Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,901,288	G→A	V233I (GTC→ATC)	yqcE →	putative transport protein YqcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,901,288	0	G	A	100.0%	338.8 / NA	94	V233I (GTC→ATC)	yqcE	putative transport protein YqcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/49);  total (45/49)

TGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGCCCACTTGGCGG  >  NC_000913/2901151‑2901424                                                                                                                                          |                                                                                                                                         tgtgCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATc                                                                                                                                        >  1:267988/1‑140 (MQ=255)   tgCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCtt                                                                                                                                      >  2:778586/1‑140 (MQ=255)    gCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCTCCTGGTATTGCAGCATGGTGATTTTTGGCATCTTc                                                                                                                                     <  2:85928/140‑1 (MQ=255)    gCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTc                                                                                                                                     <  1:834590/140‑1 (MQ=255)     cTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCa                                                                                                                                    <  1:668610/140‑1 (MQ=255)     cTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCa                                                                                                                                    <  2:686541/140‑1 (MQ=255)        gTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCa                                                                                                                                 >  1:663762/1‑140 (MQ=255)         ttttttGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCAt                                                                                                                                <  1:132359/140‑1 (MQ=255)         ttttttGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCAt                                                                                                                                <  2:663762/140‑1 (MQ=255)           ttttGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCt                                                                                                                              <  1:778586/140‑1 (MQ=255)             ttGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTAc                                                                                                                            >  2:1112634/1‑140 (MQ=255)              tGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACg                                                                                                                           <  1:674943/140‑1 (MQ=255)              tGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACg                                                                                                                           <  1:978246/140‑1 (MQ=255)                          caacaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGtt                                                                                                               <  2:562557/140‑1 (MQ=255)                          caacaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGtt                                                                                                               <  2:401224/140‑1 (MQ=255)                          caacaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGtt                                                                                                               <  2:311667/140‑1 (MQ=255)                             caacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACt                                                                                                            >  1:287274/1‑140 (MQ=255)                             caacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACt                                                                                                            >  2:405110/1‑140 (MQ=255)                              aacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTc                                                                                                           >  2:358259/1‑140 (MQ=255)                              aacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTc                                                                                                           <  2:812453/140‑1 (MQ=255)                                  tGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTccacc                                                                                                       <  1:1145657/140‑1 (MQ=255)                                  tGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTccacc                                                                                                       <  1:405110/140‑1 (MQ=255)                                  tGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTccacc                                                                                                       >  1:696112/1‑140 (MQ=255)                                    cgcAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCaa                                                                                                     >  1:693514/1‑140 (MQ=255)                                    cgcAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCaa                                                                                                     <  1:494125/140‑1 (MQ=255)                                        gTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTAt                                                                                                 <  1:680866/140‑1 (MQ=255)                                         tGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATc                                                                                                >  1:56386/1‑140 (MQ=255)                                            cAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGa                                                                                             <  1:849522/140‑1 (MQ=255)                                            cAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGa                                                                                             <  1:358259/140‑1 (MQ=255)                                              aTAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGAcc                                                                                           <  1:645422/140‑1 (MQ=255)                                               tAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCg                                                                                          <  2:709554/140‑1 (MQ=255)                                                 aCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGaa                                                                                        <  2:190793/140‑1 (MQ=255)                                                   gaagaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAAt                                                                                      >  1:909789/1‑140 (MQ=255)                                                   gaagaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAAt                                                                                      >  1:395142/1‑140 (MQ=255)                                                   gaagaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAAt                                                                                      >  2:313131/1‑140 (MQ=255)                                                     agaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGt                                                                                    >  1:497086/1‑140 (MQ=255)                                                         aaaCAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATgg                                                                                >  2:892255/1‑140 (MQ=255)                                                           aCAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCa                                                                              <  2:612607/140‑1 (MQ=255)                                                                 aTTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGc                                                                        >  1:1187250/1‑140 (MQ=255)                                                                         ttAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTggcg                                                                <  1:892255/140‑1 (MQ=255)                                                                           aGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTggcggc                                                              >  2:670308/1‑140 (MQ=255)                                                                            gCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGgcggcg                                                             <  2:646572/140‑1 (MQ=255)                                                                            gCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGgcggcg                                                             <  1:1112634/140‑1 (MQ=255)                                                                             cGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGa                                                            >  2:104282/1‑140 (MQ=255)                                                                             cGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGa                                                            <  1:313131/140‑1 (MQ=255)                                                                             cGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGa                                                            >  2:246485/1‑140 (MQ=255)                                                                              gACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAg                                                           <  1:1152359/140‑1 (MQ=255)                                                                              gACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAg                                                           <  2:1136009/140‑1 (MQ=255)                                                                              gACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAg                                                           <  2:696112/140‑1 (MQ=255)                                                                               aCATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGc                                                          <  1:120610/140‑1 (MQ=255)                                                                                 aTCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGTAATGTATGGCATGTCGCTGGTggcg                                                                <  2:1007413/132‑1 (MQ=255)                                                                                 aTCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGTAATGTATGGCATGTCGCTGGTggcg                                                                >  1:1007413/1‑132 (MQ=255)                                                                                   ccTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGTCATGTCGCTGGTGGCGGCGAGCTACa                                                      <  2:287274/140‑1 (MQ=255)                                                                                     tGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTggcggc                                                              <  2:533408/130‑1 (MQ=255)                                                                                     tGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTggcggc                                                              >  1:533408/1‑130 (MQ=255)                                                                                     tGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATg                                                    <  1:246485/140‑1 (MQ=255)                                                                                       gCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAg                                                           <  2:574953/131‑1 (MQ=255)                                                                                       gCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAg                                                           >  1:574953/1‑131 (MQ=255)                                                                                            tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATg                                                    <  1:4678/133‑1 (MQ=255)                                                                                            tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATg                                                    >  2:1101570/1‑133 (MQ=255)                                                                                            tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATg                                                    <  1:1101570/133‑1 (MQ=255)                                                                                            tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATg                                                    >  2:4678/1‑133 (MQ=255)                                                                                              tGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATtgtg                                           <  2:280639/140‑1 (MQ=255)                                                                                                     cAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATcaaca                                    >  2:562944/1‑140 (MQ=255)                                                                                                        caccacCTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACaaaa                                 >  1:245434/1‑140 (MQ=255)                                                                                                             ccTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACGAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTc                            >  2:823721/1‑140 (MQ=255)                                                                                                             ccTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTc                            >  2:648901/1‑140 (MQ=255)                                                                                                             ccTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTc                            >  2:345767/1‑140 (MQ=255)                                                                                                             ccTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTc                            >  2:674278/1‑140 (MQ=255)                                                                                                             ccTGGTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTc                            >  2:731912/1‑140 (MQ=255)                                                                                                             ccTGGTATTGCAGCATGGCGATTTTTGGCATCTTCACCCTCTACGCCATTCTGAGCTACTCCCCCACCTATCTGACCGAAACGTATGGCATGTCGCTGGCGGCGGCCAGCCACCTGGGGATTGTGATCAACAAAATATTc                            >  2:691547/1‑140 (MQ=255)                                                                                                                 gTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcg                        <  2:82775/140‑1 (MQ=255)                                                                                                                 gTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcg                        <  2:188146/140‑1 (MQ=255)                                                                                                                 gTATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCAATTCTGAGTTACTCCACCAACTATCTGACCGACATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcg                        <  2:1119159/140‑1 (MQ=255)                                                                                                                  tATTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcgc                       >  1:152340/1‑140 (MQ=255)                                                                                                                  tATTGCAGCATGGTGATTTTTCGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcgc                       >  1:346226/1‑140 (MQ=255)                                                                                                                   aTTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcgcg                      <  2:56386/140‑1 (MQ=255)                                                                                                                   aTTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcgcg                      <  1:345767/140‑1 (MQ=255)                                                                                                                   aTTGCAGCATGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCcgcgcg                      <  1:674278/140‑1 (MQ=255)                                                                                                                       cagcaTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCtgt                  >  2:561876/1‑140 (MQ=255)                                                                                                                       cagcaTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCtgt                  >  2:349088/1‑140 (MQ=255)                                                                                                                       cagcaTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCtgt                  >  2:78214/1‑140 (MQ=255)                                                                                                                        agcaTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCtgtg                 >  2:981369/1‑140 (MQ=255)                                                                                                                        agcaTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCtgtg                 >  1:366716/1‑140 (MQ=255)                                                                                                                        agcaTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCtgtg                 <  1:558822/140‑1 (MQ=255)                                                                                                                          caTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCg               >  2:70824/1‑140 (MQ=255)                                                                                                                          caTGGCGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCg               >  2:710025/1‑140 (MQ=255)                                                                                                                           aTGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGGTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGCGCTACATGGGGATTGTGATCGACAAAATATTCCGCGCGCTGTGCgg              >  2:123282/1‑140 (MQ=255)                                                                                                                            tGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGc             >  2:827871/1‑140 (MQ=255)                                                                                                                            tGGTGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGc             >  2:516040/1‑140 (MQ=255)                                                                                                                               tGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGCCCa          >  1:478166/1‑140 (MQ=255)                                                                                                                               tGATTTTTGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATAAACAAAATATTCCGCGCGCTGTGCGGCCCa          >  1:559438/1‑140 (MQ=255)                                                                                                                                    tttGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGCCCACTTgg     <  2:395142/140‑1 (MQ=255)                                                                                                                                    tttGGCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGCCCACTTgg     <  2:909789/140‑1 (MQ=255)                                                                                                                                       ggCATCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGCCCACTTggcgg  <  2:267988/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAGTTACTCCACCAACTATCTGACCGAAATGTATGGCATGTCGCTGGTGGCGGCGAGCTACATGGGGATTGTGATCAACAAAATATTCCGCGCGCTGTGCGGCCCACTTGGCGG  >  NC_000913/2901151‑2901424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑