Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,555,899	C→T	E317E (GAG→GAA)	rtcA ←	RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,555,899	0	C	T	100.0%	245.7 / NA	72	E317E (GAG→GAA)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/38);  total (34/38)

TGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAG  >  NC_000913/3555762‑3556034                                                                                                                                          |                                                                                                                                        tGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTc                                                                                                                                       <  2:733489/140‑1 (MQ=255)    tGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTccac                                                                                                                                    <  1:1100991/140‑1 (MQ=255)    tGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTccac                                                                                                                                    <  1:806283/140‑1 (MQ=255)       aGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAAATAAACCGCACCCGCAAGAAACGTTCcaccac                                                                                                                                 >  2:285775/1‑140 (MQ=255)                ggTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATAtt                                                                                                                        >  1:720034/1‑140 (MQ=255)                  tACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGGTTCTATCAAACTAACCCGCAGCGGCAAGAAACGTTCCACCACCGCGATATTgg                                                                                                                      <  2:721485/140‑1 (MQ=255)                   aCGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGt                                                                                                                     >  1:689958/1‑140 (MQ=255)                     ggCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTca                                                                                                                   >  2:6467/1‑140 (MQ=255)                         tgtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTcagcag                                                                                                               >  2:787351/1‑140 (MQ=255)                          gtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTc                                                                                                                    <  2:63459/134‑1 (MQ=255)                          gtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTc                                                                                                                    >  1:63459/1‑134 (MQ=255)                          gtAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGa                                                                                                              >  1:41939/1‑140 (MQ=255)                               tttAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCa                                                                                                         <  1:787351/140‑1 (MQ=255)                                 tAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATg                                                                                                       >  2:1032258/1‑140 (MQ=255)                                   gCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAg                                                                                                     >  2:567051/1‑140 (MQ=255)                                     cGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAggg                                                                                                   >  1:344920/1‑140 (MQ=255)                                       gATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGAt                                                                                                 >  1:377993/1‑140 (MQ=255)                                       gATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGAt                                                                                                 >  1:245135/1‑140 (MQ=255)                                         tAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATgg                                                                                               >  1:227093/1‑140 (MQ=255)                                          aaCGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATggg                                                                                              <  1:6467/140‑1 (MQ=255)                                            cgcgCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCg                                                                                            <  2:689958/140‑1 (MQ=255)                                            cgcgCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCg                                                                                            <  1:645241/140‑1 (MQ=255)                                                    aTCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTaaa                                                                                    >  2:658629/1‑140 (MQ=255)                                                         gCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTccc                                                                               >  2:971990/1‑140 (MQ=255)                                                              cATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCcgcg                                                                          <  2:303680/140‑1 (MQ=255)                                                              cATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCcgcg                                                                          <  2:41939/140‑1 (MQ=255)                                                              cATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCcgcg                                                                          <  1:1053412/140‑1 (MQ=255)                                                                   ctGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGcgcccgc                                                                     <  1:1187475/140‑1 (MQ=255)                                                                    tGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGcgcccgcc                                                                    >  1:159534/1‑140 (MQ=255)                                                                      catcatTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAg                                                                  >  2:141563/1‑140 (MQ=255)                                                                        tcatTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAgcg                                                                <  2:503760/140‑1 (MQ=255)                                                                          atTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                              <  2:344920/140‑1 (MQ=255)                                                                          atTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                              <  1:141563/140‑1 (MQ=255)                                                                          atTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                              <  2:159534/140‑1 (MQ=255)                                                                           ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTccc                                                                               <  2:721800/122‑1 (MQ=255)                                                                           ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTccc                                                                               >  1:721800/1‑122 (MQ=255)                                                                           ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCa                                                             >  1:854766/1‑140 (MQ=255)                                                                           ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCa                                                             >  2:300652/1‑140 (MQ=255)                                                                               aTGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCgg                                                         <  2:144683/140‑1 (MQ=255)                                                                                tGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGt                                                        <  2:245135/140‑1 (MQ=255)                                                                                   tCACCCGCGTTACGCCATCTGTTTCTGTCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGc                                                     <  1:392428/140‑1 (MQ=255)                                                                                   tCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGG‑‑‑CCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCAcc                                                  >  1:46753/1‑140 (MQ=255)                                                                                    cACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCa                                                             <  2:217270/131‑1 (MQ=255)                                                                                    cACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCa                                                             >  1:217270/1‑131 (MQ=255)                                                                                    cACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCa                                                    >  2:317380/1‑140 (MQ=255)                                                                                     aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGTCAGCGCCATCGGTAGCAc                                                   >  1:907004/1‑140 (MQ=255)                                                                                     aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCAc                                                   >  1:34237/1‑140 (MQ=255)                                                                                       ccGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCa                                                 >  1:578564/1‑140 (MQ=255)                                                                                          cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAg                                                <  1:317575/138‑1 (MQ=255)                                                                                          cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAg                                                >  2:317575/1‑138 (MQ=255)                                                                                            ttACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                              >  2:946933/1‑122 (MQ=255)                                                                                            ttACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGcc                                                              <  1:946933/122‑1 (MQ=255)                                                                                             tACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                           <  1:1032258/140‑1 (MQ=255)                                                                                               cGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCa                                         >  1:995353/1‑140 (MQ=255)                                                                                                  cATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCa                                      <  1:300652/140‑1 (MQ=255)                                                                                                   aTCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCaa                                     <  2:897639/140‑1 (MQ=255)                                                                                                     cTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGa                                   <  1:971990/140‑1 (MQ=255)                                                                                                     cTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGa                                   <  1:788224/140‑1 (MQ=255)                                                                                                             aTCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTcccc                           <  2:227093/140‑1 (MQ=255)                                                                                                              tCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCTTCGGTAGCACCAGTTGGTCAGCAAGATATTccccc                          >  2:833250/1‑140 (MQ=255)                                                                                                              tCAAACTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTccccc                          >  2:1154827/1‑140 (MQ=255)                                                                                                                aaaCTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAc                        >  1:990333/1‑140 (MQ=255)                                                                                                                aaaCTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAc                        <  2:854766/140‑1 (MQ=255)                                                                                                                aaaCTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAc                        <  1:551098/140‑1 (MQ=255)                                                                                                                  aCTAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccg                      >  2:5822/1‑140 (MQ=255)                                                                                                                    tAAACCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgcc                    <  1:567051/140‑1 (MQ=255)                                                                                                                     aaaCCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg                   <  1:87948/140‑1 (MQ=255)                                                                                                                      aaCCGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccgc                  >  1:994819/1‑140 (MQ=255)                                                                                                                         cGCACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGt               <  2:175767/140‑1 (MQ=255)                                                                                                                           cACCGGCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGc             <  2:720034/140‑1 (MQ=255)                                                                                                                                gCAAGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCTCCATCGGTAGCACCAGTTGGCCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGcc        >  1:635298/1‑140 (MQ=255)                                                                                                                                   aGAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGG‑‑‑CCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAg  <  2:46753/140‑1 (MQ=255)                                                                                                                                    gAAACGTTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGt    <  2:994819/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAG  >  NC_000913/3555762‑3556034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑