Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,002,150	T→C	N486S (AAC→AGC)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,002,150	0	T	C	100.0%	225.4 / NA	60	N486S (AAC→AGC)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/31);  total (29/31)

TCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTC  >  NC_000913/2002016‑2002281                                                                                                                                       |                                                                                                                                    tCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCt                                                                                                                                <  1:709553/140‑1 (MQ=255)     tAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTg                                                                                                                            <  2:180936/140‑1 (MQ=255)             aaTTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACg                                                                                                                    >  1:168577/1‑140 (MQ=255)             aaTTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACg                                                                                                                    <  2:480499/140‑1 (MQ=255)              aTTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACgg                                                                                                                   <  1:74166/140‑1 (MQ=255)                        tAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCCTTTGCCAACACGGAGTTACCGGc                                                                                                         <  2:1119999/140‑1 (MQ=255)                          ggCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCct                                                                                                       <  1:857886/140‑1 (MQ=255)                               gATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTgg                                                                                                  >  1:30554/1‑140 (MQ=255)                                aTAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGga                                                                                                 >  1:190701/1‑140 (MQ=255)                                     cgcagcgcaTCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCgg                                                                                                          >  1:794925/1‑126 (MQ=255)                                     cgcagcgcaTCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCgg                                                                                                          <  2:794925/126‑1 (MQ=255)                                          cgcaTCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTgcgc                                                                                       >  2:1182466/1‑140 (MQ=255)                                          cgcaTCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTgcgc                                                                                       >  2:143598/1‑140 (MQ=255)                                          cgcaTCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTgcgc                                                                                       >  1:725945/1‑140 (MQ=255)                                          cgcaTCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTgcgc                                                                                       >  1:921917/1‑140 (MQ=255)                                               cAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCg                                                                                  >  2:617140/1‑140 (MQ=255)                                               cAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCg                                                                                  >  1:1086290/1‑140 (MQ=255)                                                 ggCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGAc                                                                                <  1:955435/140‑1 (MQ=255)                                                 ggCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGAc                                                                                <  2:725945/140‑1 (MQ=255)                                                       ttGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTg                                                                          <  2:954651/140‑1 (MQ=255)                                                              tGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACtt                                                                   >  1:336394/1‑140 (MQ=255)                                                               gCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTg                                                                          >  1:403198/1‑132 (MQ=255)                                                               gCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTg                                                                          <  2:403198/132‑1 (MQ=255)                                                                           aGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTc                                                      >  2:635093/1‑140 (MQ=255)                                                                            gTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCg                                                     <  1:635093/140‑1 (MQ=255)                                                                            gTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCg                                                     <  2:921917/140‑1 (MQ=255)                                                                            gTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCg                                                     <  1:1182466/140‑1 (MQ=255)                                                                                 tCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTc                                                <  2:247524/140‑1 (MQ=255)                                                                                 tCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTc                                                >  1:219650/1‑140 (MQ=255)                                                                                              aTTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCg                                                     <  1:217778/122‑1 (MQ=255)                                                                                              aTTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCg                                                     >  2:217778/1‑122 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  >  1:983405/1‑140 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  >  1:1027820/1‑140 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  >  1:861738/1‑140 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  <  1:348413/140‑1 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  >  1:1173242/1‑140 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  <  1:1127059/140‑1 (MQ=255)                                                                                               ttAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  >  1:1071449/1‑140 (MQ=255)                                                                                                        cagcagAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAg                         >  1:651927/1‑140 (MQ=255)                                                                                                        cagcagAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAg                         >  2:104988/1‑140 (MQ=255)                                                                                                         agcagAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGa                        <  2:983405/140‑1 (MQ=255)                                                                                                         agcagAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGa                        <  2:1027820/140‑1 (MQ=255)                                                                                                         agcagAGACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGa                        <  1:143598/140‑1 (MQ=255)                                                                                                          gcagAGACAGAACCTGCTGCGGTACCTGGCTAGCTGTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGAc                       <  1:104988/140‑1 (MQ=255)                                                                                                            agagACAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAg                     <  2:168577/140‑1 (MQ=255)                                                                                                             gatacacAAGCTGCTGTGGAACCTGGCCAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAgg                    >  2:810875/4‑140 (MQ=255)                                                                                                             gagaCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  >  1:774359/1‑126 (MQ=255)                                                                                                             gagaCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACt                                  <  2:774359/126‑1 (MQ=255)                                                                                                             gagaCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAgg                    >  1:937134/1‑140 (MQ=255)                                                                                                              agaCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGa                        >  1:215149/1‑135 (MQ=255)                                                                                                              agaCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGa                        <  2:215149/135‑1 (MQ=255)                                                                                                              agaCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGt                   <  1:288269/140‑1 (MQ=255)                                                                                                                aCAGAACCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTg                 >  2:318904/1‑140 (MQ=255)                                                                                                                     aCCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAgt            >  2:745045/1‑140 (MQ=255)                                                                                                                     aCCTGCTGCGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAgt            >  2:860782/1‑140 (MQ=255)                                                                                                                          ctgcGGTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGgtgt       >  2:282113/1‑140 (MQ=255)                                                                                                                              ggTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTgttgtt   <  2:937134/140‑1 (MQ=255)                                                                                                                               gTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTc  <  2:30554/140‑1 (MQ=255)                                                                                                                               gTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTc  <  1:860782/140‑1 (MQ=255)                                                                                                                               gTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTc  <  1:745045/140‑1 (MQ=255)                                                                                                                               gTACCTGGCTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTc  <  1:318904/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTC  >  NC_000913/2002016‑2002281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑