Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,779,929	T→C	N933S (AAC→AGC)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,779,929	0	T	C	100.0%	190.4 / NA	54	N933S (AAC→AGC)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/32);  total (22/32)

TGCGTAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTA  >  NC_000913/2779796‑2780056                                                                                                                                      |                                                                                                                                tGCGTAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCa                                                                                                                           <  1:1174026/140‑1 (MQ=255) tGCGTAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCa                                                                                                                           <  1:732791/140‑1 (MQ=255) tGCGTAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCa                                                                                                                           <  2:223244/140‑1 (MQ=255) cgCGTAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCa                                                                                                                           <  2:920091/139‑1 (MQ=255)     tAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGc                                                                                                                       >  1:348494/1‑140 (MQ=255)        aCGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGcccc                                                                                                                    >  2:623635/1‑140 (MQ=255)         cGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCa                                                                                                                   <  1:219091/140‑1 (MQ=255)         cGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCa                                                                                                                   <  1:605494/140‑1 (MQ=255)          gATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCAc                                                                                                                  >  1:516296/1‑140 (MQ=255)              gCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCtt                                                                                                              <  1:665757/140‑1 (MQ=255)                 gTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGcc                                                                                                           <  1:623635/140‑1 (MQ=255)                      gCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCg                                                                                                      >  1:187302/1‑140 (MQ=255)                      gCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTCTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCg                                                                                                      >  1:1093083/1‑140 (MQ=255)                       ccAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGTCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGt                                                                                                     >  2:518828/1‑140 (MQ=255)                       ccAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGt                                                                                                     >  1:575426/1‑140 (MQ=255)                         aGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTgg                                                                                                   >  1:19408/1‑140 (MQ=255)                              ccTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTcg                                                                                              >  2:1002500/1‑140 (MQ=255)                              ccTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTcg                                                                                              <  2:19408/140‑1 (MQ=255)                                tGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTcgcg                                                                                            <  1:952160/140‑1 (MQ=255)                                tGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTcgcg                                                                                            <  1:912284/140‑1 (MQ=255)                                 ggAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGa                                                                                           <  2:84551/140‑1 (MQ=255)                                    gaTCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCt                                                                                        <  2:575426/140‑1 (MQ=255)                                       cttctGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGCATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGACCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGAGGAGTCGCGAGCTTCg                                                                                     >  1:213530/1‑140 (MQ=255)                                          ctGCCGGGGCCAGAGCCTGAAGCTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTATGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGtc                                                                                  <  2:887027/140‑2 (MQ=255)                                          ctGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTa                                                                                  <  2:835780/140‑1 (MQ=255)                                          ctGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTa                                                                                  <  2:187302/140‑1 (MQ=255)                                          ctGCCCGGGCCAGAGCCTGAAACTCATCTTCTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTa                                                                                  <  2:1093083/140‑1 (MQ=255)                                           tGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTAc                                                                                 <  2:643503/140‑1 (MQ=255)                                           tGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACACAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTAc                                                                                 <  2:84371/140‑1 (MQ=255)                                            gCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTAcc                                                                                >  2:844348/1‑140 (MQ=255)                                                gggCCAGATCCTAATACTCCGCTTTTGTCCCCGCACGGGTATATTCCCCGCCCGAGTTAACCTTTGTGGCGGAGTACTGTCCCTGGTTTTGCATTGCCCCACCCTTGCCCACCGGGGGGTTGCGAGCGTCGGTACCTGCt                                                                            <  2:813097/140‑1 (MQ=255)                                                gggCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCt                                                                            <  1:844348/140‑1 (MQ=255)                                                   ccAGAGCCTGAAACTCATCTTTTTACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCGTTGCCCCACCCTAGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAAt                                                                         <  1:1086319/140‑1 (MQ=255)                                                     agagCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATAc                                                                       <  2:435380/140‑1 (MQ=255)                                                            gAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATaaa                                                                >  2:776167/1‑140 (MQ=255)                                                            gAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGACCCACCCTTGCCAACCGCGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATaaa                                                                >  1:1021622/1‑140 (MQ=255)                                                                          gACCGCCCAAGGGTATATTGTCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTc                                                  <  1:518828/140‑1 (MQ=255)                                                                                         tatTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACTGCCATTTTCCAGCAACATATTGGt                                   >  2:875942/1‑140 (MQ=255)                                                                                          atTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGc                                              <  1:558877/128‑1 (MQ=255)                                                                                          atTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGc                                              >  2:558877/1‑128 (MQ=255)                                                                                              cccGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTa                              >  1:242191/1‑140 (MQ=255)                                                                                              cccGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATA‑TGGTCGCTaa                             >  2:1163081/1‑140 (MQ=255)                                                                                               ccGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTaa                             >  2:503205/1‑140 (MQ=255)                                                                                                cGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTaaa                            >  2:765241/1‑140 (MQ=255)                                                                                                cGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTaaa                            <  1:765241/140‑1 (MQ=255)                                                                                                cGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTaaa                            >  2:227209/1‑140 (MQ=255)                                                                                                cGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTaaa                            >  2:73397/1‑140 (MQ=255)                                                                                                          aaCCTTTGTGGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATA‑TGGTCGCTAAATTGCCGGAAAt                 <  1:1163081/140‑1 (MQ=255)                                                                                                              tttGGGGGGGAGTCCTGACCCAGCCTTTGCATTGCCCCCCCCTTCCCACCCGTGGAGTCGCGAGCTTCGGTGCCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGa              <  2:794503/140‑1 (MQ=255)                                                                                                                tgtgGCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGaaa            >  1:698233/1‑140 (MQ=255)                                                                                                                   ggCGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAg         <  1:776167/140‑1 (MQ=255)                                                                                                                     cGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACTGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTa       <  1:875942/140‑1 (MQ=255)                                                                                                                     cGGAGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTa       <  1:503205/140‑1 (MQ=255)                                                                                                                     cGGAGTCCTGACCCAGGCTTTGCATTGACCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTa       <  2:1021622/140‑1 (MQ=255)                                                                                                                        aGTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCg    <  2:698233/140‑1 (MQ=255)                                                                                                                         gTCCTGACCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTAGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCAGAAATGGAAAAAGTACCGt   >  1:109072/1‑140 (MQ=255)                                                                                                                          tCCTGCCCCAGGCTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTa  <  2:348494/140‑1 (MQ=255)                                                                                                                          tCCTGACCCAGGCTTTGCATTGCCCCCCCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTa  <  1:227209/140‑1 (MQ=255)                                                                                                                          tCCTGACCCAGGCTTTGCATTGCCCCCCCCTTGCCAACCGTGGAGTAGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCAGAAATGGAAAAAGTACCGTa  <  2:109072/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TGCGTAGACGATTGCCGTACCGCCAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTA  >  NC_000913/2779796‑2780056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑