Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,467,721	T→C	E711E (GAA→GAG)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,467,721	0	T	C	100.0%	339.4 / NA	90	E711E (GAA→GAG)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/42);  total (48/42)

CTCAGGACATTCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCGGAGCG  >  NC_000913/3467596‑3467856                                                                                                                              |                                                                                                                                        cTCAGGACATTCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTg                                                                                                                           >  1:491173/1‑140 (MQ=255) cTCAGGACATTCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTg                                                                                                                           >  1:52085/1‑140 (MQ=255) cTCAGGACATTCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTg                                                                                                                           <  1:557505/140‑1 (MQ=255)      gACATTCATCCCTTCCGGCGTCGGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCg                                                                                                                      <  2:981448/140‑1 (MQ=255)           tCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAAcc                                                                                                                 >  1:803050/1‑140 (MQ=255)           tCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAAcc                                                                                                                 >  2:673306/1‑140 (MQ=255)           tCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATCTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAAcc                                                                                                                 >  1:676277/1‑140 (MQ=255)           tCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGGGTACCACATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAAcc                                                                                                                 >  2:704731/1‑140 (MQ=255)              tCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAg                                                                                                              <  2:285468/140‑1 (MQ=255)              tCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAg                                                                                                              >  1:376573/1‑140 (MQ=255)              tCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAg                                                                                                              >  1:55762/1‑140 (MQ=255)              tCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAg                                                                                                              >  1:1111125/1‑140 (MQ=255)                ccTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGt                                                                                                            >  1:479214/1‑140 (MQ=255)                ccTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGt                                                                                                            >  1:349864/1‑140 (MQ=255)                  ttCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGc                                                                                                          >  2:1141991/1‑140 (MQ=255)                   tCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGcc                                                                                                         >  1:422028/1‑140 (MQ=255)                   tCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGcc                                                                                                         <  1:1114581/140‑1 (MQ=255)                   tCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGcc                                                                                                         <  2:491173/140‑1 (MQ=255)                     cGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCtt                                                                                                       <  2:479214/140‑1 (MQ=255)                     cGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCtt                                                                                                       >  1:412690/1‑140 (MQ=255)                       gCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCg                                                                                                     <  1:984277/140‑1 (MQ=255)                       gCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCg                                                                                                     <  1:882355/140‑1 (MQ=255)                            aGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATc                                                                                                >  1:311533/1‑140 (MQ=255)                            aGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATc                                                                                                >  1:753165/1‑140 (MQ=255)                             ggCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCg                                                                                               <  1:312547/140‑1 (MQ=255)                             ggCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCg                                                                                               <  2:311533/140‑1 (MQ=255)                             ggCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCg                                                                                               <  2:422028/140‑1 (MQ=255)                             ggCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCg                                                                                               <  1:1141991/140‑1 (MQ=255)                               ccAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGaa                                                                                             >  1:1017975/1‑140 (MQ=255)                                          aaTTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACtt                                                                                  <  2:753165/140‑1 (MQ=255)                                           aTTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATCTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACttt                                                                                 <  2:676277/140‑1 (MQ=255)                                             tGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCTATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACtt                                                                                  <  2:542184/137‑1 (MQ=255)                                             tGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACtt                                                                                  >  1:542184/1‑137 (MQ=255)                                             tGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGa                                                                               >  1:1086495/1‑140 (MQ=255)                                               gCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAgg                                                                             <  2:376573/140‑1 (MQ=255)                                               gCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAgg                                                                             >  1:245874/1‑140 (MQ=255)                                               gCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAgg                                                                             <  2:1111125/140‑1 (MQ=255)                                                 aaGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGtt                                                                           <  2:1086495/140‑1 (MQ=255)                                                    gtgtACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAg                                                                        <  2:649980/140‑1 (MQ=255)                                                     tgtACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAgg                                                                       <  1:1053876/140‑1 (MQ=255)                                                             aTAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAAc                                                               <  1:992432/140‑1 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTTATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              >  2:795337/1‑140 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGtt                                                                           >  2:428409/1‑127 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGtt                                                                           <  1:428409/127‑1 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAAc                                                               <  1:142765/139‑1 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAAc                                                               >  2:142765/1‑139 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              >  1:321239/1‑140 (MQ=255)                                                              tAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              >  1:573334/1‑140 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCATGACTTTGAGGTTCAGGTTATCAACGa                                                             >  1:844016/1‑140 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGtt                                                                           >  2:359891/1‑126 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGtt                                                                           <  1:359891/126‑1 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              <  2:165590/139‑1 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              >  1:165590/1‑139 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             <  1:339171/140‑1 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             >  1:215505/1‑140 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             >  1:511222/1‑140 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             >  2:718467/1‑140 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             >  2:1013370/1‑140 (MQ=255)                                                               aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             >  2:729656/1‑140 (MQ=255)                                                                gCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGAt                                                            <  1:704731/140‑1 (MQ=255)                                                                  aaTATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGt                                                          <  1:795337/140‑1 (MQ=255)                                                                          tGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             >  1:415487/1‑129 (MQ=255)                                                                          tGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGa                                                             <  2:415487/129‑1 (MQ=255)                                                                           gCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              >  2:783993/1‑127 (MQ=255)                                                                           gCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACg                                                              <  1:783993/127‑1 (MQ=255)                                                                           gCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATaataat                                                 >  1:36189/1‑140 (MQ=255)                                                                            ccTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAAtg                                                <  2:321239/140‑1 (MQ=255)                                                                            ccTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAAtg                                                <  1:673306/140‑1 (MQ=255)                                                                            ccTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAAtg                                                <  2:245874/140‑1 (MQ=255)                                                                                cTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAAtgctgc                                            >  1:924115/1‑140 (MQ=255)                                                                                      tCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGa                                      <  2:381451/140‑1 (MQ=255)                                                                                      tCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGa                                      >  1:806504/1‑140 (MQ=255)                                                                                        cATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATc                                    >  1:963566/1‑140 (MQ=255)                                                                                             aTCCTGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTa                               <  2:36189/140‑1 (MQ=255)                                                                                                 tGCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGtt                           >  2:25447/1‑140 (MQ=255)                                                                                                  gCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTc                          >  1:35646/1‑140 (MQ=255)                                                                                                  gCACTTTCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTc                          >  2:44898/1‑140 (MQ=255)                                                                                                      tttCTTCACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTAc                      <  2:1017975/140‑1 (MQ=255)                                                                                                        tcttcACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAg                    >  1:402417/1‑140 (MQ=255)                                                                                                        tcttcACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAg                    >  2:909531/1‑140 (MQ=255)                                                                                                        tcttcACAGCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAg                    >  2:49132/1‑140 (MQ=255)                                                                                                                gCAAGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAagcagc            >  2:435921/1‑140 (MQ=255)                                                                                                                  aaGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGcagcca          <  2:294943/140‑1 (MQ=255)                                                                                                                  aaGGTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGcagcca          >  2:311154/1‑140 (MQ=255)                                                                                                                     gTTACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCg       <  2:963566/140‑1 (MQ=255)                                                                                                                      ttACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCgg      <  2:803050/140‑1 (MQ=255)                                                                                                                       tACGACGCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCGGa     <  1:435921/140‑1 (MQ=255)                                                                                                                        acgacgCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCGGAg    <  2:844016/140‑1 (MQ=255)                                                                                                                         cgacgCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCGGAGc   >  2:720953/1‑140 (MQ=255)                                                                                                                          gacgCTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCGGAGCg  <  2:573334/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                        CTCAGGACATTCATCCCTTCCGGCGTCAGGCCAGTCGGCAGAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAATAATGCTGCATCAGATCGTCTACGTTCTTACAGGAAGCAGCCAGCGGAGCG  >  NC_000913/3467596‑3467856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑