Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,905,561	(C)5→6	coding (37/1878 nt)	bglF ←	beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,905,556	1	.	C	100.0%	330.7 / NA	91	G14G (GGC→GGG)	bglF	beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (60/31);  total (60/31)

CCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCG‑CCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCC  >  NC_000913/3905417‑3905690                                                                                                                                             |                                                                                                                                        ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg                                                                                                                                          >  2:42203/1‑140 (MQ=255) ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg                                                                                                                                          >  1:22606/1‑140 (MQ=255) ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg                                                                                                                                          <  2:841447/140‑1 (MQ=255) ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg                                                                                                                                          >  1:1126937/1‑140 (MQ=255)  cTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg‑c                                                                                                                                        >  2:137942/1‑140 (MQ=255)  cTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg‑c                                                                                                                                        >  1:726072/1‑140 (MQ=255)  cTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg‑c                                                                                                                                        >  2:1040490/1‑140 (MQ=255)  cTGAAACTGGCCACCGCTTTCCACCACCACAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTgcg‑c                                                                                                                                        >  1:63641/1‑140 (MQ=255)   tGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCG‑cc                                                                                                                                       <  2:568728/140‑1 (MQ=255)            ccACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTc                                                                                                                               >  1:148287/1‑140 (MQ=255)            ccACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACATAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTc                                                                                                                               >  1:544070/1‑140 (MQ=255)                  cTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGa                                                                                                                         >  1:53248/1‑140 (MQ=255)                          caccaTAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTAttttt                                                                                                                 <  2:512609/140‑1 (MQ=255)                             caTAATAATACGGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTg                                                                                                              >  1:355267/1‑140 (MQ=255)                                  taataCCGGGGGTCTTTTTCAGTACCGCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTaa                                                                                                         <  2:13199/140‑1 (MQ=255)                                   aataCGGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAAc                                                                                                        <  2:355267/140‑1 (MQ=255)                                    ataCCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTAttttt                                                                                                                 <  1:704145/130‑1 (MQ=255)                                    ataCCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTAttttt                                                                                                                 >  2:704145/1‑130 (MQ=255)                                    ataCCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACt                                                                                                       <  2:421420/140‑1 (MQ=255)                                    ataCCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACt                                                                                                       >  2:798574/1‑140 (MQ=255)                                    ataCCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACt                                                                                                       <  2:940211/140‑1 (MQ=255)                                    ataCCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTT‑AAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTc                                                                                                      >  1:231404/1‑140 (MQ=255)                                       ccGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCg                                                                                                    >  2:712491/1‑140 (MQ=255)                                       ccGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCg                                                                                                    >  1:675187/1‑140 (MQ=255)                                       ccGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCg                                                                                                    >  1:565955/1‑140 (MQ=255)                                        cGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGt                                                                                                   >  2:342140/1‑140 (MQ=255)                                        cGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGt                                                                                                   <  2:148287/140‑1 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACt                                                                                                       >  2:486705/1‑134 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACt                                                                                                       <  1:486705/134‑1 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTc                                                                                                  >  2:636301/1‑139 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTc                                                                                                  <  1:636301/139‑1 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  1:936000/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  1:94388/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  1:914404/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  2:676644/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  2:681675/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  1:157400/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  1:1100691/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  2:893606/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCa                                                                                                 >  2:949644/1‑140 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACATAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAAc                                                                                                        >  2:1016117/1‑133 (MQ=255)                                          ggggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACATAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAAc                                                                                                        <  1:1016117/133‑1 (MQ=255)                                           gggTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCAt                                                                                                <  1:253843/140‑1 (MQ=255)                                              tCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAAc                                                                                             <  1:676644/140‑1 (MQ=255)                                              tCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAAc                                                                                             <  1:712491/140‑1 (MQ=255)                                                tttttCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAAc                                                                                                        >  2:246764/1‑127 (MQ=255)                                                tttttCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAAc                                                                                                        <  1:246764/127‑1 (MQ=255)                                                tttttCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACtt                                                                                           >  1:756916/1‑140 (MQ=255)                                                tttttCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCACCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACtt                                                                                           >  1:941164/1‑140 (MQ=255)                                                 ttttCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTg                                                                                          <  1:1040490/140‑1 (MQ=255)                                                        tACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTa                                                                                   >  1:314541/1‑140 (MQ=255)                                                        tACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTa                                                                                   >  1:617544/1‑140 (MQ=255)                                                          ccTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTAcc                                                                                 >  1:930219/1‑140 (MQ=255)                                                          ccTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTAcc                                                                                 >  2:515260/1‑140 (MQ=255)                                                          ccTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTAcc                                                                                 >  1:12632/1‑140 (MQ=255)                                                             ctGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCt                                                                              >  2:532956/1‑140 (MQ=255)                                                             ctGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCt                                                                              >  2:305429/1‑140 (MQ=255)                                                              tGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTTCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCtt                                                                             >  1:371909/1‑140 (MQ=255)                                                              tGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCtt                                                                             <  2:53248/140‑1 (MQ=255)                                                                cTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTg                                                                           >  2:1083663/1‑140 (MQ=255)                                                                cTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTg                                                                           >  2:34021/1‑140 (MQ=255)                                                                      ctttgctttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCaa                                                                     <  1:137942/140‑1 (MQ=255)                                                                        ttgctttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCaaaa                                                                   <  2:675187/140‑1 (MQ=255)                                                                        ttgctttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCaaaa                                                                   >  1:397732/1‑140 (MQ=255)                                                                            tttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTcc                                                               <  2:268852/140‑1 (MQ=255)                                                                            tttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGACTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTcc                                                               <  1:1054666/140‑1 (MQ=255)                                                                               cATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGCCTCCTGCGACATTTTTTCTGCCTAACTCCGCCATACCTTGCCCTCTACCCCTTTGCGCAAAAACTCC‑aaa                                                           >  2:196049/1‑140 (MQ=255)                                                                                 tCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑aaaaa                                                         >  2:276766/1‑140 (MQ=255)                                                                                 tCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑aaaaa                                                         >  1:109633/1‑140 (MQ=255)                                                                                     ttaatttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑aaaaaaaaa                                                     >  1:697313/1‑140 (MQ=255)                                                                                     ttaatttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑aaaaaaaaa                                                     >  2:1076131/1‑140 (MQ=255)                                                                                              aaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGaaaaa                                            >  1:666717/1‑140 (MQ=255)                                                                                                   aaTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACgg                                       >  2:774857/1‑140 (MQ=255)                                                                                                    aTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGc                                      <  2:94388/140‑1 (MQ=255)                                                                                                    aTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGc                                      <  2:544070/140‑1 (MQ=255)                                                                                                    aTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGc                                      <  2:12632/140‑1 (MQ=255)                                                                                                        cgTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGa                                  >  1:914788/1‑140 (MQ=255)                                                                                                          ttGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACg                                >  1:518897/1‑140 (MQ=255)                                                                                                                 aTGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCa                         <  1:515260/140‑1 (MQ=255)                                                                                                                  tGCATCAGACTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCaa                        >  2:174461/1‑140 (MQ=255)                                                                                                                          aCTCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtttt                >  2:893754/1‑140 (MQ=255)                                                                                                                            tCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtttttt              >  2:513487/1‑140 (MQ=255)                                                                                                                            tCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtttttt              <  1:305429/140‑1 (MQ=255)                                                                                                                            tCACAATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtttttt              >  1:17030/1‑140 (MQ=255)                                                                                                                              acaATGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTc            >  1:618938/1‑140 (MQ=255)                                                                                                                                  tGTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGt        >  2:620715/1‑140 (MQ=255)                                                                                                                                   gTTATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGtt       >  1:573926/1‑140 (MQ=255)                                                                                                                                    ttATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCAAAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGtt       >  1:179512/1‑140 (MQ=255)                                                                                                                                    ttATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGttt      <  2:518897/140‑1 (MQ=255)                                                                                                                                    ttATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGttt      >  1:33500/1‑140 (MQ=255)                                                                                                                                    ttATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGttt      >  1:435825/1‑140 (MQ=255)                                                                                                                                    ttATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGttt      >  1:77569/1‑140 (MQ=255)                                                                                                                                    ttATCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGttt      >  1:820589/1‑140 (MQ=255)                                                                                                                                      aTCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTg    <  2:208934/140‑1 (MQ=255)                                                                                                                                      aTCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTg    >  2:80485/1‑140 (MQ=255)                                                                                                                                      aTCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTg    >  1:1094538/1‑140 (MQ=255)                                                                                                                                       tCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGc   <  2:756916/140‑1 (MQ=255)                                                                                                                                       tCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGc   <  2:63641/140‑1 (MQ=255)                                                                                                                                       tCTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGc   <  1:620715/140‑1 (MQ=255)                                                                                                                                        cTGCGCCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGcc  <  2:77569/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        CCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCG‑CCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCC  >  NC_000913/3905417‑3905690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑