Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,924,165	(A)7→8	noncoding (360/369 nt)	csrB ←	small regulatory RNA CsrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,924,158	1	.	A	100.0%	135.2 / NA	38	noncoding (367/369 nt)	csrB	small regulatory RNA CsrB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (16/22);  total (16/22)

TGCGGCTGGGGTTCATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATT  >  NC_000913/2924019‑2924289                                                                                                                                             |                                                                                                                                    tGCGGCTGGGGTTCATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAt                                                                                                                                      <  2:787042/140‑1 (MQ=255)  gCGGCTGGGGTTCATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑a                                                                                                                                    >  2:635962/1‑140 (MQ=255)  gCGGCTGGGGTTCATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑a                                                                                                                                    >  1:71946/1‑140 (MQ=255)  gCGGCTGGGGTTCATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑a                                                                                                                                    >  2:763179/1‑140 (MQ=255)              cATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑aaaaa                                                                                                                                >  2:795700/1‑132 (MQ=255)              cATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑aaaaa                                                                                                                                <  1:795700/132‑1 (MQ=255)               aTCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGc                                                                                                                        <  2:150284/140‑1 (MQ=255)               aTCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGc                                                                                                                        >  2:243516/1‑140 (MQ=255)                tCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCa                                                                                                                       <  2:881856/140‑1 (MQ=255)                tCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCa                                                                                                                       <  2:597419/140‑1 (MQ=255)                 cGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCAc                                                                                                                      <  2:959595/140‑1 (MQ=255)                 cGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCAc                                                                                                                      <  2:720612/140‑1 (MQ=255)                 cGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCAc                                                                                                                      <  1:243516/140‑1 (MQ=255)                 cGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCAc                                                                                                                      <  1:1149473/140‑1 (MQ=255)                          aGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTcac                                                                                                             <  2:876232/140‑1 (MQ=255)                                        cGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTc                                                                                               >  2:758975/1‑140 (MQ=255)                                               aCGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCgc                                                                                        <  1:635962/140‑1 (MQ=255)                                                 gCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCgcag                                                                                      <  2:71946/140‑1 (MQ=255)                                                  cTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCgcagc                                                                                     <  2:867696/140‑1 (MQ=255)                                                    tCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCAt                                                                                   <  2:209404/140‑1 (MQ=255)                                                       aGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTcc                                                                                >  1:727221/1‑140 (MQ=255)                                                               cAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTAc                                                                          <  2:184369/138‑1 (MQ=255)                                                               cAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTAc                                                                          >  1:184369/1‑138 (MQ=255)                                                                       gaCGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCATTTCGCAGCATTCCAGCTACTTTTGTTGCt                                                                <  2:494513/140‑1 (MQ=255)                                                                             cgcgGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGc                                                          <  2:444255/140‑1 (MQ=255)                                                                                 gTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCAt                                                      >  1:718842/1‑140 (MQ=255)                                                                                     tGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCtt                                                  <  1:763179/140‑1 (MQ=255)                                                                                        gTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGAc                                               <  1:809007/140‑1 (MQ=255)                                                                                          ggTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACaa                                             >  2:533978/1‑140 (MQ=255)                                                                                            tCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACt                                           >  1:966193/1‑140 (MQ=255)                                                                                            tCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACt                                           >  1:77658/1‑140 (MQ=255)                                                                                            tCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACt                                           >  1:884122/1‑140 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTc                                       <  2:1118184/134‑1 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTc                                       >  1:1091239/1‑134 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTc                                       >  1:1118184/1‑134 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTc                                       <  2:1091239/134‑1 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTgg                                 >  2:656904/1‑140 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTgg                                 >  2:638199/1‑140 (MQ=255)                                                                                                      aCCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTgg                                 >  1:177027/1‑140 (MQ=255)                                                                                                               aGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGc                        <  1:547037/140‑1 (MQ=255)                                                                                                                     aCTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCg                  >  1:1044098/1‑140 (MQ=255)                                                                                                                      cTGCCGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGt                 <  2:1079452/140‑1 (MQ=255)                                                                                                                         ccGCGAAGGATAGCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCa              >  2:334644/1‑140 (MQ=255)                                                                                                                                     gCAGGAATAAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTAtt  <  2:718842/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                    TGCGGCTGGGGTTCATCGTTTCGCCAGTGCTGATGTTCACGCAGTAACGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAAT‑AAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATT  >  NC_000913/2924019‑2924289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑