Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,095,429	G→A	G102D (GGC→GAC)	hisA →	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,095,429	0	G	A	100.0%	118.7 / NA	36	G102D (GGC→GAC)	hisA	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/19);  total (17/19)

CCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCT  >  NC_000913/2095293‑2095557                                                                                                                                         |                                                                                                                                 ccGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTc                                                                                                                               >  2:539253/1‑140 (MQ=255)         aCGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCgg                                                                                                                       >  2:852629/1‑140 (MQ=255)          cGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGt                                                                                                                      <  2:585948/140‑1 (MQ=255)            tCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGa                                                                                                                    <  2:296559/140‑1 (MQ=255)              aaaTCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGaaa                                                                                                                  >  1:835923/1‑140 (MQ=255)               aaTCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAAt                                                                                                                 >  1:372583/1‑140 (MQ=255)               aaTCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAAt                                                                                                                 <  2:453964/140‑1 (MQ=255)                  cccGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATcac                                                                                                              <  2:36850/140‑1 (MQ=255)                  cccGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATcac                                                                                                              <  2:372583/140‑1 (MQ=255)                   ccGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATcaca                                                                                                             <  1:539253/140‑1 (MQ=255)                   ccGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATcaca                                                                                                             <  1:852629/140‑1 (MQ=255)                     gCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAg                                                                                                           >  2:611532/1‑140 (MQ=255)                        gATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGata                                                                                                        <  1:496617/140‑1 (MQ=255)                         aTTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGatat                                                                                                       >  2:810650/1‑140 (MQ=255)                             aaaCCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTg                                                                                                   <  1:405578/140‑1 (MQ=255)                                  cTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAgg                                                                                              <  2:262402/140‑1 (MQ=255)                                     gTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGat                                                                                                         >  1:757210/1‑126 (MQ=255)                                     gTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGat                                                                                                         <  2:757210/126‑1 (MQ=255)                                            gCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGaa                                                                                    <  2:29833/140‑1 (MQ=255)                                                      tCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTg                                                                          >  2:626147/1‑140 (MQ=255)                                                      tCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTg                                                                          >  2:196239/1‑140 (MQ=255)                                                          gTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGa                                                                      <  1:166287/140‑1 (MQ=255)                                                          gTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGa                                                                      >  1:377724/1‑140 (MQ=255)                                                          gTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGa                                                                      <  1:196239/140‑1 (MQ=255)                                                              aGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGcc                                                                  >  2:779002/1‑140 (MQ=255)                                                                          gcgtgcgtAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGgcg                                                      >  1:615805/1‑140 (MQ=255)                                                                                         aagaTGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTAtt                                       >  2:1194721/1‑140 (MQ=255)                                                                                                 gcggcgTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCa                               >  2:359070/1‑140 (MQ=255)                                                                                                       ttACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGcaa                         <  2:835923/140‑1 (MQ=255)                                                                                                           tGGAAGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAg                     <  2:106656/140‑1 (MQ=255)                                                                                                               aGCGGGCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAgg                 >  2:1114888/1‑140 (MQ=255)                                                                                                                  gggCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTgg              <  1:611532/140‑1 (MQ=255)                                                                                                                   ggCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGc             >  1:45109/1‑140 (MQ=255)                                                                                                                   ggCGTTGCGCGCGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGc             >  1:290836/1‑140 (MQ=255)                                                                                                                         gcgcgcGTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAg       <  1:359070/140‑1 (MQ=255)                                                                                                                           gcgcgTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCg     >  1:196329/1‑140 (MQ=255)                                                                                                                           gcgcgTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCg     <  1:1114888/140‑1 (MQ=255)                                                                                                                              cgTAGTGGTCGACTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCt  >  2:705814/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 CCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATCACAAGATATGGTGAAAGGCTGGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCT  >  NC_000913/2095293‑2095557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑