Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,016,617 T→C L22L (TTG→CTG)  fliI → flagellum‑specific ATP synthase FliI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,016,6170TC100.0% 197.6 / NA 52L22L (TTG→CTG) fliIflagellum‑specific ATP synthase FliI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/28);  total (24/28)

GATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGAAACGCACGAAGTAGAAA  >  NC_000913/2016486‑2016752
                                                                                                                                   |                                                                                                                                       
gATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCg                                                                                                                                 <  1:703964/140‑1 (MQ=255)
    tcGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTAc                                                                                                                             <  1:403277/140‑1 (MQ=255)
        cGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCg                                                                                                                         >  1:834765/1‑140 (MQ=255)
            aGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTAc                                                                                                                     >  1:470920/1‑140 (MQ=255)
              tgtCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACgg                                                                                                                   >  2:632824/1‑140 (MQ=255)
              tgtCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACgg                                                                                                                   <  2:470920/140‑1 (MQ=255)
                   ccACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGAt                                                                                                              <  1:632824/140‑1 (MQ=255)
                        cGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACg                                                                                                                    <  2:73075/129‑1 (MQ=255)
                        cGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACg                                                                                                                    >  1:73075/1‑129 (MQ=255)
                        cGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAAcc                                                                                                         <  1:121839/140‑1 (MQ=255)
                        cGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAAcc                                                                                                         >  2:404995/1‑140 (MQ=255)
                          cTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCcg                                                                                                       >  2:228474/1‑140 (MQ=255)
                          cTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCcg                                                                                                       <  1:404995/140‑1 (MQ=255)
                             gCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCgcgc                                                                                                    <  1:990832/140‑1 (MQ=255)
                             gCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCgcgc                                                                                                    <  2:184633/140‑1 (MQ=255)
                                    ctctGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCggg                                                                                             >  2:150873/1‑140 (MQ=255)
                                       tGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTg                                                                                          >  1:335980/1‑140 (MQ=255)
                                       tGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTg                                                                                          >  1:501443/1‑140 (MQ=255)
                                            tCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCt                                                                                           >  1:204055/1‑134 (MQ=255)
                                            tCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCt                                                                                           <  2:204055/134‑1 (MQ=255)
                                              tGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCgcgc                                                                                                    >  2:777069/1‑123 (MQ=255)
                                              tGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCgcgc                                                                                                    <  1:777069/123‑1 (MQ=255)
                                               ggCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGa                                                                                  <  2:335980/140‑1 (MQ=255)
                                                 cagcaCCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAg                                                                                >  2:515912/1‑140 (MQ=255)
                                                  agcaCCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGc                                                                               <  1:169964/140‑1 (MQ=255)
                                                    caCCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCa                                                                             <  2:489665/140‑1 (MQ=255)
                                                     aCCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCAc                                                                            <  1:150873/140‑1 (MQ=255)
                                                     aCCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCAc                                                                            <  2:862383/140‑1 (MQ=255)
                                                     aCCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCAc                                                                            <  2:450191/140‑1 (MQ=255)
                                                      ccAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCAcc                                                                           >  2:280909/1‑140 (MQ=255)
                                                       cAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCAc                                                                            <  2:731736/138‑1 (MQ=255)
                                                       cAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCAc                                                                            >  1:731736/1‑138 (MQ=255)
                                                           aGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGAtt                                                                      <  1:228474/140‑1 (MQ=255)
                                                               gtgtAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACaa                                                                  >  1:1127490/1‑140 (MQ=255)
                                                               gtgtAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACaa                                                                  >  2:830089/1‑140 (MQ=255)
                                                               gtgtAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACaa                                                                  >  1:579975/1‑140 (MQ=255)
                                                                tgtAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAAt                                                                 >  2:356018/1‑140 (MQ=255)
                                                                  tAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTg                                                               <  1:546860/140‑1 (MQ=255)
                                                                      gACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGc                                                           <  1:515912/140‑1 (MQ=255)
                                                                       aCCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCt                                                          <  1:627824/140‑1 (MQ=255)
                                                                         cACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCg                                                        >  1:669354/1‑140 (MQ=255)
                                                                           cgcgCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGgc                                                      >  1:562919/1‑140 (MQ=255)
                                                                             cgcCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGgcgc                                                    <  2:341774/140‑1 (MQ=255)
                                                                                        cTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCa                                         >  2:715615/1‑140 (MQ=255)
                                                                                        cTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCa                                         >  2:827047/1‑140 (MQ=255)
                                                                                           gCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTg                                      <  1:827047/140‑1 (MQ=255)
                                                                                                     cTGGATAACTTTGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGaa                            >  2:666665/1‑140 (MQ=255)
                                                                                                              tttGAAGCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGa                   <  2:501443/140‑1 (MQ=255)
                                                                                                                    gCCAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTTGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGAAACGCa             <  2:521977/140‑1 (MQ=255)
                                                                                                                      cAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGAAACGCACg           <  1:717193/140‑1 (MQ=255)
                                                                                                                      cAAAATGGCGCAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGAAACGCACg           <  2:834765/140‑1 (MQ=255)
                                                                                                                               gcAGCTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGAAACGCACGAAGTAGaaa  >  1:237627/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTACGTCGCTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCATTGAGCGCCAGAACGGCAGCGAAACGCACGAAGTAGAAA  >  NC_000913/2016486‑2016752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑