Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,028,703	A→G	L222L (TTG→CTG)	yedI ←	conserved inner membrane protein YedI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,028,703	0	A	G	100.0%	184.0 / NA	50	L222L (TTG→CTG)	yedI	conserved inner membrane protein YedI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

TGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCAC  >  NC_000913/2028570‑2028838                                                                                                                                      |                                                                                                                                        tgctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGATGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAAccc                                                                                                                                   >  1:161304/1‑140 (MQ=255) tgctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAAccc                                                                                                                                   >  1:844799/1‑140 (MQ=255) tgctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAAccc                                                                                                                                   >  1:129960/1‑140 (MQ=255) tgctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAAccc                                                                                                                                   >  2:694569/1‑140 (MQ=255) tgctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAAccc                                                                                                                                   >  2:381068/1‑140 (MQ=255) tgctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAAccc                                                                                                                                   <  1:52180/140‑1 (MQ=255)  gctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCt                                                                                                                                  <  2:337143/140‑1 (MQ=255)   ctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCtt                                                                                                                                 <  1:987052/140‑1 (MQ=255)   ctgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCtt                                                                                                                                 <  1:36843/140‑1 (MQ=255)    tgcCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCttt                                                                                                                                >  2:246013/1‑140 (MQ=255)       ccGGCGAAATGTTCAATCGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATCACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTAcc                                                                                                                             <  2:1071535/140‑1 (MQ=255)         ggCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGATGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTa                                                                                                                           <  2:161304/140‑1 (MQ=255)           cGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTa                                                                                                                           >  2:129512/1‑138 (MQ=255)           cGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTa                                                                                                                           <  1:129512/138‑1 (MQ=255)                       tGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAgc                                                                                                             >  2:1100845/1‑140 (MQ=255)                          cGTGATGCAGCGGCGCAATTCCTTGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAgcgcg                                                                                                          <  1:851334/140‑1 (MQ=255)                          cGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTa                                                                                                                           >  1:1057416/1‑123 (MQ=255)                          cGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTa                                                                                                                           <  2:1057416/123‑1 (MQ=255)                                    cggcgCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAttttt                                                                                                >  2:501441/1‑140 (MQ=255)                                       cgcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCg                                                                                             <  1:140427/140‑1 (MQ=255)                                                   tACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATTcc                                                                                 >  2:746561/1‑140 (MQ=255)                                                      cacaATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCa                                                                              <  2:844799/140‑1 (MQ=255)                                                      cacaATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCa                                                                              >  2:102019/1‑140 (MQ=255)                                                        caATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCgcc                                                                                           <  1:952297/125‑1 (MQ=255)                                                        caATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCgcc                                                                                           >  2:952297/1‑125 (MQ=255)                                                        caATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAcccc                                                                               >  2:634728/1‑137 (MQ=255)                                                        caATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAcccc                                                                               <  1:634728/137‑1 (MQ=255)                                                               aCCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCaa                                                                     >  2:563432/1‑140 (MQ=255)                                                                ccgccgACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAAt                                                                    >  2:500085/1‑140 (MQ=255)                                                                     gacgaGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTaa                                                               >  2:1007999/1‑140 (MQ=255)                                                                        gaGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTATCCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGa                                                            <  1:1039219/140‑1 (MQ=255)                                                                        gaGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGa                                                            <  1:1100845/140‑1 (MQ=255)                                                                           gAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGtcatc                                                                       >  2:136942/1‑126 (MQ=255)                                                                           gAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGtcatc                                                                       <  1:136942/126‑1 (MQ=255)                                                                            aaCATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAAc                                                        <  1:381068/140‑1 (MQ=255)                                                                            aaCATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAAc                                                        <  1:224735/140‑1 (MQ=255)                                                                            aaCATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAAc                                                        <  1:246013/140‑1 (MQ=255)                                                                                 cGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAAc                                                        >  1:599181/1‑135 (MQ=255)                                                                                 cGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAAc                                                        <  2:599181/135‑1 (MQ=255)                                                                                  gCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATTCCCCAGGTCATCAATCTTAACGATAACCCCTAc                                                  <  1:746561/140‑1 (MQ=255)                                                                                  gCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGa                                                            >  2:1189754/1‑130 (MQ=255)                                                                                  gCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGa                                                            <  1:1189754/130‑1 (MQ=255)                                                                                           ccGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTa                                         <  1:102019/140‑1 (MQ=255)                                                                                                   cGATAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCa                                 <  1:500085/140‑1 (MQ=255)                                                                                                     aTAACGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACg                               >  2:997659/1‑140 (MQ=255)                                                                                                         cGCTTTCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCa                           >  2:607925/1‑140 (MQ=255)                                                                                                            tttCATCAGCCAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTa                        >  2:191079/1‑140 (MQ=255)                                                                                                                     ccAGGGCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGa               <  1:563432/140‑1 (MQ=255)                                                                                                                        gggCGCGATAATCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGc            >  2:359276/1‑140 (MQ=255)                                                                                                                                  aTCAGTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcac  <  1:607925/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCAC  >  NC_000913/2028570‑2028838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑