Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,543	(C)8→9	coding (492/732 nt)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,535	1	.	C	100.0%	154.2 / NA	43	E167G (GAG→GGG)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/17);  total (26/17)

AAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATG  >  NC_000913/2734404‑2734665                                                                                                                                        |                                                                                                                                    aaaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAATGCCTCGCGA‑‑ACC‑T‑‑cccccccc                                                                                                                            <  1:407479/140‑1 (MQ=255)                      tCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGAcc                                                                                                        <  1:409652/140‑1 (MQ=255)                            gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGTGCGTGGACCAATTGc                                                                                                  <  1:285784/140‑1 (MQ=255)                            gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                                  <  1:436559/140‑1 (MQ=255)                            gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                                  <  1:420635/140‑1 (MQ=255)                                                     atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAttt                                                                         >  1:1108559/1‑140 (MQ=255)                                                         gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ATC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                                                     >  2:1011835/1‑140 (MQ=255)                                                         gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                                                     >  2:619187/1‑140 (MQ=255)                                                            aGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAt                                                                  >  2:92994/1‑140 (MQ=255)                                                                    aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa                                                          >  1:186415/1‑140 (MQ=255)                                                                     tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TCCCCCCCCCCCCTTCGAACCCCCCGGGACCAATTGCCGGCCCTAACCCGGCGGAAAAATTTTCCGGATTATCAGCa                                                          >  2:877951/1‑140 (MQ=255)                                                                       aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaaa                                                       >  2:1156833/1‑140 (MQ=255)                                                                        ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc                                                      >  1:915080/1‑140 (MQ=255)                                                                         cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaaa                                                       <  2:1021272/139‑1 (MQ=255)                                                                         cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaaa                                                       >  1:1021272/1‑139 (MQ=255)                                                                           tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                   >  1:473897/1‑140 (MQ=255)                                                                           tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                   >  2:526774/1‑140 (MQ=255)                                                                           tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                   >  2:665641/1‑140 (MQ=255)                                                                            gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                   >  1:940143/1‑140 (MQ=255)                                                                            gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                  >  1:801497/1‑140 (MQ=255)                                                                                aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                              >  2:611924/1‑140 (MQ=255)                                                                                aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                              >  2:216172/1‑140 (MQ=255)                                                                                  tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt                                            <  1:665641/140‑1 (MQ=255)                                                                                   gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtc                                           <  2:801497/140‑1 (MQ=255)                                                                                      aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct                                        <  1:216172/140‑1 (MQ=255)                                                                                      aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct                                        >  2:609774/1‑140 (MQ=255)                                                                                      aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGGctct                                        >  1:580452/1‑140 (MQ=255)                                                                                          gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCa                                    >  2:1104780/1‑140 (MQ=255)                                                                                                gCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGc                              >  1:885844/1‑140 (MQ=255)                                                                                                   ttAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTg                           >  2:593333/1‑140 (MQ=255)                                                                                                       cGTCTACTGCCATAAACGCCTCGCGA‑‑ACCGC‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                       <  1:143903/140‑1 (MQ=255)                                                                                                       cGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                       >  1:514842/1‑140 (MQ=255)                                                                                                       cGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                       <  2:915080/140‑1 (MQ=255)                                                                                                       cGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                       <  2:186415/140‑1 (MQ=255)                                                                                                       cGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                       <  1:1104780/140‑1 (MQ=255)                                                                                                        ttttACTGCCAAAAACGCCCCGCGACCCCC‑C‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                       <  1:877951/137‑1 (MQ=255)                                                                                                          cTACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAg                    >  1:1053742/1‑140 (MQ=255)                                                                                                           tACTGCCATAAACGCCTCGCGA‑‑ACC‑TCCCCCCCCCCACTTCGACCGCGCGTGGACCAATTGCCGGCCCTAACAAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAg                    >  1:549763/1‑140 (MQ=255)                                                                                                           tACTGCCATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGt                   <  2:1108559/140‑1 (MQ=255)                                                                                                                ccATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACg              <  1:128449/140‑1 (MQ=255)                                                                                                                 cATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc             <  1:636860/140‑1 (MQ=255)                                                                                                                 cATAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc             <  1:619187/140‑1 (MQ=255)                                                                                                                   tAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgcca           <  1:92994/140‑1 (MQ=255)                                                                                                                   tAAACGCCTCGCGA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgcca           >  1:280960/1‑140 (MQ=255)                                                                                                                     aaCGCCTCGCGA‑‑ACC‑TCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgccag          <  2:940143/140‑1 (MQ=255)                                                                                                                            cgcgA‑‑ACC‑TC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATg  >  2:829278/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    AAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑‑ACC‑T‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATG  >  NC_000913/2734404‑2734665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑