Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,405,503	T→C	I23T (ATT→ACT)	accB →	biotin carboxyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,405,503	0	T	C	100.0%	267.9 / NA	74	I23T (ATT→ACT)	accB	biotin carboxyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/38);  total (36/38)

ATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCC  >  NC_000913/3405368‑3405638                                                                                                                                        |                                                                                                                                        aTACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCt                                                                                                                                     >  1:549015/1‑140 (MQ=255) aTACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCt                                                                                                                                     >  1:255144/1‑140 (MQ=255)         tACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGa                                                                                                                             <  1:691285/140‑1 (MQ=255)              aCGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGt                                                                                                                        <  1:1058373/140‑1 (MQ=255)                 gcggTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAg                                                                                                                     <  1:1036652/140‑1 (MQ=255)                 gcggTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAg                                                                                                                     >  1:528051/1‑140 (MQ=255)                   ggTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTa                                                                                                                   >  1:514699/1‑140 (MQ=255)                     tGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAagag                                                                                                                         <  2:64688/132‑1 (MQ=255)                     tGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAagag                                                                                                                         >  1:64688/1‑132 (MQ=255)                        aaCGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCAt                                                                                                              <  1:169161/140‑1 (MQ=255)                        aaCGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCAt                                                                                                              <  1:274244/140‑1 (MQ=255)                           gCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAg                                                                                                           <  1:748355/140‑1 (MQ=255)                           gCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAg                                                                                                           <  2:528051/140‑1 (MQ=255)                           gCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAg                                                                                                           <  2:514699/140‑1 (MQ=255)                            ccTGTCACAATCACACTAAACAAAGAGTACGGAACCCATTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGGAGGCGAAGAGTCAGTACGCATTAGc                                                                                                          >  1:979002/1‑140 (MQ=255)                            ccTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGc                                                                                                          >  1:290271/1‑140 (MQ=255)                            ccTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGc                                                                                                          >  2:143881/1‑140 (MQ=255)                               gTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGt                                                                                                       >  2:576557/1‑140 (MQ=255)                               gTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGt                                                                                                       >  1:876571/1‑140 (MQ=255)                                     aTCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCt                                                                                                 <  1:703417/140‑1 (MQ=255)                                            tAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCg                                                                                          >  2:435756/1‑140 (MQ=255)                                             aaaCAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGc                                                                                         <  1:943227/140‑1 (MQ=255)                                             aaaCAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGc                                                                                         >  1:696672/1‑140 (MQ=255)                                              aaCAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCa                                                                                        >  2:387725/1‑140 (MQ=255)                                                cAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAg                                                                                      >  1:1093560/1‑140 (MQ=255)                                                cAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAg                                                                                      >  2:1123139/1‑140 (MQ=255)                                                cAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAg                                                                                      <  1:648840/140‑1 (MQ=255)                                                cAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAg                                                                                      >  2:503014/1‑140 (MQ=255)                                                        aCGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCtg                                                                              >  1:1115030/1‑140 (MQ=255)                                                        aCGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCtg                                                                              <  2:763382/140‑1 (MQ=255)                                                        aCGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCtg                                                                              >  2:47997/1‑140 (MQ=255)                                                          ggAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCtgtg                                                                            <  1:576557/140‑1 (MQ=255)                                                          ggAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCtgtg                                                                            >  1:759229/1‑140 (MQ=255)                                                            aaCCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGAt                                                                          <  1:614611/140‑1 (MQ=255)                                                              cccACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGc                                                                        >  1:1130091/1‑140 (MQ=255)                                                              cccACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGc                                                                        <  1:581009/140‑1 (MQ=255)                                                               ccACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGca                                                                       >  2:191296/1‑140 (MQ=255)                                                               ccACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGc                                                                        >  2:1162802/1‑139 (MQ=255)                                                               ccACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGc                                                                        <  1:1162802/139‑1 (MQ=255)                                                                cACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGcaa                                                                      <  1:225499/140‑1 (MQ=255)                                                                cACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGcaa                                                                      <  1:224069/140‑1 (MQ=255)                                                                cACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGcaa                                                                      <  1:677121/140‑1 (MQ=255)                                                                cACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGcaa                                                                      <  1:47997/140‑1 (MQ=255)                                                                 aCTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGGAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGcaac                                                                     >  2:628988/1‑140 (MQ=255)                                                                 aCTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGcaac                                                                     >  1:1034856/1‑140 (MQ=255)                                                                      tGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCt                                                                >  1:1050563/1‑140 (MQ=255)                                                                        gATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTa                                                              <  1:1123139/140‑1 (MQ=255)                                                                        gATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTa                                                              <  2:882872/140‑1 (MQ=255)                                                                        gATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTa                                                              <  2:255144/140‑1 (MQ=255)                                                                           atTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGc                                                           <  1:435756/140‑1 (MQ=255)                                                                             tCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTg                                                         <  2:1130091/140‑1 (MQ=255)                                                                             tCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTg                                                         >  1:1135172/1‑140 (MQ=255)                                                                              cGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGc                                                        <  2:759229/140‑1 (MQ=255)                                                                              cGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGc                                                        >  2:678098/1‑140 (MQ=255)                                                                                 aaGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCAcc                                                     >  2:742886/1‑140 (MQ=255)                                                                                     ttAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAatg                                                 <  1:624810/140‑1 (MQ=255)                                                                                          aaaCTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATgca                                            <  1:678098/140‑1 (MQ=255)                                                                                              tGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGcagcag                                        >  2:270599/1‑140 (MQ=255)                                                                                               gATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCagcagc                                       <  1:387725/140‑1 (MQ=255)                                                                                               gATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCagcagc                                       >  2:492686/1‑140 (MQ=255)                                                                                                    aGCTGGTTGGAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCt                                  <  1:628988/140‑1 (MQ=255)                                                                                                    aGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCt                                  <  2:1115030/140‑1 (MQ=255)                                                                                                    aGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCt                                  <  2:771798/140‑1 (MQ=255)                                                                                                          ttGAAGAATCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCt                            >  2:44885/1‑140 (MQ=255)                                                                                                                 aTCAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAg                     <  2:1093560/140‑1 (MQ=255)                                                                                                                   cAGGCATCTCCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGcc                   >  2:7361/1‑140 (MQ=255)                                                                                                                           tcCGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGc           >  2:482167/1‑140 (MQ=255)                                                                                                                             cGAACTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGa         <  1:482167/140‑1 (MQ=255)                                                                                                                                aCTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCg      >  1:848261/1‑140 (MQ=255)                                                                                                                                 cTGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGt     <  2:876571/140‑1 (MQ=255)                                                                                                                                  tGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGTTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGtt    <  1:270599/140‑1 (MQ=255)                                                                                                                                  tGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGtt    <  2:1034856/140‑1 (MQ=255)                                                                                                                                  tGGAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGtt    <  1:143881/140‑1 (MQ=255)                                                                                                                                    gAAACTTCTGAAGGCGCAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGttcc  >  2:1014619/1‑140 (MQ=255)                                                                                                                                    gAAACTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGttcc  >  1:806223/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGCAGCTCCTGCCGCAAGTTTCCCTGTGATGCAACAAGCTTACGCTGCACCAATGATGCAGCAGCCAGCTCAATCTAACGCAGCCGCTCCGGCGACCGTTCC  >  NC_000913/3405368‑3405638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑