Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,647	T→C	E66E (GAA→GAG)	bioH ←	pimeloyl‑acyl carrier protein methyl ester esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,647	0	T	C	100.0%	241.9 / NA	67	E66E (GAA→GAG)	bioH	pimeloyl‑acyl carrier protein methyl ester esterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/29);  total (38/29)

AAGGTGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCAT  >  NC_000913/3544517‑3544782                                                                                                                                   |                                                                                                                                        aaGGTGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCata                                                                                                                                >  1:710851/1‑140 (MQ=255)   ggTGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATc                                                                                                                              >  1:673513/1‑140 (MQ=255)    gTGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCa                                                                                                                             <  2:327180/140‑1 (MQ=255)     tGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAg                                                                                                                            >  1:614621/1‑140 (MQ=255)     tGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAg                                                                                                                            >  2:572406/1‑140 (MQ=255)       acgacgCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCa                                                                                                                          >  1:116910/1‑140 (MQ=255)        cgacgCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCaa                                                                                                                         >  1:381694/1‑140 (MQ=255)              cACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGc                                                                                                                                    <  2:772938/123‑1 (MQ=255)              cACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGc                                                                                                                                    >  1:772938/1‑123 (MQ=255)               aCGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAg                                                                                                                  <  2:911942/140‑1 (MQ=255)                          cgcCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATc                                                                                                       >  2:896542/1‑140 (MQ=255)                                 acacGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCt                                                                                                <  2:895264/140‑1 (MQ=255)                                     gCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAACACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGc                                                                                            >  1:23127/1‑140 (MQ=255)                                      cTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGcc                                                                                           <  1:685529/140‑1 (MQ=255)                                       tCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGccc                                                                                          >  1:992156/1‑140 (MQ=255)                                          ggATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGaa                                                                                       >  2:745103/1‑140 (MQ=255)                                               ggTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCgg                                                                                  >  1:787452/1‑140 (MQ=255)                                                gTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                 <  2:332794/140‑1 (MQ=255)                                                gTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                 <  2:116910/140‑1 (MQ=255)                                                 ttAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGc                                                                                >  1:915607/1‑140 (MQ=255)                                                     cGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                 <  2:801318/135‑1 (MQ=255)                                                     cGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                 >  1:801318/1‑135 (MQ=255)                                                         aTCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaac                                                                        >  1:452099/1‑140 (MQ=255)                                                          tCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaaca                                                                       >  1:370812/1‑140 (MQ=255)                                                          tCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaaca                                                                       >  2:975571/1‑140 (MQ=255)                                                                ttGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGc                                                                 <  2:787452/140‑1 (MQ=255)                                                                  gCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAg                                                               <  1:896542/140‑1 (MQ=255)                                                                   ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGt                                                                            >  2:312995/1‑126 (MQ=255)                                                                   ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGt                                                                            <  1:312995/126‑1 (MQ=255)                                                                    caccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCg                                                             >  2:323304/1‑140 (MQ=255)                                                                    caccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCg                                                             >  1:101460/1‑140 (MQ=255)                                                                        aGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTaaa                                                         >  1:413801/1‑140 (MQ=255)                                                                              cccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGa                                                   >  1:351038/1‑140 (MQ=255)                                                                                  gACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTa                                               <  1:745103/140‑1 (MQ=255)                                                                                         gCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATg                                                      <  1:6927/126‑1 (MQ=255)                                                                                         gCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATg                                                      >  2:6927/1‑126 (MQ=255)                                                                                         gCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCt                                        >  2:495495/1‑140 (MQ=255)                                                                                         gCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCt                                        >  2:911368/1‑140 (MQ=255)                                                                                             aaCCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTc                                    >  1:357710/1‑140 (MQ=255)                                                                                             aaCCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTc                                    >  2:1000617/1‑140 (MQ=255)                                                                                              aCCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCa                                   >  1:116128/1‑140 (MQ=255)                                                                                               ccAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTc                                    <  2:414058/138‑1 (MQ=255)                                                                                               ccAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTc                                    >  1:414058/1‑138 (MQ=255)                                                                                               ccAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCaa                                  >  1:417752/1‑140 (MQ=255)                                                                                               ccAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCaa                                  >  2:629382/1‑140 (MQ=255)                                                                                               ccAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCaa                                  >  1:843375/1‑140 (MQ=255)                                                                                                 aaaTGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATg                                <  1:123012/140‑1 (MQ=255)                                                                                                 aaaTGGCTTTATCAGGTGCCTGTTGCAACACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATg                                <  2:23127/140‑1 (MQ=255)                                                                                                  aaTGGCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGc                               <  2:370812/140‑1 (MQ=255)                                                                                                      gCTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACg                           >  1:764933/1‑140 (MQ=255)                                                                                                       cTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGc                          <  2:351038/140‑1 (MQ=255)                                                                                                       cTTTATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGc                          <  2:413801/140‑1 (MQ=255)                                                                                                         ttATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCca                        <  2:273021/140‑1 (MQ=255)                                                                                                          tATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCcac                       >  2:534725/1‑140 (MQ=255)                                                                                                          tATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCcac                       >  1:793104/1‑140 (MQ=255)                                                                                                          tATCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCcac                       >  1:1057831/1‑140 (MQ=255)                                                                                                           aTCAGGTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCcaca                      <  2:793104/140‑1 (MQ=255)                                                                                                               ggTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTc                  <  1:806798/140‑1 (MQ=255)                                                                                                                gTGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCg                 <  1:602994/140‑1 (MQ=255)                                                                                                                 tGCCTGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCgg                >  1:393950/1‑140 (MQ=255)                                                                                                                     tGTTGCAGCTCGGCGTCGGCCATATCAGCAAGTGACAGCGCCCCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCGTCGTCAATGCAACGCCACACTTCGGCAtt            <  2:665357/140‑1 (MQ=255)                                                                                                                     tGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCAtt            <  1:911368/140‑1 (MQ=255)                                                                                                                     tGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCAtt            <  2:1163016/140‑1 (MQ=255)                                                                                                                     tGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCAtt            <  1:1000617/140‑1 (MQ=255)                                                                                                                     tGTTGCAGCACGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCAtt            <  1:629382/140‑1 (MQ=255)                                                                                                                      gTTGCAGCACGGCCTTGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTc           >  1:16170/1‑140 (MQ=255)                                                                                                                          cagcaCGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTc       <  2:992156/140‑1 (MQ=255)                                                                                                                               cGGCCTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCAt  <  2:393950/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        AAGGTGACGACGCCACGGTGACCAGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCAT  >  NC_000913/3544517‑3544782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑