Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,816,368	T→C	intergenic (+240/+18)	katE → / ← chbG	catalase II/chitin disaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,816,368	0	T	C	100.0%	189.7 / NA	50	intergenic (+240/+18)	katE/chbG	catalase II/chitin disaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/29);  total (21/29)

AATATATTGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCATCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGG  >  NC_000913/1816235‑1816503                                                                                                                                      |                                                                                                                                        aaTATATTGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCa                                                                                                                                   <  1:600247/140‑1 (MQ=255) aaTATATTGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCa                                                                                                                                   <  1:392144/140‑1 (MQ=255)     tatTGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACa                                                                                                                               <  2:16685/140‑1 (MQ=255)       ttGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATc                                                                                                                             <  2:508252/140‑1 (MQ=255)             gAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACt                                                                                                                       >  1:742200/1‑140 (MQ=255)             gAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACt                                                                                                                       >  1:994386/1‑140 (MQ=255)               aaCACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTa                                                                                                                     >  1:53383/1‑140 (MQ=255)                  acGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTacac                                                                                                                  <  1:736717/140‑1 (MQ=255)                      aGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATcc                                                                                                              <  1:1027681/140‑1 (MQ=255)                            gATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATaa                                                                                                        >  1:668325/1‑140 (MQ=255)                             aTAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAAc                                                                                                       >  1:311736/1‑140 (MQ=255)                              tAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACt                                                                                                      >  1:129544/1‑140 (MQ=255)                                 gcgaagcgCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTccc                                                                                                   <  1:277191/140‑1 (MQ=255)                                  cgaagcgCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTcccc                                                                                                  >  1:525542/1‑140 (MQ=255)                                  cgaagcgCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTcccc                                                                                                  <  1:589980/140‑1 (MQ=255)                                       cgcATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCg                                                                                             >  2:654919/1‑140 (MQ=255)                                             aGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAAcc                                                                                       <  1:160425/140‑1 (MQ=255)                                                   ttttGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTg                                                                                 >  2:143039/1‑140 (MQ=255)                                                           ttGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAg                                                                         <  1:654919/140‑1 (MQ=255)                                                             gTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCa                                                                       <  1:221584/140‑1 (MQ=255)                                                             gTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCa                                                                       <  1:466650/140‑1 (MQ=255)                                                                     tctcAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCaa                                                               <  1:83558/140‑1 (MQ=255)                                                                       tcAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAacg                                                             >  1:1064931/1‑140 (MQ=255)                                                                       tcAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAacg                                                             >  1:336404/1‑140 (MQ=255)                                                                          aGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAacgacg                                                          <  2:53383/140‑1 (MQ=255)                                                                               cgcAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGAt                                                     >  2:789500/1‑140 (MQ=255)                                                                               cgcAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGAt                                                     >  2:245809/1‑140 (MQ=255)                                                                               cgcAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGAt                                                     >  1:28048/1‑140 (MQ=255)                                                                                gcAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATg                                                    <  2:336404/140‑1 (MQ=255)                                                                                gcAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATg                                                    <  2:311736/140‑1 (MQ=255)                                                                                 cAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGt                                                   >  1:69254/1‑140 (MQ=255)                                                                                         ccgccTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACAt                                           >  1:873236/1‑140 (MQ=255)                                                                                              tttGTAGGAATTAATCGCCGGATGCAAGGTACACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGc                                      <  2:742200/140‑1 (MQ=255)                                                                                                tGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTc                                    >  1:543714/1‑140 (MQ=255)                                                                                                    ggAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGtt                                <  2:994386/140‑1 (MQ=255)                                                                                                    ggAATTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGtt                                <  1:789500/140‑1 (MQ=255)                                                                                                      aaTTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCCGCTCCGTTaa                              <  2:129544/140‑1 (MQ=255)                                                                                                      aaTTAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTaa                              >  1:775921/1‑140 (MQ=255)                                                                                                        ttAATCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGc                            <  1:687613/140‑1 (MQ=255)                                                                                                           aTCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAg                                       >  2:1170055/1‑126 (MQ=255)                                                                                                           aTCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAg                                       <  1:1170055/126‑1 (MQ=255)                                                                                                           aTCGCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTg                         >  1:651654/1‑140 (MQ=255)                                                                                                              gCCGGATGCAAGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGa                      <  1:743069/140‑1 (MQ=255)                                                                                                                       aaGGTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAg             <  2:525542/140‑1 (MQ=255)                                                                                                                         ggTTCACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCa           <  1:447803/140‑1 (MQ=255)                                                                                                                             cACGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCActct       >  2:1046882/1‑140 (MQ=255)                                                                                                                              aCGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTg      <  1:245809/140‑1 (MQ=255)                                                                                                                              aCGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTg      <  1:143039/140‑1 (MQ=255)                                                                                                                              aCGCCGCACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTg      <  2:668325/140‑1 (MQ=255)                                                                                                                                  cgcACCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACgg  <  2:69254/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AATATATTGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGCAGTTTTGCGTTTGTCAGCAGTCTCAAGCGGCGCAGTTACGCCGCCTTTGTAGGAATTAATCGCCGGATGCAAGGTTCACGCCGCATCTGGCAAACATCCTCACTTACACATCCCGATAACTCCCCAACCGATAACCACGCTGAGCGATAGCACCTTTCAACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGG  >  NC_000913/1816235‑1816503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑