Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,630	(T)6→5	intergenic (‑150/+111)	ybiO ← / ← glnQ	moderate conductance mechanosensitive channel/L‑glutamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,625	0	T	.	100.0%	246.0 / NA	57	intergenic (‑145/+116)	ybiO/glnQ	moderate conductance mechanosensitive channel/L‑glutamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/30);  total (27/30)

CCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAA  >  NC_000913/845488‑845759                                                                                                                                          |                                                                                                                                       ccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCttt                                                                                                                                      >  1:832433/1‑140 (MQ=255) ccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCttt                                                                                                                                      <  2:52054/140‑1 (MQ=255)             tAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATgcg                                                                                                                         <  2:1119343/140‑1 (MQ=255)              agagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATgcgg                                                                                                                        >  1:325084/1‑140 (MQ=255)               gagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATgcggc                                                                                                                       >  2:1152855/1‑140 (MQ=255)                agCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATgcggcg                                                                                                                      >  2:944031/1‑140 (MQ=255)                 gCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGt                                                                                                                     >  1:141944/1‑140 (MQ=255)                  cAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    <  2:77831/140‑1 (MQ=255)                  cAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    <  1:944031/140‑1 (MQ=255)                  cAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    <  2:734053/140‑1 (MQ=255)                  cAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    <  2:400868/140‑1 (MQ=255)                  cAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    <  1:624251/140‑1 (MQ=255)                   aaaaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGa                                                                                                                   <  2:425769/140‑1 (MQ=255)                     aaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAAc                                                                                                                 >  1:618584/1‑140 (MQ=255)                      tcctccaGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    <  2:977684/130‑1 (MQ=255)                      tcctccaGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTg                                                                                                                    >  1:977684/7‑136 (MQ=255)                             gTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                         <  1:96285/140‑1 (MQ=255)                                  gATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                    >  1:707490/1‑140 (MQ=255)                                    tGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTa                                                                                                  <  2:736273/140‑1 (MQ=255)                                     gCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTAc                                                                                                 >  1:524991/1‑140 (MQ=255)                                       tGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                               >  2:170762/1‑140 (MQ=255)                                        ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                         <  2:835721/129‑1 (MQ=255)                                        ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                         >  1:835721/1‑129 (MQ=255)                                        ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAg                                                                                              >  1:849986/1‑140 (MQ=255)                                        ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAg                                                                                              >  2:1073752/1‑140 (MQ=255)                                        ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAg                                                                                              >  2:579861/1‑140 (MQ=255)                                        ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAg                                                                                              >  2:370146/1‑140 (MQ=255)                                              ggggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATg                                                                                        <  2:141944/140‑1 (MQ=255)                                                ggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCa                                                                                      <  1:370146/140‑1 (MQ=255)                                                           aTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAg                                                                           <  2:524991/140‑1 (MQ=255)                                                             gagaGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGtt                                                                         <  1:579861/140‑1 (MQ=255)                                                                agGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGcc                                                                      >  1:1062913/1‑140 (MQ=255)                                                                  gAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAg                                                                    <  1:396964/140‑1 (MQ=255)                                                                     tCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGata                                                                 <  2:915020/140‑1 (MQ=255)                                                                     tCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGata                                                                 <  2:453862/140‑1 (MQ=255)                                                                     tCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATATTAATAAGTTGCCAGata                                                                 <  1:1152855/140‑1 (MQ=255)                                                                         gtgtGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCt                                                             >  2:1083779/1‑140 (MQ=255)                                                                            tgCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                                                          <  2:325084/140‑1 (MQ=255)                                                                              ccTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAgg                                                        >  1:183956/1‑140 (MQ=255)                                                                               cTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGc                                                       <  1:184186/140‑1 (MQ=255)                                                                               cTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGc                                                       <  2:707490/140‑1 (MQ=255)                                                                                   cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGa                                                   >  1:80262/1‑140 (MQ=255)                                                                                    tttCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGAt                                                  <  2:183956/140‑1 (MQ=255)                                                                                        gggTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc                                              >  2:423579/1‑140 (MQ=255)                                                                                            gAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg                                          <  1:1083779/140‑1 (MQ=255)                                                                                                    ggaAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAg                                  >  2:92268/1‑140 (MQ=255)                                                                                                    ggaAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAg                                  >  1:343062/1‑140 (MQ=255)                                                                                                     gaAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCACGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAgg                                 >  1:1079245/1‑140 (MQ=255)                                                                                                       aGGGATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                               <  1:929164/140‑1 (MQ=255)                                                                                                         ggATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                             <  1:343689/140‑1 (MQ=255)                                                                                                         ggATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                             <  2:618584/140‑1 (MQ=255)                                                                                                          gATATTAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTTTCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAtg                            <  2:214794/140‑1 (MQ=255)                                                                                                               tAAGCAGGTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCt                       >  2:1069401/1‑140 (MQ=255)                                                                                                                      gTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                             <  2:1145707/127‑1 (MQ=255)                                                                                                                      gTTGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                             >  1:1145707/1‑127 (MQ=255)                                                                                                                        tGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTc                        <  2:1006500/130‑1 (MQ=255)                                                                                                                        tGCTGACAACGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTc                        >  1:1006500/1‑130 (MQ=255)                                                                                                                                aCGTGCGGGC‑TTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGtgctg      >  2:671473/1‑140 (MQ=255)                                                                                                                                    gCGGGC‑TTTTTGTCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCaaa  >  1:82805/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAA  >  NC_000913/845488‑845759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑