Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,261	G→A	Q384* (CAG→TAG)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,261	0	G	A	100.0%	264.2 / NA	75	Q384* (CAG→TAG)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/39);  total (36/39)

CCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGT  >  NC_000913/2260128‑2260397                                                                                                                                      |                                                                                                                                         ccATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCa                                                                                                                                    <  2:1029064/140‑1 (MQ=255) ccATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCa                                                                                                                                    <  1:705328/140‑1 (MQ=255)          tGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAg                                                                                                                           >  1:927061/1‑140 (MQ=255)          tGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAg                                                                                                                           <  2:92182/140‑1 (MQ=255)              tACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCt                                                                                                                       >  2:1201817/1‑140 (MQ=255)                 tCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCg                                                                                                                    >  2:570837/1‑140 (MQ=255)                     cagACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGaa                                                                                                                >  2:1153594/1‑140 (MQ=255)                        aCCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGc                                                                                                             >  1:841889/1‑140 (MQ=255)                        aCCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGc                                                                                                             >  1:810256/1‑140 (MQ=255)                              acCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCaa                                                                                                       <  2:810081/140‑1 (MQ=255)                                  aaTGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTgg                                                                                                   >  2:340893/1‑140 (MQ=255)                                  aaTGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTgg                                                                                                   >  2:65921/1‑140 (MQ=255)                                    tGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGtt                                                                                                 >  1:45180/1‑140 (MQ=255)                                     gCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGttt                                                                                                <  2:432945/140‑1 (MQ=255)                                        tACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTAcc                                                                                             >  2:195232/1‑140 (MQ=255)                                         aCGCTGCTTTGTTTACCGGACGGCACATGTCAGTACACATCATGCCACCGAGGATCGCCCTCTGCAGATCCGCATTCGCAGTCCCCAGGGTTTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg                                                                                            <  2:1149014/140‑1 (MQ=255)                                         aCGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg                                                                                            <  1:156668/140‑1 (MQ=255)                                         aCGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg                                                                                            <  2:237231/140‑1 (MQ=255)                                         aCGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg                                                                                            <  1:730100/140‑1 (MQ=255)                                         aCGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg                                                                                            <  1:1107644/140‑1 (MQ=255)                                         aCGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCg                                                                                            <  2:1184252/140‑1 (MQ=255)                                          cGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGcc                                                                                                            >  2:1102908/1‑123 (MQ=255)                                          cGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGcc                                                                                                            <  1:1102908/123‑1 (MQ=255)                                          cGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGa                                                                                           <  2:927061/140‑1 (MQ=255)                                          cGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGa                                                                                           <  1:195232/140‑1 (MQ=255)                                             tgctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAAc                                                                                                      <  2:309543/126‑1 (MQ=255)                                             tgctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAAc                                                                                                      >  1:309543/1‑126 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGtt                                                                                                 >  2:667290/1‑129 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGtt                                                                                                 <  1:667290/129‑1 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGAt                                                                                          <  1:1070341/136‑1 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGAt                                                                                          >  2:1070341/1‑136 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg                                                                                      >  2:1129441/1‑140 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg                                                                                      >  2:1202135/1‑140 (MQ=255)                                               ctTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAgg                                                                                      >  1:990409/1‑140 (MQ=255)                                                tttGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGt                                                                                     <  1:1202135/140‑1 (MQ=255)                                                tttGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGt                                                                                     <  1:916948/140‑1 (MQ=255)                                                    tttACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGat                                                                                 <  1:1006610/140‑1 (MQ=255)                                                    tttACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGat                                                                                 >  1:420027/1‑140 (MQ=255)                                                         cGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCg                                                                            >  2:1051544/1‑140 (MQ=255)                                                         cGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCg                                                                            >  2:628446/1‑140 (MQ=255)                                                         cGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCg                                                                            >  1:934197/1‑140 (MQ=255)                                                           gACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGcc                                                                          >  2:139859/1‑140 (MQ=255)                                                            aCCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCa                                                                         <  2:841889/140‑1 (MQ=255)                                                              cGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga                                                                       >  1:909702/1‑140 (MQ=255)                                                              cGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga                                                                       <  2:909702/140‑1 (MQ=255)                                                              cGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga                                                                       <  1:1201817/140‑1 (MQ=255)                                                                          tACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac                                                               <  2:11135/136‑1 (MQ=255)                                                                          tACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGaccac                                                               >  1:11135/1‑136 (MQ=255)                                                                          tACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGa                                                           >  1:122067/1‑140 (MQ=255)                                                                               atcatGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga                                                                       <  2:644950/123‑1 (MQ=255)                                                                               atcatGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAga                                                                       >  1:644950/1‑123 (MQ=255)                                                                               atcatGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa                                                      >  2:1013559/1‑140 (MQ=255)                                                                               atcatGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa                                                      >  2:93031/1‑140 (MQ=255)                                                                               atcatGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGa                                                      >  1:1032519/1‑140 (MQ=255)                                                                                         cGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGa                                            <  1:1013559/140‑1 (MQ=255)                                                                                         cGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGa                                            >  1:285809/1‑140 (MQ=255)                                                                                         cGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGa                                            <  1:65921/140‑1 (MQ=255)                                                                                         cGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGa                                            <  1:340893/140‑1 (MQ=255)                                                                                             gATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGa                                        <  1:570837/140‑1 (MQ=255)                                                                                                  cccccAGCAGAACCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAgg                                   >  1:758255/1‑140 (MQ=255)                                                                                                             aCCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAg                        <  2:970585/140‑1 (MQ=255)                                                                                                             aCCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAg                        <  2:122067/140‑1 (MQ=255)                                                                                                             aCCGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAg                        <  2:898274/140‑1 (MQ=255)                                                                                                               cGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGTCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAgcg                      <  2:229343/140‑1 (MQ=255)                                                                                                               cGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAgcg                      <  2:934197/140‑1 (MQ=255)                                                                                                               cGCATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAgcg                      <  1:1129441/140‑1 (MQ=255)                                                                                                                 cATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCTCCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGcc                    >  1:767079/1‑140 (MQ=255)                                                                                                                 cATTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGcc                    >  2:413480/1‑140 (MQ=255)                                                                                                                  aTTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAgcg                      >  1:56184/1‑137 (MQ=255)                                                                                                                  aTTCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAgcg                      <  2:56184/137‑1 (MQ=255)                                                                                                                   ttCGCAGTCCCCATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTc                  >  2:772581/1‑140 (MQ=255)                                                                                                                             ccATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATGCTCtgt        <  2:419669/140‑1 (MQ=255)                                                                                                                              cATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCtgtg       <  2:810256/140‑1 (MQ=255)                                                                                                                              cATGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCtgtg       <  1:105824/140‑1 (MQ=255)                                                                                                                               aTGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCt                   >  1:526133/1‑127 (MQ=255)                                                                                                                               aTGGTCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCt                   <  2:526133/127‑1 (MQ=255)                                                                                                                                   tCTACAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGt  <  2:285809/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CCATAGGTTTGAGTACTCAGCAGACCCACACCGAATGCGTACGCTGCTTTGTTTACCGGACCGCCCATGTCAGTACACATCATGCCACCGAGGATCGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGT  >  NC_000913/2260128‑2260397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑