Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,523,234	A→G	T122A (ACC→GCC)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,523,234	0	A	G	100.0%	296.0 / NA	81	T122A (ACC→GCC)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/47);  total (34/47)

TGAAAGCCTTTATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGAT  >  NC_000913/3523106‑3523370                                                                                                                                 |                                                                                                                                         tGAAAGCCTTTATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTg                                                                                                                               <  1:855299/140‑1 (MQ=255)  gAAAGCCTTTATCGCGACAGAAGACAGCCGCTTTTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTgg                                                                                                                              <  1:499195/140‑1 (MQ=255)     aGCCTTTATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCga                                                                                                                           <  1:508651/140‑1 (MQ=255)       ccTTTATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCgaga                                                                                                                         >  1:200702/1‑140 (MQ=255)         tttATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTCGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGaaa                                                                                                                       >  2:987192/1‑140 (MQ=255)          ttATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAAc                                                                                                                      >  1:503192/1‑140 (MQ=255)                 gACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTcc                                                                                                               >  1:285386/1‑140 (MQ=255)                 gACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGAGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGCCACAAGATGCAAGTACCATTGCCCAGCAGCTGGCGAGAAActtcttac                                                                                                               >  1:359080/1‑138 (MQ=255)                  aCAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCt                                                                                                              <  2:467541/140‑1 (MQ=255)                  aCAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCt                                                                                                              <  2:1117165/140‑1 (MQ=255)                  aCAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCt                                                                                                              <  1:276043/140‑1 (MQ=255)                  aCAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCt                                                                                                              >  2:938248/1‑140 (MQ=255)                  aCAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCt                                                                                                              <  1:351901/140‑1 (MQ=255)                   cAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTc                                                                                                             <  2:503192/140‑1 (MQ=255)                      aagaCAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGt                                                                                                          >  1:261528/1‑140 (MQ=255)                       agaCAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTc                                                                                                         >  1:1133085/1‑140 (MQ=255)                       agaCAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTc                                                                                                         <  1:610006/140‑1 (MQ=255)                        gaCAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAA‑GGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCa                                                                                                       <  1:851973/140‑1 (MQ=255)                           aGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGa                                                                                                     <  1:834147/140‑1 (MQ=255)                           aGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGa                                                                                                     <  1:305945/140‑1 (MQ=255)                             ccGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTcc                                                                                                               <  1:476488/128‑1 (MQ=255)                             ccGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTcc                                                                                                               >  2:476488/1‑128 (MQ=255)                              cGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAacg                                                                                                  <  2:354695/140‑1 (MQ=255)                                cTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAacgca                                                                                                >  1:1057451/1‑140 (MQ=255)                                  tCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAacgcacg                                                                                              <  1:555427/140‑1 (MQ=255)                                     aCGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTg                                                                                           <  2:91492/140‑1 (MQ=255)                                     aCGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTg                                                                                           <  2:1032345/140‑1 (MQ=255)                                          cATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCg                                                                                      <  2:1066198/140‑1 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAacgcacgc                                                                                             <  1:446401/132‑1 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAacgcacgc                                                                                             >  2:446401/1‑132 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCt                                                                                            <  2:532957/133‑1 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCt                                                                                            >  1:532957/1‑133 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     <  2:360087/140‑1 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     >  2:1126214/1‑140 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     >  2:132950/1‑140 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     <  2:285386/140‑1 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     >  1:438839/1‑140 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     >  2:559052/1‑140 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     >  2:580211/1‑140 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     >  2:968925/1‑140 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     <  1:559052/140‑1 (MQ=255)                                           aTCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     <  1:573413/140‑1 (MQ=255)                                           aTCACGGCGTCGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGt                                                                                     <  1:987192/140‑1 (MQ=255)                                               cGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGa                                                                                 <  1:402868/140‑1 (MQ=255)                                               cGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGa                                                                                 <  1:938248/140‑1 (MQ=255)                                                ggCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGAt                                                                                <  2:438839/140‑1 (MQ=255)                                                  cGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTa                                                                              <  1:150801/140‑1 (MQ=255)                                                      gACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGa                                                                          <  1:561771/140‑1 (MQ=255)                                                       aCCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGaa                                                                         >  2:527722/1‑140 (MQ=255)                                                              ggggATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTcc                                                                  <  1:968925/140‑1 (MQ=255)                                                                     ttCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGTCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGAt                                                           <  2:1057451/140‑1 (MQ=255)                                                                     ttCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGAt                                                           <  1:476011/140‑1 (MQ=255)                                                                      tCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGAtt                                                          >  2:698399/1‑140 (MQ=255)                                                                       ccGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTc                                                         >  1:1070985/1‑140 (MQ=255)                                                                        cGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCg                                                        <  2:1070985/140‑1 (MQ=255)                                                                           gcagcaAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCAt                                                     >  2:668453/1‑140 (MQ=255)                                                                               caAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGaa                                                 >  1:440227/1‑140 (MQ=255)                                                                                   cGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAgc                                             <  1:384156/140‑1 (MQ=255)                                                                                    gTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCg                                                        >  1:471177/1‑128 (MQ=255)                                                                                    gTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCg                                                        <  2:471177/128‑1 (MQ=255)                                                                                           tGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACg                                     >  1:350321/1‑140 (MQ=255)                                                                                            gTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGa                                    <  1:527722/140‑1 (MQ=255)                                                                                                tcCGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGa                                <  2:200702/140‑1 (MQ=255)                                                                                                 ccGGTCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGAc                               >  2:941642/1‑140 (MQ=255)                                                                                                     tCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACgaga                           >  1:819863/1‑140 (MQ=255)                                                                                                     tCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACgaga                           <  1:941642/140‑1 (MQ=255)                                                                                                     tCACGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACgaga                           >  1:132531/1‑140 (MQ=255)                                                                                                       aCGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATc                         >  1:370758/1‑140 (MQ=255)                                                                                                       aCGCGTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATc                         >  1:311894/1‑140 (MQ=255)                                                                                                         gcgTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCt                       <  1:668453/140‑1 (MQ=255)                                                                                                         gcgTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCt                       <  2:350321/140‑1 (MQ=255)                                                                                                         gcgTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCt                       <  1:1126214/140‑1 (MQ=255)                                                                                                         gcgTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCt                       <  1:132950/140‑1 (MQ=255)                                                                                                         gcgTCACAAGGGGCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCt                       <  1:698399/140‑1 (MQ=255)                                                                                                                     gCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCt           >  2:116525/1‑140 (MQ=255)                                                                                                                     gCAAGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCt           >  1:982667/1‑140 (MQ=255)                                                                                                                       aaGTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGa         >  2:352328/1‑140 (MQ=255)                                                                                                                         gTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAAc       <  1:116525/140‑1 (MQ=255)                                                                                                                         gTACCATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAAc       <  2:440227/140‑1 (MQ=255)                                                                                                                            ccATTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAg    <  2:531546/140‑1 (MQ=255)                                                                                                                              aTTGCCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGAt  >  1:579304/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                         TGAAAGCCTTTATCGCGACAGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCAGCAAGCGTGGCGCTGTTCTCCGGTCACGCGTCACAAGGGGCAAGTACCATTACCCAGCAGCTGGCGAGAAACTTCTTCCTCAGTCCAGAACGCACGCTGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGCTGCTGACGAAAGACGAGATCCTCGAGCTTTATCTGAACAAGAT  >  NC_000913/3523106‑3523370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑