Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,553,360	C→T	R160H (CGT→CAT)	adhP ←	ethanol dehydrogenase/alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,553,360	0	C	T	100.0%	184.2 / NA	52	R160H (CGT→CAT)	adhP	ethanol dehydrogenase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

TTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCC  >  NC_000913/1553226‑1553493                                                                                                                                       |                                                                                                                                      tttCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAAtt                                                                                                                                  >  2:1065819/1‑140 (MQ=255)  ttCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAAttt                                                                                                                                 >  1:1155294/1‑140 (MQ=255)       ttGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGAc                                                                                                                            <  1:324613/140‑1 (MQ=255)          ccAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGc                                                                                                                         >  2:481694/1‑140 (MQ=255)              ttttAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTaa                                                                                                                     <  1:796277/140‑1 (MQ=255)               tttAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAAc                                                                                                                    <  1:223741/140‑1 (MQ=255)                      gCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTg                                                                                                             <  1:481694/140‑1 (MQ=255)                        tcatcaTTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTa                                                                                                           >  2:305780/1‑140 (MQ=255)                        tcatcaTTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTa                                                                                                           >  1:1105366/1‑140 (MQ=255)                          atcatTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAgg                                                                                                         >  1:222928/1‑140 (MQ=255)                                  cATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACt                                                                                                 <  2:1155294/140‑1 (MQ=255)                                      aaTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAggtggt                                                                                                     <  1:1069146/132‑1 (MQ=255)                                      aaTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAggtggt                                                                                                     >  2:1069146/1‑132 (MQ=255)                                      aaTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTcccc                                                                                             >  1:252309/1‑139 (MQ=255)                                      aaTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCg                                                                                             <  1:31521/140‑1 (MQ=255)                                      aaTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCg                                                                                             >  1:911557/1‑140 (MQ=255)                                      aaTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCg                                                                                             >  1:1022196/1‑140 (MQ=255)                                       aTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGc                                                                                            <  1:305780/140‑1 (MQ=255)                                        tGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGc                                                                                            <  2:1024254/139‑1 (MQ=255)                                        tGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGc                                                                                            >  1:1024254/1‑139 (MQ=255)                                           cGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAg                                                                                        >  1:369186/1‑140 (MQ=255)                                                    tGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAAtgctg                                                                               >  1:906258/1‑140 (MQ=255)                                                           aaaGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGgccg                                                                        >  2:1145264/1‑140 (MQ=255)                                                               aCATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGgccgccgc                                                                    <  2:222928/140‑1 (MQ=255)                                                               aCATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGgccgccgc                                                                    <  2:1105366/140‑1 (MQ=255)                                                               aCATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGgccgccgc                                                                    <  1:954854/140‑1 (MQ=255)                                                               aCATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGgccgccgc                                                                    <  1:17917/140‑1 (MQ=255)                                                                 aTTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGccgccgccg                                                                  >  2:532062/1‑140 (MQ=255)                                                                   tcttcGGGTATTGCAGGGGGAGGTTTCCCAAGCCCCCAAGACCGTAGATAGCAAACCCCCGCCCCGGAAGGAATTTTGGCCACTTTACCGCTTTTTAAGGGGGGGCTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAg                                                                <  2:252309/140‑1 (MQ=255)                                                                      tcGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTcc                                                             <  2:369186/140‑1 (MQ=255)                                                                      tcGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTcc                                                             >  1:500293/1‑140 (MQ=255)                                                                                    gCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCAc                                               <  2:906258/140‑1 (MQ=255)                                                                                        ggTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACtttt                                           >  1:1040282/1‑140 (MQ=255)                                                                                        ggTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACtttt                                           >  2:150938/1‑140 (MQ=255)                                                                                          ttACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTAc                                         >  1:569518/1‑140 (MQ=255)                                                                                                    cGCCAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTATAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCg                               >  2:1075045/1‑140 (MQ=255)                                                                                                      ccAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGc                             >  2:264675/1‑140 (MQ=255)                                                                                                      ccAAGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGc                             >  1:602083/1‑140 (MQ=255)                                                                                                         aGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGAc                          <  1:150938/140‑1 (MQ=255)                                                                                                         aGACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGAc                          <  2:500293/140‑1 (MQ=255)                                                                                                          gACCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGAcc                         <  1:945239/140‑1 (MQ=255)                                                                                                           aCCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCa                        <  1:532062/140‑1 (MQ=255)                                                                                                           aCCGTAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCa                        <  2:602083/140‑1 (MQ=255)                                                                                                               tAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCa                        <  1:600118/136‑1 (MQ=255)                                                                                                               tAGATAGCAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCa                        >  2:600118/1‑136 (MQ=255)                                                                                                                      cAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACTGCGTAATCGGCGACCACGATGCActct             >  2:54029/1‑140 (MQ=255)                                                                                                                      cAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCa                 <  1:447538/136‑1 (MQ=255)                                                                                                                      cAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCa                 >  2:447538/1‑136 (MQ=255)                                                                                                                      cAATCCACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCActct             >  1:681952/1‑140 (MQ=255)                                                                                                                          ccACTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTccg         >  2:953823/1‑140 (MQ=255)                                                                                                                             cTGCCCTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGGGTAATCGGCGACCACGATGCACTCTTccgcca      >  2:76274/1‑140 (MQ=255)                                                                                                                                cccTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTccgccatcc   >  1:630100/1‑140 (MQ=255)                                                                                                                                 ccTGGATGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTccgccatccc  <  1:1065819/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCC  >  NC_000913/1553226‑1553493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑