| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 766,957 | (G)6→7 | coding (974/1977 nt) | mngA → | 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 766,951 | 1 | . | G | 98.2% | 193.8 / ‑3.2 | 55 | coding (968/1977 nt) | mngA | 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (26/28); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTAC > NC_000913/766817‑767085 | acaaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggg‑g > 1:8764/1‑140 (MQ=255) aaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggg‑ggg < 1:151108/140‑2 (MQ=255) aGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggg‑gggc < 1:419288/140‑3 (MQ=255) gcgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACCCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggg‑gggca < 1:990140/140‑4 (MQ=255) cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggg‑gggcac > 2:248695/1‑136 (MQ=255) cTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACgg > 1:59656/1‑140 (MQ=255) aCCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGc > 2:491302/1‑140 (MQ=255) cTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTgg < 1:1100843/140‑1 (MQ=255) cTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGA‑‑GGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCg < 1:19707/140‑1 (MQ=255) aaGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCgg > 1:1107560/1‑140 (MQ=255) aaGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCgg > 2:656087/1‑140 (MQ=255) aGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGt < 2:15001/140‑1 (MQ=255) aGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGt < 2:8764/140‑1 (MQ=255) cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTcg < 2:704513/140‑1 (MQ=255) cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTcg < 2:358004/140‑1 (MQ=255) gTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTcgc < 2:952753/140‑1 (MQ=255) aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCgg > 1:640839/1‑140 (MQ=255) aTGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTAtt > 2:1055263/1‑140 (MQ=255) aTGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTAtt > 2:560509/1‑140 (MQ=255) gagaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTAc > 1:514986/1‑140 (MQ=255) gagaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTAc < 2:337611/140‑1 (MQ=255) gcgTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCg < 1:1057382/140‑1 (MQ=255) aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCgg < 1:491302/140‑1 (MQ=255) cAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGc > 1:980041/1‑140 (MQ=255) gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc > 2:478082/1‑140 (MQ=255) gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc > 2:349607/1‑140 (MQ=255) tACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACaa > 2:436951/1‑140 (MQ=255) tACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGGCGCGGGATTATTGTCTCAAAACTTCTGGCTACaa > 2:559878/1‑140 (MQ=255) aCGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAg < 1:1055263/140‑1 (MQ=255) gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt < 2:59656/140‑1 (MQ=255) gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt < 2:1051279/140‑1 (MQ=255) cAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt > 1:1020234/1‑139 (MQ=255) cAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt < 2:1020234/139‑1 (MQ=255) cGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTAATGTCGCAAATACTCGGGCGACAAGATCTGtt > 2:998019/1‑140 (MQ=255) ggTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGttt > 2:509723/1‑140 (MQ=255) ggTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGAGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGGTACAAGATCTGgtt > 2:152069/1‑140 (MQ=255) gTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTa > 1:781363/1‑140 (MQ=255) gTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGGATTACTG‑CGCAAATCTTCGGGCTACAAGATCTGTTTaa > 2:586549/1‑140 (MQ=255) tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGaaaa > 2:612140/1‑140 (MQ=255) aaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc < 2:980041/140‑1 (MQ=255) aaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc < 1:560509/140‑1 (MQ=255) aaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTcc < 1:998019/140‑1 (MQ=255) aaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTcc < 2:640839/140‑1 (MQ=255) aCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCt < 2:514986/140‑1 (MQ=255) gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc < 1:248695/140‑1 (MQ=255) gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc < 2:657168/140‑1 (MQ=255) cTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCtgt < 1:42143/140‑1 (MQ=255) aaaCAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTggg > 1:774933/1‑139 (MQ=255) tGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCaa > 1:356412/1‑140 (MQ=255) tGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCt < 1:436951/140‑1 (MQ=255) aGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTgg > 2:1010807/1‑140 (MQ=255) aaTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCt > 2:584466/1‑133 (MQ=255) aaTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCt < 1:584466/133‑1 (MQ=255) tttGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGgctgc > 1:460179/1‑140 (MQ=255) ttGCCGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGgctgct < 1:1010807/140‑1 (MQ=255) cGTACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGa > 2:723732/1‑140 (MQ=255) tACCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAt > 1:259834/1‑140 (MQ=255) aCCGTTGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAtt < 1:656087/140‑1 (MQ=255) ttGATTGTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa < 2:460179/140‑1 (MQ=255) gTCGCGGGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTAc < 2:356412/140‑1 (MQ=255) | ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGG‑GGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTAC > NC_000913/766817‑767085 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |