Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	163,298	A→G	A398A (GCA→GCG)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	163,298	0	A	G	100.0%	149.3 / NA	40	A398A (GCA→GCG)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/17);  total (23/17)

CAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCT  >  NC_000913/163164‑163432                                                                                                                                       |                                                                                                                                       aaaaGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAAGGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGa                                                                                                                                   <  1:980639/139‑1 (MQ=255)     gCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGt                                                                                                                               >  1:1012545/1‑140 (MQ=255)                   ttGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTgc                                                                                                                 >  2:462489/1‑140 (MQ=255)                    tGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTgcg                                                                                                                >  2:81057/1‑140 (MQ=255)                     gCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTgcgc                                                                                                               <  1:1142767/140‑1 (MQ=255)                            aaaTTACTGCAAGGGGGATGCAGCGACCCGGCGAAGATGAGCGGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCa                                                                                                        <  1:894410/139‑1 (MQ=255)                                       aTGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAg                                                                                             >  1:895534/1‑140 (MQ=255)                                        tGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgc                                                                                            >  1:969083/1‑140 (MQ=255)                                        tGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgc                                                                                            >  1:656792/1‑140 (MQ=255)                                         ggggCTGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcg                                                                                           <  2:764543/140‑1 (MQ=255)                                         ggggATGCAGCGATCCGGCGCAGATGAGCTGGTTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcg                                                                                           <  1:325462/140‑1 (MQ=255)                                         ggggATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcg                                                                                           <  2:969083/140‑1 (MQ=255)                                         ggggATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAgcg                                                                                           <  1:81057/140‑1 (MQ=255)                                            gATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATgg                                                                                                <  2:344057/132‑1 (MQ=255)                                            gATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATgg                                                                                                >  1:344057/1‑132 (MQ=255)                                                    gATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGa                                                                                >  1:1011708/1‑140 (MQ=255)                                                                aTGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGc                                                                    >  1:436600/1‑140 (MQ=255)                                                                  gAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCgg                                                                  <  2:348804/140‑1 (MQ=255)                                                                  gAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCgg                                                                  <  2:1011708/140‑1 (MQ=255)                                                                     ctggctggAACAACCGCCAGTAGTGAATCTACTGGTCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGGTTAGCGGCaa                                                               >  2:365529/1‑140 (MQ=255)                                                                          tggATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTg                                                          >  1:178590/1‑140 (MQ=255)                                                                                  ccGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAgcgc                                                  >  1:43612/1‑140 (MQ=255)                                                                                   cGCCAGTAGTGAATCTACTGGTCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGcgcg                                                 <  1:365529/140‑1 (MQ=255)                                                                                      cAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCgaag                                              >  2:787841/1‑140 (MQ=255)                                                                                         tagtGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAac                                           <  2:932276/140‑1 (MQ=255)                                                                                         tagtGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAac                                           <  2:43612/140‑1 (MQ=255)                                                                                              aaTCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAacgacga                                      >  1:404307/1‑140 (MQ=255)                                                                                                tCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAacgacgacg                                    <  2:1072510/140‑1 (MQ=255)                                                                                                   aCTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAg                                 >  1:1133781/1‑140 (MQ=255)                                                                                                   aCTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAg                                 >  2:640608/1‑140 (MQ=255)                                                                                                   aCTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAg                                 >  1:1024585/1‑140 (MQ=255)                                                                                                           cgAAACGTCTGTTACAAATGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTAcc                         >  1:1024570/1‑140 (MQ=255)                                                                                                                            aTGCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTgccgc        <  2:1024570/140‑1 (MQ=255)                                                                                                                              gCTGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCa      <  1:787841/140‑1 (MQ=255)                                                                                                                                tGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCAtt    >  2:149056/1‑140 (MQ=255)                                                                                                                                tGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCAtt    >  2:752827/1‑140 (MQ=255)                                                                                                                                tGGGGGCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGGAACGATCCGCGTTTAGCGGCAAGGCTGGGTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCAtt    >  2:23140/1‑140 (MQ=255)                                                                                                                                 gggggCGCTGGAGGGTGAACGGCTTAGTGCGCAAGTGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTgccgc        >  2:1020699/1‑135 (MQ=255)                                                                                                                                 gggggCGCTGGAGGGTGAACGGCTTAGTGCGCAAGTGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTgccgc        <  1:1020699/135‑1 (MQ=255)                                                                                                                                 gggggCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATtc   >  1:916585/1‑140 (MQ=255)                                                                                                                                  ggggCGCTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATtct  >  1:716377/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCT  >  NC_000913/163164‑163432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑