Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	91.7%	112.5 / 3.6	36	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (17/16);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

AAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑GGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCA  >  NC_000913/4606071‑4606343                                                                                                                                           |                                                                                                                                           aaGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg                                                                                                                                         >  1:974448/1‑140 (MQ=255) aaGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg                                                                                                                                         <  1:777814/140‑1 (MQ=255)   gATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg‑g‑g                                                                                                                                     <  2:983360/140‑1 (MQ=255)     tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg‑g‑ggg                                                                                                                                   <  2:890494/140‑1 (MQ=255)        ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg‑g‑ggggga                                                                                                                                >  1:981903/1‑138 (MQ=255)        ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg‑g‑ggggga                                                                                                                                >  1:668144/1‑138 (MQ=255)              cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGTTGC‑GAGGGG‑G‑GGGGACTTGaa                                                                                                                           >  2:1110584/1‑140 (MQ=255)                  gggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAAcccc                                                                                                                       <  1:234409/140‑1 (MQ=255)                   ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAAccccc                                                                                                                      <  1:144481/140‑1 (MQ=255)                      gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACg                                                                                                                   <  2:619699/140‑1 (MQ=255)                      gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACg                                                                                                                   >  2:466994/1‑140 (MQ=255)                       ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg‑g‑gggggac                                                                                                                               <  1:283458/126‑4 (MQ=255)                       ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑ggg‑g‑gggggac                                                                                                                               >  2:283458/1‑123 (MQ=255)                        gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTc                                                                                                                 <  2:238150/140‑1 (MQ=255)                           ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGt                                                                                                              <  1:609887/140‑1 (MQ=255)                            tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGACCGTa                                                                                                             >  2:258366/1‑140 (MQ=255)                             gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTaa                                                                                                            <  1:993043/140‑1 (MQ=255)                              aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAg                                                                                                           <  1:466994/140‑1 (MQ=255)                              aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGACCGTAAg                                                                                                           <  1:258366/140‑1 (MQ=255)                              aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑GGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGa                                                                                                          >  2:358703/1‑140 (MQ=255)                                      cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                   <  2:981903/140‑1 (MQ=255)                                                  cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                       <  2:668144/140‑1 (MQ=255)                                                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTATTTGATATCTCCTCGTTTGGTGC‑GA‑GGGTG‑GGGGCCTTTAACCCCCACGTCCGTAAGAACAGTAACACCTGAAGCTaa                                                                                      >  2:1044009/1‑139 (MQ=25)                                                       acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGCCTTGAACCCCCACGTCCGTATACACACTAACAGCTGACGCTGAcgcg                                                                                  >  2:147509/1‑140 (MQ=37)                                                          ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCTCCTCGTTTGGTGC‑GA‑GGG‑G‑GGGGACTTGCACCCCCCCGTCCGTAAGAACACTAGCACCTGACGCATACGCGTCTa                                                                              >  2:888194/1‑140 (MQ=37)                                                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                          >  1:1013656/1‑140 (MQ=255)                                                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCGCCTCGCGTGGGGC‑GA‑GGG‑GAGGGGCCTTGAACCCCCCCCCCCGTAAGAACCCTAACACCAGAAGCTAGCGCGTCTCCCaa                                                                          >  2:269548/1‑140 (MQ=25)                                                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                      >  1:83177/1‑140 (MQ=255)                                                                   aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                      >  1:876195/1‑140 (MQ=255)                                                                    gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                     >  2:1048627/1‑140 (MQ=255)                                                                     cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                    <  1:1048627/140‑1 (MQ=255)                                                                              cAATTCCGCCACCTTCGAACAATCATATTACTTTTTTTGATATCGCCTCGTTTGGGGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                           <  2:27390/140‑1 (MQ=21)                                                                               aaTTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                          <  1:1099506/140‑1 (MQ=255)                                                                                aTTCCGGCGCCTTCGCACAGTGATCTTACTTTTTTTGATATCGCCTCGTTTGGTGG‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                         <  2:119081/140‑1 (MQ=21)                                                                                    cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                     <  2:876195/140‑1 (MQ=255)                                                                                     gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCACGTTTGGTGC‑GAGGGG‑G‑GGGGACTTAAACCCCCACGTCCGTAAGAACACTAAAACCGGAAGCCAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTga                                                    >  2:437682/1‑139 (MQ=21)                                                                                      gcgccTTCGCACAGTCAACTTACTATGTTTGATATCGACTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                   <  2:516225/137‑1 (MQ=18)                                                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                  <  1:462829/125‑1 (MQ=255)                                                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                  >  2:462829/1‑125 (MQ=255)                                                                                        gcttacacacaGTCAACTTACTTTTTTTGATATTACCGCGATTGGTGCGGG‑GGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                 <  2:205630/133‑1 (MQ=17)                                                                                             cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                            >  1:745185/1‑140 (MQ=255)                                                                                                  aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                       <  1:1110584/140‑1 (MQ=255)                                                                                                   gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACg                                      >  1:95871/1‑140 (MQ=255)                                                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                                  >  1:642048/1‑140 (MQ=255)                                                                                                           aCTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                              >  2:185551/1‑140 (MQ=255)                                                                                                            cTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                             >  1:584516/1‑140 (MQ=255)                                                                                                              ttttttGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGTGAGCAATATCAACGTGGTTTTTGGt                           >  1:634159/1‑140 (MQ=255)                                                                                                                  ttGATATCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCTCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                       >  1:118278/1‑140 (MQ=255)                                                                                                                    gATATCGCCTCG‑TTGGTGC‑GA‑GGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGATTATGGTGCGAgggg                   >  1:193285/1‑140 (MQ=18)                                                                                                                     atatCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                    <  2:1013656/140‑1 (MQ=255)                                                                                                                       atCGCCTCGTTTGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                  >  2:830849/1‑140 (MQ=255)                                                                                                                                 ttGGTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa        <  1:147509/140‑1 (MQ=255)                                                                                                                                    gTGC‑GAGGGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccc     >  2:136783/1‑140 (MQ=255)                                                                                                                                      gC‑GA‑GGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa  <  1:358703/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           AAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑GGG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCA  >  NC_000913/4606071‑4606343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑