Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,345,426	A→G	T188A (ACG→GCG)	nrdA →	ribonucleoside‑diphosphate reductase 1 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,345,426	0	A	G	100.0%	173.4 / NA	47	T188A (ACG→GCG)	nrdA	ribonucleoside‑diphosphate reductase 1 subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/23);  total (24/23)

CTTCTCTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACA  >  NC_000913/2345290‑2345561                                                                                                                                         |                                                                                                                                        cTTCTCTTATGCTGCCGTTAAGCAGCTGGAAGGCAAA‑ATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGcgcg                                                                                                                                     <  2:339975/140‑1 (MQ=255)  ttCTCTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGcgcg                                                                                                                                     <  1:526823/140‑1 (MQ=255)   tctcTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAgcgcgc                                                                                                                                    >  2:502022/1‑140 (MQ=255)   tctcTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAgcgcgc                                                                                                                                    <  2:338322/140‑1 (MQ=255)   tctcTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAgcgcgc                                                                                                                                    >  2:672963/1‑140 (MQ=255)    ctctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGcc                                                                                                                                   >  1:624816/1‑140 (MQ=255)    ctctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGcc                                                                                                                                   <  1:444710/140‑1 (MQ=255)         aTGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCaa                                                                                                                              <  2:36736/140‑1 (MQ=255)           gctgcCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAata                                                                                                                            >  2:557632/1‑140 (MQ=255)               ccattAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGcg                                                                                                                                       >  1:364863/4‑125 (MQ=255)               ccGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATAtgtg                                                                                                                        >  2:48030/1‑140 (MQ=255)               ccGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATAtgtg                                                                                                                        >  2:813032/1‑140 (MQ=255)               ccGTTAAACAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATAtgtg                                                                                                                        >  2:607673/1‑140 (MQ=255)                               aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTacgacg                                                                                                        <  2:624816/140‑1 (MQ=255)                               aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTacgacg                                                                                                        <  1:372035/140‑1 (MQ=255)                                                  aaCCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAAt                                                                                     >  1:940821/1‑140 (MQ=255)                                                  aaCCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAAt                                                                                     >  2:192321/1‑140 (MQ=255)                                                       cgTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGGACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGc                                                                                >  2:1093987/1‑140 (MQ=255)                                                           aCCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGcc                                                                            <  1:722102/140‑1 (MQ=255)                                                                      cTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCa                                                                 <  1:48030/140‑1 (MQ=255)                                                                         tGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGt                                                              <  1:770842/140‑1 (MQ=255)                                                                          gagaGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTc                                                             >  2:377955/1‑140 (MQ=255)                                                                              gcgcCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGgc                                                         >  2:862867/1‑140 (MQ=255)                                                                               cgcCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGgcg                                                        >  2:557747/1‑140 (MQ=255)                                                                               cgcCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGgcg                                                        >  2:768904/1‑140 (MQ=255)                                                                                 cccAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGt                                                              >  2:570142/1‑132 (MQ=255)                                                                                 cccAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGt                                                              <  1:570142/132‑1 (MQ=255)                                                                                           ttATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGa                                            <  1:813032/140‑1 (MQ=255)                                                                                                ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGgc                                                         >  2:257832/1‑122 (MQ=255)                                                                                                ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGgc                                                         <  1:257832/122‑1 (MQ=255)                                                                                                ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGa                                            >  2:363102/1‑135 (MQ=255)                                                                                                ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGa                                            <  1:363102/135‑1 (MQ=255)                                                                                                ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGt                                       >  2:299130/1‑140 (MQ=255)                                                                                                ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGt                                       >  2:735511/1‑140 (MQ=255)                                                                                                   tAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAg                                    <  1:735511/140‑1 (MQ=255)                                                                                                        gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAg                               >  2:101015/1‑140 (MQ=255)                                                                                                             gTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCt                          <  1:877644/140‑1 (MQ=255)                                                                                                             gTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCt                          >  2:877644/1‑140 (MQ=255)                                                                                                             gTGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCt                          <  2:940821/140‑1 (MQ=255)                                                                                                              tGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTg                         <  2:327520/140‑1 (MQ=255)                                                                                                              tGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTg                         <  1:607673/140‑1 (MQ=255)                                                                                                              tGCTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGCGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTg                         <  1:502022/140‑1 (MQ=255)                                                                                                                cTTGTTCTCGAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCg                       <  1:1106970/140‑1 (MQ=255)                                                                                                                         gAACTACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCg              <  1:833455/140‑1 (MQ=255)                                                                                                                             tACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACGTTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTg          >  2:845409/1‑140 (MQ=255)                                                                                                                             tACCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTg          <  1:1093987/140‑1 (MQ=255)                                                                                                                              aCCCGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGc         >  2:357702/1‑140 (MQ=255)                                                                                                                               cccGCGTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCg        >  2:944285/1‑140 (MQ=255)                                                                                                                                  gcgTGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTg     <  1:357702/140‑1 (MQ=255)                                                                                                                                     tGAAGCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACa  >  2:245257/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTTCTCTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACA  >  NC_000913/2345290‑2345561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑