Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,700,737	T→C	G461G (GGT→GGC)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,737	0	T	C	100.0%	248.9 / NA	67	G461G (GGT→GGC)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/42);  total (25/42)

CGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTG  >  NC_000913/1700600‑1700872                                                                                                                                          |                                                                                                                                        cGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGgcgc                                                                                                                                       >  2:605983/1‑140 (MQ=255)  gTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGcc                                                                                                                                      >  1:350877/1‑140 (MQ=255)   tttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCg                                                                                                                                     >  1:455973/1‑140 (MQ=255)   tttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCg                                                                                                                                     <  2:438626/140‑1 (MQ=255)    ttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGa                                                                                                                                    <  2:693759/140‑1 (MQ=255)      cGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACa                                                                                                                                  >  1:138191/1‑140 (MQ=255)      cGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACa                                                                                                                                  >  2:174036/1‑140 (MQ=255)         tATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAata                                                                                                                               >  1:603641/1‑140 (MQ=255)              cccGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTc                                                                                                                          >  1:141706/1‑140 (MQ=255)                  cTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGcc                                                                                                                      <  2:753612/140‑1 (MQ=255)                   ttCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCt                                                                                                                     >  1:573691/1‑140 (MQ=255)                     cAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTc                                                                                                                    >  2:502072/1‑139 (MQ=255)                     cAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTc                                                                                                                    <  1:502072/139‑1 (MQ=255)                       aTCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGAt                                                                                                                 <  1:175781/140‑1 (MQ=255)                       aTCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGAt                                                                                                                 <  2:146032/140‑1 (MQ=255)                        tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATa                                                                                                                <  2:603641/140‑1 (MQ=255)                         cTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATaa                                                                                                               <  1:616047/140‑1 (MQ=255)                            aaaaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTg                                                                                                            <  2:172342/140‑1 (MQ=255)                             aaaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGc                                                                                                           >  1:183820/1‑140 (MQ=255)                                    ggggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTAccc                                                                                                    >  1:964611/1‑140 (MQ=255)                                      ggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGt                                                                                                  >  2:577878/1‑140 (MQ=255)                                                cGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGt                                                                                        <  2:786574/140‑1 (MQ=255)                                                cGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGt                                                                                        >  2:662507/1‑140 (MQ=255)                                                cGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGt                                                                                        <  2:699268/140‑1 (MQ=255)                                                  aaGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCt                                                                                      <  2:964611/140‑1 (MQ=255)                                                   aGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtg                                                                                     <  1:159071/140‑1 (MQ=255)                                                     ttGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgtg                                                                                   >  1:455311/1‑140 (MQ=255)                                                          aGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGa                                                                              >  2:956998/1‑140 (MQ=255)                                                           gCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGat                                                                             <  1:139457/140‑1 (MQ=255)                                                            cTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGata                                                                            <  1:1001793/140‑1 (MQ=255)                                                              cAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATg                                                                          <  1:431870/140‑1 (MQ=255)                                                              cAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATg                                                                          <  2:455311/140‑1 (MQ=255)                                                              cAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATg                                                                          <  2:1157801/140‑1 (MQ=255)                                                                 tCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCg                                                                       >  2:247622/1‑140 (MQ=255)                                                                 tCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCg                                                                       <  2:141706/140‑1 (MQ=255)                                                                      aaaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGt                                                                  >  1:1135236/1‑140 (MQ=255)                                                                       aaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGtt                                                                 <  2:484756/140‑1 (MQ=255)                                                                           cGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAAtg                                                             >  2:520469/1‑140 (MQ=255)                                                                             ttGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAAtgtg                                                           <  2:1135236/140‑1 (MQ=255)                                                                              tGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGc                                                          <  1:956998/140‑1 (MQ=255)                                                                               gCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCa                                                         >  1:924633/1‑140 (MQ=255)                                                                                 cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAgg                                                       <  1:245372/140‑1 (MQ=255)                                                                                 cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAgg                                                       <  1:805927/140‑1 (MQ=255)                                                                                 cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAgg                                                       <  1:520469/140‑1 (MQ=255)                                                                                   gTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCa                                                     <  2:138191/140‑1 (MQ=255)                                                                                   gTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCa                                                     <  2:931907/140‑1 (MQ=255)                                                                                    tGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCAc                                                    <  1:662507/140‑1 (MQ=255)                                                                                        ccGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGaa                                                >  1:728964/1‑140 (MQ=255)                                                                                         cGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAg                                               <  2:514949/140‑1 (MQ=255)                                                                                              aaaTCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACaa                                          >  1:881967/1‑140 (MQ=255)                                                                                                  cAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCgg                                      <  2:924633/140‑1 (MQ=255)                                                                                                     gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAg                                               <  2:270123/128‑1 (MQ=255)                                                                                                     gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAg                                               >  1:270123/1‑128 (MQ=255)                                                                                                     gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCa                                   >  2:1133054/1‑140 (MQ=255)                                                                                                      ttACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCTTTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCaa                                  >  2:945841/1‑140 (MQ=255)                                                                                                       tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCTTTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAAt                                 <  1:945841/140‑1 (MQ=255)                                                                                                       tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAAt                                 <  1:605983/140‑1 (MQ=255)                                                                                                          aaCGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTgg                              <  2:881967/140‑1 (MQ=255)                                                                                                          aaCGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTgg                              <  1:1133054/140‑1 (MQ=255)                                                                                                           aCGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGc                             <  2:728964/140‑1 (MQ=255)                                                                                                            cGTTCCTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg                            <  2:183820/140‑1 (MQ=255)                                                                                                                ccTGCAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCgtagt                        <  2:244095/140‑1 (MQ=255)                                                                                                                    cAATCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACaa                    >  1:289235/1‑140 (MQ=255)                                                                                                                     aaTCCTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAAc                   <  2:810987/140‑1 (MQ=255)                                                                                                                         cTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg                            <  1:838951/127‑1 (MQ=255)                                                                                                                         cTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg                            >  2:838951/1‑127 (MQ=255)                                                                                                                         cTCGAAGCATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTaa               <  1:1153332/140‑1 (MQ=255)                                                                                                                                cATTAGGCGGCGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACAt        <  1:247622/140‑1 (MQ=255)                                                                                                                                     ggcggcGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCt   >  1:513620/1‑140 (MQ=255)                                                                                                                                      gcggcgCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTg  <  1:577878/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTG  >  NC_000913/1700600‑1700872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑