Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,607,885	Δ1 bp	coding (969/1581 nt)	hyfF →	hydrogenase 4 component F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,607,882	0	G	.	100.0%	249.7 / NA	58	coding (966/1581 nt)	hyfF	hydrogenase 4 component F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (31/27);  total (31/27)

AGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCA  >  NC_000913/2607750‑2608015                                                                                                                                     |                                                                                                                                      aGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGt                                                                                                                               >  1:220232/1‑140 (MQ=255)  gCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTc                                                                                                                              >  2:686983/1‑140 (MQ=255)  gCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTc                                                                                                                              >  2:925932/1‑140 (MQ=255)  gCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTc                                                                                                                              >  2:186663/1‑140 (MQ=255)  gCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTc                                                                                                                              >  1:662807/1‑140 (MQ=255)  gCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTc                                                                                                                              >  2:1006638/1‑140 (MQ=255)       cGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGt                                                                                                                         <  1:925932/140‑1 (MQ=255)        ggCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTg                                                                                                                        <  1:686983/140‑1 (MQ=255)                            gttgttGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGcc                                                                                                    >  1:238542/1‑140 (MQ=255)                              tgttgCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGc                                                                                                  >  1:309644/1‑140 (MQ=255)                                ttgCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTg                                                                                                <  2:159877/140‑1 (MQ=255)                                 tgCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTgg                                                                                               <  1:359278/140‑1 (MQ=255)                                      aTCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGG‑GTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAAttt                                                                                         >  1:129354/1‑140 (MQ=255)                                             gCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCcg                                                                                   <  2:220232/140‑1 (MQ=255)                                                  ttgtCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCgctg                                                                              <  2:511647/140‑1 (MQ=255)                                                   tgtCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCgctgc                                                                             >  2:762943/1‑140 (MQ=255)                                                      cGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTATTGCCGCGCtgctgc                                                                          >  2:546224/1‑140 (MQ=255)                                                       ggTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                         <  1:1006638/140‑1 (MQ=255)                                                       ggTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                         <  2:605447/140‑1 (MQ=255)                                                       ggTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                         <  1:320019/140‑1 (MQ=255)                                                         ttGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGcaca                                                                       <  1:186663/140‑1 (MQ=255)                                                           gCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCAGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATc                                                                     >  1:416109/1‑140 (MQ=255)                                                            ccGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCcg                                                                                   >  1:72064/1‑125 (MQ=255)                                                            ccGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCcg                                                                                   <  2:72064/125‑1 (MQ=255)                                                            ccGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCt                                                                    >  1:420004/1‑140 (MQ=255)                                                                   ggcATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCAGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACca                                                             <  2:416109/140‑1 (MQ=255)                                                                    gcATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                         <  1:814807/127‑1 (MQ=255)                                                                    gcATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                         >  2:814807/1‑127 (MQ=255)                                                                       tttttCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGt                                                         <  2:420004/140‑1 (MQ=255)                                                                       tttttCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGt                                                         <  1:762943/140‑1 (MQ=255)                                                                         tttCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCt                                                       <  1:347318/140‑1 (MQ=255)                                                                                 tGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGTATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCaaaaa                                               <  2:777593/140‑1 (MQ=255)                                                                                 tGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCaaaaa                                               <  2:1084141/140‑1 (MQ=255)                                                                                      cAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACgctg                                          >  2:801293/1‑140 (MQ=255)                                                                                           ggACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGtt                                     >  2:519115/1‑140 (MQ=255)                                                                                             aCATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACgctg                                          >  1:364394/1‑133 (MQ=255)                                                                                             aCATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACgctg                                          <  2:364394/133‑1 (MQ=255)                                                                                             aCATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCt                                   >  1:1039790/1‑140 (MQ=255)                                                                                             aCATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCt                                   >  1:861813/1‑140 (MQ=255)                                                                                                ttAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCg                                >  1:405786/1‑140 (MQ=255)                                                                                                  aaGCGTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGt                              <  1:1050577/140‑1 (MQ=255)                                                                                                      gTCTGCTGGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCg                          >  2:828056/1‑140 (MQ=255)                                                                                                        ctgctgGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGc                        >  2:547328/1‑140 (MQ=255)                                                                                                        ctgctgGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGc                        >  1:209226/1‑140 (MQ=255)                                                                                                         tgctgGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCa                       <  1:1135445/140‑1 (MQ=255)                                                                                                         tgctgGCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCa                       <  1:6164/140‑1 (MQ=255)                                                                                                              gCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCAGGCAATGTa                  <  2:405786/140‑1 (MQ=255)                                                                                                              gCGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTATTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTa                  <  1:546224/140‑1 (MQ=255)                                                                                                               cGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGGTGTTCTGCGGTTCCGGCAATGTAc                 <  2:662807/140‑1 (MQ=255)                                                                                                               cGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTAc                 <  2:116056/140‑1 (MQ=255)                                                                                                               cGTACTCCAGCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTAc                 <  1:828056/140‑1 (MQ=255)                                                                                                                        gCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATg                    <  2:937035/128‑1 (MQ=255)                                                                                                                        gCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATg                    >  1:937035/1‑128 (MQ=255)                                                                                                                        gCGTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGt        >  1:3100/1‑140 (MQ=255)                                                                                                                          gTGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTAc      >  2:913048/1‑140 (MQ=255)                                                                                                                           tGGAGAACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACg     >  2:850878/1‑140 (MQ=255)                                                                                                                              agaACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCa  >  2:1101646/1‑140 (MQ=255)                                                                                                                              agaACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCa  >  2:461661/1‑140 (MQ=255)                                                                                                                              agaACAT‑GGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCa  >  2:404585/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      AGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCA  >  NC_000913/2607750‑2608015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑