| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,835,958 | G→A | P490S (CCG→TCG) | hypF ← | carbamoyltransferase HypF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,835,958 | 0 | G | A | 98.0% | 161.8 / NA | 49 | P490S (CCG→TCG) | hypF | carbamoyltransferase HypF |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (25/23); minor base C (0/1); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACG > NC_000913/2835828‑2836082 | gCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATg < 1:840426/140‑1 (MQ=255) aGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGct > 2:1050072/1‑140 (MQ=255) aGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGcc < 2:553977/140‑1 (MQ=255) cAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACAcg > 1:332633/1‑140 (MQ=255) gTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACAcgccg < 1:271649/140‑1 (MQ=255) cTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTg > 1:152372/1‑140 (MQ=255) tCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGc < 1:1050072/140‑1 (MQ=255) tCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGc < 1:832672/140‑1 (MQ=255) acaTTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCAt < 1:945990/140‑1 (MQ=255) ttCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATggg < 1:628222/140‑1 (MQ=255) tCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCAt > 2:120763/1‑136 (MQ=255) tCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCAt < 1:120763/136‑1 (MQ=255) cgcgATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCa > 1:517875/1‑140 (MQ=255) gATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatga < 2:1143416/140‑1 (MQ=255) cAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCa > 2:231792/1‑124 (MQ=255) cAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCa < 1:231792/124‑1 (MQ=255) cAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCg > 2:536711/1‑140 (MQ=255) ggCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGtt < 2:152372/140‑1 (MQ=255) ccccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAg > 1:129805/1‑140 (MQ=255) ccccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAg > 2:712533/1‑140 (MQ=255) cccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGa > 1:104902/1‑140 (MQ=255) gcCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCAc > 1:106817/1‑140 (MQ=255) cGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCAcgc > 2:161162/1‑140 (MQ=255) tcCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCa < 2:832101/140‑1 (MQ=255) tcCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCa < 2:517875/140‑1 (MQ=255) cccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCAc < 2:1103023/140‑1 (MQ=255) taccgataccgCCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGCCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActggctg < 2:1201557/140‑1 (MQ=255) accgataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActggctgg > 2:1122726/1‑140 (MQ=255) ataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActggctgg < 2:196777/136‑1 (MQ=255) ataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActggctgg > 1:196777/1‑136 (MQ=255) ataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAc > 2:868161/1‑140 (MQ=255) ataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAc > 2:1012029/1‑140 (MQ=255) gTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCa < 2:476704/123‑1 (MQ=255) gTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCa > 1:476704/1‑123 (MQ=255) gTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGc > 1:157602/1‑140 (MQ=255) tCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActggctg > 1:74174/1‑129 (MQ=255) tCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActggctg < 2:74174/129‑1 (MQ=255) tCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActg < 2:1157694/125‑1 (MQ=255) tCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCActg > 1:1157694/1‑125 (MQ=255) tCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGcc > 1:974385/1‑140 (MQ=255) gAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGTATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCg > 2:560023/1‑140 (MQ=255) gAGCGTCAAAGCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCg > 1:199835/1‑140 (MQ=255) gCAATGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGTATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTc < 1:560023/140‑1 (MQ=255) aaTGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCAt < 2:106817/140‑1 (MQ=255) aaTGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCAt < 2:129805/140‑1 (MQ=255) tGACATCACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGc < 1:536711/140‑1 (MQ=255) cACCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACg > 2:639310/1‑140 (MQ=255) aCCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGt > 1:414796/1‑128 (MQ=255) aCCGCCATCCAGCGACCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGt < 2:414796/128‑1 (MQ=255) | GCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACG > NC_000913/2835828‑2836082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |