Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,839,743	G→A	A74T (GCC→ACC)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,839,743	0	G	A	100.0%	281.5 / NA	80	A74T (GCC→ACC)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/40);  total (40/40)

TATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAGCCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGAT  >  NC_000913/2839607‑2839876                                                                                                                                         |                                                                                                                                      tATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCt                                                                                                                                    >  1:444399/1‑140 (MQ=255)       gcgGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAgg                                                                                                                              >  2:768252/1‑140 (MQ=255)         ggTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGaa                                                                                                                            >  2:402460/1‑140 (MQ=255)         ggTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGaa                                                                                                                            >  1:58394/1‑140 (MQ=255)         ggTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGaa                                                                                                                            >  1:386080/1‑140 (MQ=255)           ttGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAgt                                                                                                                          >  2:1124295/1‑140 (MQ=255)           ctGCGCTTTGTTATCAAAGATGATGCACTTATTGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAgt                                                                                                                          >  1:1176340/2‑140 (MQ=255)              ggcTTTGTTATCAAAGATGATGCACTTATCGACAGCGCGACGTTAAAAACCATCCCCGACGTGCTCGGCGTGGTGCGTAGTGACATCCAGTGTCAGGTGATTATCGGCAATACCGTTTGACAAACCTTTCAGGAAgtcgt                                                                                                                       <  2:1166765/139‑1 (MQ=255)                     ttATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCctg                                                                                                                <  1:1056309/140‑1 (MQ=255)                     ttATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCctg                                                                                                                <  1:768252/140‑1 (MQ=255)                          aaaGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGcc                                                                                                           <  1:870999/140‑1 (MQ=255)                             gatgatGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCggg                                                                                                        <  2:243441/140‑1 (MQ=255)                             gatgatGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCggg                                                                                                        >  1:760657/1‑140 (MQ=255)                              atgatgCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGga                                                                                                       <  2:386080/140‑1 (MQ=255)                                  tgCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGata                                                                                                   >  2:548053/1‑140 (MQ=255)                                  tgCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGata                                                                                                   >  2:545365/1‑140 (MQ=255)                                  tgCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGata                                                                                                   >  1:367915/1‑140 (MQ=255)                                  tgCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGata                                                                                                   >  2:512809/1‑140 (MQ=255)                                  tgCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGata                                                                                                   <  2:367915/140‑1 (MQ=255)                                   gCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGatat                                                                                                  <  2:858463/140‑1 (MQ=255)                                   gCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGatat                                                                                                  <  1:723512/140‑1 (MQ=255)                                       ttATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAg                                                                                              <  1:1067429/140‑1 (MQ=255)                                        tATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGcc                                                                                                           <  1:203380/126‑1 (MQ=255)                                        tATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGcc                                                                                                           >  2:203380/1‑126 (MQ=255)                                        tATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGatat                                                                                                  >  1:170133/1‑135 (MQ=255)                                        tATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGatat                                                                                                  <  2:170133/135‑1 (MQ=255)                                        tATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGc                                                                                             >  2:51923/1‑140 (MQ=255)                                         aTCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAg                                                                                              <  2:819777/138‑1 (MQ=255)                                         aTCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAg                                                                                              >  1:819777/1‑138 (MQ=255)                                         aTCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGcc                                                                                            >  1:1199142/1‑140 (MQ=255)                                             aCAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGca                                                                                        <  1:512809/140‑1 (MQ=255)                                             aCAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGca                                                                                        <  2:616946/140‑1 (MQ=255)                                              cAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATGCCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGcac                                                                                       <  1:292427/140‑1 (MQ=255)                                               aGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGcaca                                                                                      >  1:581538/1‑140 (MQ=255)                                                 cccGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGc                                                                                    >  2:40963/1‑140 (MQ=255)                                                  ccGACGTTAAAATCCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGcc                                                                                   <  1:402460/140‑1 (MQ=255)                                                   cGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGccc                                                                                  <  1:51923/140‑1 (MQ=255)                                                   cGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGccc                                                                                  >  1:301641/1‑140 (MQ=255)                                                        ttAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTggg                                                                             <  1:1124295/140‑1 (MQ=255)                                                        ttAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTggg                                                                             <  1:959310/140‑1 (MQ=255)                                                         tAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGt                                                                            <  2:301641/140‑1 (MQ=255)                                                         tAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGt                                                                            <  2:1189797/140‑1 (MQ=255)                                                                cATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCa                                                                     <  1:814451/140‑1 (MQ=255)                                                                cATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCa                                                                     >  1:1157491/1‑140 (MQ=255)                                                                 aTCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCaa                                                                    >  1:371671/1‑140 (MQ=255)                                                                  tCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCaaa                                                                   >  2:794142/1‑140 (MQ=255)                                                                             cTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACg                                                        <  1:83210/140‑1 (MQ=255)                                                                             cTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACg                                                        >  1:141337/1‑140 (MQ=255)                                                                                ggCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCg                                                     <  2:141337/140‑1 (MQ=255)                                                                                  cGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCa                                                   >  1:998337/1‑140 (MQ=255)                                                                                   gTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCAt                                                  <  2:1176340/140‑1 (MQ=255)                                                                                      gTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTgg                                               >  2:458248/1‑140 (MQ=255)                                                                                       tACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCAt                                                  <  2:639138/136‑1 (MQ=255)                                                                                       tACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCAt                                                  >  1:639138/1‑136 (MQ=255)                                                                                          gTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCg                                           <  1:1056963/140‑1 (MQ=255)                                                                                          gTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCg                                           <  2:444399/140‑1 (MQ=255)                                                                                            aGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCggg                                         <  2:581538/140‑1 (MQ=255)                                                                                               gACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGAt                                      <  2:566909/140‑1 (MQ=255)                                                                                                aCAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATc                                     >  2:858820/1‑140 (MQ=255)                                                                                                aCAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATc                                     >  1:104678/1‑140 (MQ=255)                                                                                                aCAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATc                                     >  2:239498/1‑140 (MQ=255)                                                                                                 cAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCAt                                                  >  1:866932/1‑126 (MQ=255)                                                                                                 cAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCAt                                                  <  2:866932/126‑1 (MQ=255)                                                                                                 cAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATcc                                    >  1:656878/1‑140 (MQ=255)                                                                                                 cAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATcc                                    >  1:18072/1‑140 (MQ=255)                                                                                                   aCCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTc                                  >  1:339614/1‑140 (MQ=255)                                                                                                   aCCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTc                                  >  2:182694/1‑140 (MQ=255)                                                                                                     cAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGa                                >  2:60853/1‑140 (MQ=255)                                                                                                      aGTGTCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGAt                               >  1:695596/1‑140 (MQ=255)                                                                                                         gtCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATgcg                            <  1:548053/140‑1 (MQ=255)                                                                                                         gtCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATgcg                            <  2:1199142/140‑1 (MQ=255)                                                                                                          tCAGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATgcgc                           <  1:40963/140‑1 (MQ=255)                                                                                                            aGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTg                         >  1:301907/1‑140 (MQ=255)                                                                                                            aGGTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTg                         >  1:1079273/1‑140 (MQ=255)                                                                                                              gTGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGAt                       <  2:308922/140‑1 (MQ=255)                                                                                                               tGATTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATc                      <  2:339614/140‑1 (MQ=255)                                                                                                                 aTTATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCgg                    <  1:182694/140‑1 (MQ=255)                                                                                                                  ttATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGc                   >  1:826088/1‑140 (MQ=255)                                                                                                                   tATCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCa                  <  1:794142/140‑1 (MQ=255)                                                                                                                    aTCGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCAc                 <  2:998337/140‑1 (MQ=255)                                                                                                                      cGGCAATACCGTTTCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCa               <  1:858820/140‑1 (MQ=255)                                                                                                                                   tCACAAACCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGAt  >  1:298570/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATCGGCAATACCGTTTCACAAGCCTTTCAGGAAGTCGTCAGCCTGCTGCCGGGAGATATGCAGCCCGCACAGCCCGTGGGTAAACCCAAACTCACGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGAT  >  NC_000913/2839607‑2839876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑