Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,265,334	C→T	G232D (GGC→GAC)	tdcB ←	catabolic threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,265,334	0	C	T	100.0%	279.6 / NA	83	G232D (GGC→GAC)	tdcB	catabolic threonine dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/45);  total (38/45)

ATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAAC  >  NC_000913/3265198‑3265469                                                                                                                                         |                                                                                                                                        aTTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCgg                                                                                                                                      <  2:922482/140‑1 (MQ=255) aTTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCgg                                                                                                                                      >  1:603166/1‑140 (MQ=255)      gcTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGtt                                                                                                                                 <  2:508258/140‑1 (MQ=255)      gcTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGtt                                                                                                                                 <  1:1198598/140‑1 (MQ=255)       cTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACTATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTc                                                                                                                                >  1:968316/1‑140 (MQ=255)       cTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTc                                                                                                                                >  2:934740/1‑140 (MQ=255)        tGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCg                                                                                                                               <  2:1136949/140‑1 (MQ=255)          aaTTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACTATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGt                                                                                                                             <  2:968316/140‑1 (MQ=255)          aaTTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGt                                                                                                                             >  1:72029/1‑140 (MQ=255)           aTTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTg                                                                                                                            <  2:345490/140‑1 (MQ=255)                 gCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGt                                                                                                                      <  1:1045066/140‑1 (MQ=255)                 gCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGt                                                                                                                      <  2:498398/140‑1 (MQ=255)                   aaTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTa                                                                                                                    <  2:1026273/140‑1 (MQ=255)                    aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGt                                                                                                                         <  2:878497/134‑1 (MQ=255)                    aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGt                                                                                                                         >  1:878497/1‑134 (MQ=255)                    aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTAt                                                                                                                   >  1:1144227/1‑140 (MQ=255)                    aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTAt                                                                                                                   >  1:490621/1‑140 (MQ=255)                          cTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTcc                                                                                                             >  1:1092654/1‑140 (MQ=255)                          cTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTcc                                                                                                             <  2:325096/140‑1 (MQ=255)                          cTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTcc                                                                                                             <  1:745685/140‑1 (MQ=255)                           tGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                            <  2:1027521/140‑1 (MQ=255)                           tGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                            <  2:1144227/140‑1 (MQ=255)                           tGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                            <  2:867077/140‑1 (MQ=255)                           tGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCg                                                                                                            >  1:692510/1‑140 (MQ=255)                             tttCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa                                                                                                          >  1:523068/1‑140 (MQ=255)                             tttCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa                                                                                                          >  1:1030363/1‑140 (MQ=255)                               tCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa                                                                                                          <  1:321594/138‑1 (MQ=255)                               tCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa                                                                                                          >  2:321594/1‑138 (MQ=255)                               tCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAg                                                                                                         <  2:343076/139‑1 (MQ=255)                               tCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAg                                                                                                         >  1:343076/1‑139 (MQ=255)                               tCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGt                                                                                                        >  1:105051/1‑140 (MQ=255)                               tCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGCGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGt                                                                                                        >  1:168373/1‑140 (MQ=255)                                  gATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGa                                                                                                     <  2:105051/140‑1 (MQ=255)                                   aTTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa                                                                                                          <  2:1043438/134‑1 (MQ=255)                                   aTTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa                                                                                                          >  1:1043438/1‑134 (MQ=255)                                   aTTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGaa                                                                                                    >  1:719411/1‑140 (MQ=255)                                      tCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAaaga                                                                                                 <  2:603166/140‑1 (MQ=255)                                       cGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAaagaa                                                                                                >  2:234795/1‑140 (MQ=255)                                        gTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGATACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAaagaag                                                                                               <  2:1092654/140‑1 (MQ=255)                                             cGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAgccgcc                                                                                          <  1:369200/140‑1 (MQ=255)                                                  aCCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGcc                                                                                     >  1:1108911/1‑140 (MQ=255)                                                       cACGATGTCTTCTGATAATTCACGAACGATTTCGTAAGTCAAATTCCCGGGGCGGGAGCCATCACACCCATCCCCCAGGGTGTCGGTAGTTCGGTGGGTGGTTATTTCCCCGGAGTGGAAAGAAGCCGCCATGCCGTGaa                                                                                <  2:719411/140‑1 (MQ=255)                                                       cACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTgta                                                                                >  2:817475/1‑138 (MQ=255)                                                       cACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGaa                                                                                <  1:436977/140‑1 (MQ=255)                                                            tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAgccgcc                                                                                          <  2:109044/125‑1 (MQ=255)                                                            tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAgccgcc                                                                                          >  1:109044/1‑125 (MQ=255)                                                            tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGttt                                                                           >  2:383984/1‑140 (MQ=255)                                                            tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGttt                                                                           >  1:293619/1‑140 (MQ=255)                                                            tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGttt                                                                           >  1:249736/1‑140 (MQ=255)                                                              tCATCGACTAATTCACGAACGGTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCAATGCCGTGAACGTTTTc                                                                         >  2:812504/1‑140 (MQ=255)                                                               cATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa                                                                        <  1:633036/140‑1 (MQ=255)                                                               cATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCCCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa                                                                        <  2:251256/140‑1 (MQ=255)                                                                aTCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGaa                                                                                <  2:394683/131‑1 (MQ=255)                                                                aTCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGaa                                                                                >  1:394683/1‑131 (MQ=255)                                                                 tCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGa                                                                      <  1:817475/140‑1 (MQ=255)                                                                  cGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGAc                                                                     <  2:249736/140‑1 (MQ=255)                                                                    aCTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGCCATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTg                                                                   <  2:523068/140‑1 (MQ=255)                                                                    aCTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTg                                                                   <  1:518833/140‑1 (MQ=255)                                                                      tAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAGAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTa                                                                 >  1:540486/1‑140 (MQ=255)                                                                      tAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTa                                                                 >  1:272917/1‑140 (MQ=255)                                                                      tAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTa                                                                 >  1:336353/1‑140 (MQ=255)                                                                        aTTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTa                                                                 >  2:215545/1‑138 (MQ=255)                                                                        aTTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTa                                                                 <  1:215545/138‑1 (MQ=255)                                                                        aTTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACg                                                               >  2:498315/1‑140 (MQ=255)                                                                          tCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGcc                                                             <  1:25209/140‑1 (MQ=255)                                                                          tCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGcc                                                             <  2:540486/140‑1 (MQ=255)                                                                           cACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCa                                                            <  1:879793/140‑1 (MQ=255)                                                                             cgaacgaTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAAt                                                          <  1:667492/140‑1 (MQ=255)                                                                                 cgaTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAaca                                                      >  1:605436/1‑140 (MQ=255)                                                                                   aTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACg                                                    <  1:1143343/140‑1 (MQ=255)                                                                                   aTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACg                                                    >  2:119118/1‑140 (MQ=255)                                                                                   aTTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACg                                                    >  2:1143343/1‑140 (MQ=255)                                                                                     ttCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGaa                                                  <  2:293619/140‑1 (MQ=255)                                                                                        gTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATgg                                               <  1:412872/140‑1 (MQ=255)                                                                                         tAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGt                                              <  1:454443/140‑1 (MQ=255)                                                                                         tAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGt                                              <  2:336353/140‑1 (MQ=255)                                                                                         tAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGt                                              <  2:1030363/140‑1 (MQ=255)                                                                                         tAAGTGAAGTTTCCCGGGGCAGGGACATCACACCCTGCCGCCAGGTGGTGGGTAGTTCGGTGCGTGGTTATTCGCCCGGTGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGt                                              <  2:622003/140‑1 (MQ=255)                                                                                                aaTTACCCGGGCGGGGGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTa                                       <  2:567896/140‑1 (MQ=255)                                                                                                      ccGGGCGGGAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGAt                                 <  2:605436/140‑1 (MQ=255)                                                                                                         ggggggAGACATCAAACCCACCCGCAAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCGGGGGTGGAAAGAGGCCGCCATGCCGTGACCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTa                              <  2:272917/137‑1 (MQ=255)                                                                                                         ggcgggAGACATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTa                              >  1:188964/1‑140 (MQ=255)                                                                                                              gagaCATCACAACCATCCGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGc                         <  1:1134453/140‑1 (MQ=255)                                                                                                                     cacaACCATCCGCCAGGGTGTCGGTAGTTCGTTCGGTGGTTATTTCTCCGGAGTGAAAAGAAGCCGCCATGCCGTTAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCa                  <  2:168373/140‑1 (MQ=255)                                                                                                                               cGCCAGGGTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCaa        >  1:870188/1‑140 (MQ=255)                                                                                                                                    gggTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTaaa   >  1:998182/1‑140 (MQ=255)                                                                                                                                     ggTGTCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAAc  >  1:533627/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        ATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAAC  >  NC_000913/3265198‑3265469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑