‑1 (MQ=255)                                                            tcAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATc                                                                                  >  1:562804/1‑133 (MQ=255)                                                                   gtgCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             >  2:282198/1‑131 (MQ=255)                                                                   gtgCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             <  1:282198/131‑1 (MQ=255)                                                                    tgCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             >  2:415613/1‑130 (MQ=255)                                                                    tgCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             <  1:415613/130‑1 (MQ=255)                                                                          aCGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             <  2:796337/124‑1 (MQ=255)                                                                          aCGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCt                                                                             >  1:796337/1‑124 (MQ=255)                                                                           cGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTa                                                            <  2:113467/140‑1 (MQ=255)                                                                                     tACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGc                                                  >  1:424370/1‑140 (MQ=255)                                                                                        tgtgCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTgg                                               <  2:40919/140‑1 (MQ=255)                                                                                            cTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTggcggc                                           >  2:851982/1‑140 (MQ=255)                                                                                            cTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTggcggc                                           >  2:602555/1‑140 (MQ=255)                                                                                                  gTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAAc                                     >  2:712430/1‑140 (MQ=255)                                                                                                   tGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACa                                    <  2:402710/140‑1 (MQ=255)                                                                                                      tatCCTGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGaa                                      <  1:827056/135‑1 (MQ=255)                                                                                                      tatCCAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGaa                                      >  2:827056/1‑135 (MQ=255)                                                                                                         ccAGGCGGCGGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGtt                              >  2:500404/1‑140 (MQ=255)                                                                                                             gcggcgGGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATg                          <  1:1045623/140‑1 (MQ=255)                                                                                                                 cggGCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCTTc                      >  2:344988/1‑140 (MQ=255)                                                                                                                   ggCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCt                        <  2:426563/136‑1 (MQ=255)                                                                                                                   ggCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCt                        >  1:426563/1‑136 (MQ=255)                                                                                                                    gCAATCATCTTGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCTTCGCt                   <  1:500404/140‑1 (MQ=255)                                                                                                                              tGTTCGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCTTCGCTTAATACCAtt         >  1:239579/1‑140 (MQ=255)                                                                                                                                  cGGCAAGCCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCTAGAGCAGGCGTTAATGCTTCGCTTAATACCATTTCGc     <  2:424370/140‑1 (MQ=255)                                                                                                                                     cAAGCCCTGGGCAATCAGCTTGAGAATATCGAGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCTTCGCTTAATACCATTTCGCcag  >  2:586733/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         AATGCGCTCCTGATGCACCCATACCGCTGCTTCCACGCGAGACTTGAGCTTCATTTTCTTCAGCATGTGCTTGACGTGCACTTTTACTGTGCTTTCGGTGATATCCAGGCGGCGGGCAATCATCTTGTTCGGCAAACCCTGGGCAATCAGCTTGAGAATATCGCGCTCGCGTGGGGTTAACTGGTTAACATCGCGCTCAGTAGTGGCACGGTTAGCGCGCAAGCTGGCGGCCAGAACAGGCGTTAATGCTTCGCTTAATACCATTTCGCCAG  >  NC_000913/1275185‑1275456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑