Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,759,005	C→T	F7F (TTC→TTT)	yfjI →	CP4‑57 prophage; protein YfjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,759,005	0	C	T	100.0%	250.9 / NA	69	F7F (TTC→TTT)	yfjI	CP4‑57 prophage; protein YfjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/25);  total (44/25)

TTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGATG  >  NC_000913/2758871‑2759138                                                                                                                                       |                                                                                                                                      ttCATCCTCAATTGATGTAATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGt                                                                                                                                  <  2:986914/140‑1 (MQ=255)     tACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGAt                                                                                                                              >  1:530825/1‑140 (MQ=255)      aCTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATg                                                                                                                             >  2:1094068/1‑140 (MQ=255)       cTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGc                                                                                                                            >  1:87169/1‑140 (MQ=255)          aaTTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCAtt                                                                                                                         >  1:1100515/1‑140 (MQ=255)            ttGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTc                                                                                                                       >  2:1046896/1‑140 (MQ=255)             tGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTcc                                                                                                                      <  2:318608/140‑1 (MQ=255)                  tCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTaaaa                                                                                                                 <  1:1169545/140‑1 (MQ=255)                  tCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTaaaa                                                                                                                 >  2:277630/1‑140 (MQ=255)                    aTCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAAtt                                                                                                               >  1:416468/1‑140 (MQ=255)                           tgtAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGa                                                                                                        >  1:910529/1‑140 (MQ=255)                            gtAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGaa                                                                                                       <  2:156459/140‑1 (MQ=255)                               aaCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGc                                                                                                    <  1:468932/140‑1 (MQ=255)                                   tCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAtt                                                                                                >  2:472379/1‑140 (MQ=255)                                   tCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAtt                                                                                                >  2:161976/1‑140 (MQ=255)                                     cTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTa                                                                                              >  1:435918/1‑140 (MQ=255)                                     cTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTa                                                                                              >  2:244568/1‑140 (MQ=255)                                     cTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTa                                                                                              >  2:474702/1‑140 (MQ=255)                                     cTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTa                                                                                              <  2:910529/140‑1 (MQ=255)                                     cTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTa                                                                                              >  2:99381/1‑140 (MQ=255)                                       gCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATg                                                                                            <  1:1046896/140‑1 (MQ=255)                                       gCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATg                                                                                            <  2:34050/140‑1 (MQ=255)                                       gCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATg                                                                                            >  2:377541/1‑140 (MQ=255)                                       gCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATg                                                                                            >  1:68995/1‑140 (MQ=255)                                             tCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTgg                                                                                      <  1:755963/140‑1 (MQ=255)                                              ccGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGa                                                                                     <  2:530825/140‑1 (MQ=255)                                              ccGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGa                                                                                     <  2:1015334/140‑1 (MQ=255)                                                    ttAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCa                                                                               <  2:68995/140‑1 (MQ=255)                                                           cGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAg                                                                        <  2:174883/140‑1 (MQ=255)                                                              aaTAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGcc                                                                     >  1:1195231/1‑140 (MQ=255)                                                              aaTAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGcc                                                                     >  2:590184/1‑140 (MQ=255)                                                              aaTAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGcc                                                                     >  1:294286/1‑140 (MQ=255)                                                                tAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGcccc                                                                   >  1:897371/1‑140 (MQ=255)                                                                      tatTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAgg                                                             <  1:590184/140‑1 (MQ=255)                                                                            cTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGAt                                                       >  2:1038672/1‑140 (MQ=255)                                                                            cTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGAt                                                       >  2:1087055/1‑140 (MQ=255)                                                                             tAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGAtt                                                      >  2:721255/1‑140 (MQ=255)                                                                             tAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGAtt                                                      >  2:40054/1‑140 (MQ=255)                                                                               aaCAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATtgc                                                    <  1:161976/140‑1 (MQ=255)                                                                               aaCAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTACCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATtgc                                                    <  2:1100515/140‑1 (MQ=255)                                                                                 cAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATtgctg                                                  <  2:644264/140‑1 (MQ=255)                                                                                      tCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCt                                             <  2:87169/140‑1 (MQ=255)                                                                                         taatttaattAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGct                                          >  2:507660/1‑140 (MQ=255)                                                                                                       aaGAGGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTtc                            <  1:472379/140‑1 (MQ=255)                                                                                                         gagGCATTGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTtctc                          >  2:297539/1‑140 (MQ=255)                                                                                                               ttGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCt                    <  1:790406/140‑1 (MQ=255)                                                                                                                tGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTtctc                          <  2:211306/133‑1 (MQ=255)                                                                                                                tGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTtctc                          >  1:211306/1‑133 (MQ=255)                                                                                                                tGCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTg                   >  2:169254/1‑140 (MQ=255)                                                                                                                 gCATGTTCAATGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGt                  <  1:721255/140‑1 (MQ=255)                                                                                                                          aTGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCgg         >  2:623101/1‑140 (MQ=255)                                                                                                                          aTGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCgg         >  2:122594/1‑140 (MQ=255)                                                                                                                          aTGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCgg         >  2:826745/1‑140 (MQ=255)                                                                                                                           tGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGa        <  1:1038672/140‑1 (MQ=255)                                                                                                                           tGGTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCACTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGa        >  2:1076217/1‑140 (MQ=255)                                                                                                                            ggTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGAt       >  2:793110/1‑140 (MQ=255)                                                                                                                            ggTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGAt       >  1:973078/1‑140 (MQ=255)                                                                                                                            ggTCGTCCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGAt       <  1:1142184/140‑1 (MQ=255)                                                                                                                              tcgtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTg     >  2:339847/1‑140 (MQ=255)                                                                                                                              tcgtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTg     >  1:547987/1‑140 (MQ=255)                                                                                                                                gtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGAt   >  1:31074/1‑140 (MQ=255)                                                                                                                                gtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGAt   >  2:620304/1‑140 (MQ=255)                                                                                                                                gtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGAt   >  1:738228/1‑140 (MQ=255)                                                                                                                                gtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGAt   >  2:1014142/1‑140 (MQ=255)                                                                                                                                gtcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGAt   >  1:953477/1‑140 (MQ=255)                                                                                                                                 tcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGa        <  2:759141/134‑1 (MQ=255)                                                                                                                                 tcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGa        >  1:759141/1‑134 (MQ=255)                                                                                                                                 tcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGATg  >  1:467319/1‑140 (MQ=255)                                                                                                                                 tcCTTTTCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGATg  >  1:791967/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      TTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGGGTAATTTCTCTTGCCTGTCAGAACCGGATTGATG  >  NC_000913/2758871‑2759138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑