Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,314	G→A	G149G (GGC→GGT)	dapA ←	4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,314	0	G	A	100.0%	218.3 / NA	62	G149G (GGC→GGT)	dapA	4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/35);  total (27/35)

AGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTGTAG  >  NC_000913/2599181‑2599443                                                                                                                                      |                                                                                                                                  aGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCAcc                                                                                                                             >  1:781147/1‑140 (MQ=255)      aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTc                                                                                                                        >  2:621376/1‑140 (MQ=255)       gcgcgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGGTTcc                                                                                                                       >  2:1106982/1‑140 (MQ=255)        cgcgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCg                                                                                                                      >  2:789814/1‑140 (MQ=255)            cTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAg                                                                                                                  >  1:461511/1‑140 (MQ=255)             tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGc                                                                                                                 <  1:754331/140‑1 (MQ=255)             tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGc                                                                                                                 >  2:784326/1‑140 (MQ=255)               gCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAg                                                                                                               >  1:1016109/1‑140 (MQ=255)                    atcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGc                                                                                                          <  1:987175/140‑1 (MQ=255)                     tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCa                                                                                                         <  2:474988/140‑1 (MQ=255)                       gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                                       <  2:649568/140‑1 (MQ=255)                       gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                                       >  1:474594/1‑140 (MQ=255)                       gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                                       <  2:781147/140‑1 (MQ=255)                       gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                                       <  1:621376/140‑1 (MQ=255)                            tCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTa                                                                                                  <  1:285145/140‑1 (MQ=255)                              agcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACg                                                                                                <  2:474594/140‑1 (MQ=255)                              agcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACg                                                                                                <  1:1103924/140‑1 (MQ=255)                               gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACgg                                                                                               <  1:784326/140‑1 (MQ=255)                                cagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACggg                                                                                              >  1:51881/1‑140 (MQ=255)                                 agAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                             <  2:679947/140‑1 (MQ=255)                                 agAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                             <  1:789814/140‑1 (MQ=255)                                   aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                                                                                           <  1:392049/140‑1 (MQ=255)                                   aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                                                                                           <  1:770553/140‑1 (MQ=255)                                      aaaaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCa                                                                                                         <  1:524473/123‑1 (MQ=255)                                      aaaaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCa                                                                                                         >  2:524473/1‑123 (MQ=255)                                        aaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcaca                                                                                      <  1:540788/140‑1 (MQ=255)                                        aaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcaca                                                                                      >  2:498372/1‑140 (MQ=255)                                             tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtata                                                                                 >  1:695154/1‑140 (MQ=255)                                                        ctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCg                                                                      <  2:461511/140‑1 (MQ=255)                                                         tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg                                                                     <  2:64669/140‑1 (MQ=255)                                                           tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa                                                                   <  2:51881/140‑1 (MQ=255)                                                           tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa                                                                   <  1:1106982/140‑1 (MQ=255)                                                           tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa                                                                   <  2:184237/140‑1 (MQ=255)                                                             tGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg                                                                 <  2:925563/140‑1 (MQ=255)                                                             tGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg                                                                 <  2:1120734/140‑1 (MQ=255)                                                                tCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCa                                                              <  1:498372/140‑1 (MQ=255)                                                                       tACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCt                                                       <  2:903519/140‑1 (MQ=255)                                                                         cacGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCa                                                     >  2:25802/1‑140 (MQ=255)                                                                              gTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGAt                                                <  1:126366/140‑1 (MQ=255)                                                                                        cTGTTGCCTCTTTGATTTCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGaaa                                      >  2:441331/1‑140 (MQ=255)                                                                                        cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCg                                                  <  1:1028672/128‑1 (MQ=255)                                                                                        cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCg                                                  >  2:1028672/1‑128 (MQ=255)                                                                                        cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGaaa                                      >  1:779843/1‑140 (MQ=255)                                                                                        cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGaaa                                      >  1:1123933/1‑140 (MQ=255)                                                                                          gTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATg                                    <  1:835678/140‑1 (MQ=255)                                                                                          gTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATg                                    >  2:872801/1‑140 (MQ=255)                                                                                              ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCg                                                  <  2:696026/122‑1 (MQ=255)                                                                                              ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCg                                                  >  1:696026/1‑122 (MQ=255)                                                                                              ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGa                                <  1:1054222/140‑1 (MQ=255)                                                                                                tctTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATa                              >  2:364327/1‑140 (MQ=255)                                                                                                 ctTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATAc                             >  2:393239/1‑140 (MQ=255)                                                                                                 ctTTGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATAc                             >  2:193693/1‑140 (MQ=255)                                                                                                  tttGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGc                                   <  2:557627/133‑1 (MQ=255)                                                                                                  tttGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGc                                   >  1:557627/1‑133 (MQ=255)                                                                                                    tGATTCCGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACaaa                          <  2:85627/140‑1 (MQ=255)                                                                                                         ccGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACcttc                     >  1:223333/1‑140 (MQ=255)                                                                                                          cGATAATATTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACcttct                    >  2:845672/1‑140 (MQ=255)                                                                                                               atatTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCga               <  1:25802/140‑1 (MQ=255)                                                                                                                tatTTTTTACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgac              >  2:36051/1‑140 (MQ=255)                                                                                                                   tttttACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAGTGCTGATACAAACCTTCTTGCgacgga           <  1:265562/140‑1 (MQ=255)                                                                                                                   tttttACTTTCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga           <  2:779843/140‑1 (MQ=255)                                                                                                                            tCGCCAGACGACCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTgtag  >  2:701759/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                  AGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTGTAG  >  NC_000913/2599181‑2599443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑