Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,598,987	A→G	G224G (GGT→GGC)	livJ ←	branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,598,987	0	A	G	100.0%	261.2 / NA	72	G224G (GGT→GGC)	livJ	branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/33);  total (39/33)

TCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCT  >  NC_000913/3598851‑3599122                                                                                                                                         |                                                                                                                                        tCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGccg                                                                                                                                      <  2:37020/140‑1 (MQ=255) tCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTCCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGccg                                                                                                                                      <  2:824382/140‑1 (MQ=255)  cACCAGCAGCCCTTCCGCTGATTCGCCTGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGccgc                                                                                                                                     <  1:291314/140‑1 (MQ=255)      agcagcCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCgta                                                                                                                                 >  2:432373/1‑140 (MQ=255)        cagcCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAACCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAgccgcc                                                                                                                                    >  1:434198/1‑135 (MQ=255)        cagcCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAACCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAgccgcc                                                                                                                                    <  2:434198/135‑1 (MQ=255)          gcCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTaa                                                                                                                             <  2:532895/140‑1 (MQ=255)          gcCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTaa                                                                                                                             >  1:100095/1‑140 (MQ=255)           cccTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTaaa                                                                                                                            >  2:345056/1‑140 (MQ=255)             cTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACg                                                                                                                          >  1:1066713/1‑140 (MQ=255)                       ttCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGatat                                                                                                                <  1:721812/140‑1 (MQ=255)                        tCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATAtt                                                                                                               >  2:446574/1‑140 (MQ=255)                          gCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATtct                                                                                                             <  1:388858/140‑1 (MQ=255)                                aaTGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCttttt                                                                                                       >  2:810334/1‑140 (MQ=255)                                 aTGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCtttttt                                                                                                      <  1:432373/140‑1 (MQ=255)                                     tAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGa                                                                                                  <  2:944343/140‑1 (MQ=255)                                     tAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGa                                                                                                  <  2:195394/140‑1 (MQ=255)                                     tAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGa                                                                                                  <  1:446574/140‑1 (MQ=255)                                      aGACAGCGAAACGTTAGCCACACCTTCCGG‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATtct                                                                                                             >  1:1098739/1‑127 (MQ=255)                                      aGACAGCGAAACGTTAGCCACACCTTCCGG‑CCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATtct                                                                                                             <  2:1098739/127‑1 (MQ=255)                                       gACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGAcg                                                                                                <  1:810334/140‑1 (MQ=255)                                            cGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGcca                                                                                           >  1:981215/1‑140 (MQ=255)                                            cGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGcca                                                                                           >  2:529300/1‑140 (MQ=255)                                            cGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGcca                                                                                           >  2:885836/1‑140 (MQ=255)                                            cGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGcca                                                                                           >  1:1033087/1‑140 (MQ=255)                                            cGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGcca                                                                                           >  2:902807/1‑140 (MQ=255)                                            cGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTAGATATTCTCTTTTTTCAGACGCGcca                                                                                           >  2:1032121/1‑140 (MQ=255)                                             gAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGAcgc                                                                                               <  1:763713/135‑1 (MQ=255)                                             gAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGAcgc                                                                                               >  2:763713/1‑135 (MQ=255)                                               aaCGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGccacca                                                                                        <  2:708821/140‑1 (MQ=255)                                                 cGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCGGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGc                                                                                      <  1:560158/140‑1 (MQ=255)                                                           acCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAAt                                                                            >  1:639348/1‑140 (MQ=255)                                                           acCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAAt                                                                            >  1:1131200/1‑140 (MQ=255)                                                           acCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAAt                                                                            >  2:544193/1‑140 (MQ=255)                                                           acCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAAt                                                                            >  1:480679/1‑140 (MQ=255)                                                               tCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCttt                                                                        >  1:379788/1‑140 (MQ=255)                                                                  ggCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCCTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTc                                                                     >  1:380306/1‑140 (MQ=255)                                                                    ccccATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTccc                                                                   >  1:642958/1‑140 (MQ=255)                                                                         tAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCcggcg                                                              >  2:611027/1‑140 (MQ=255)                                                                         tAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCcggcg                                                              >  2:33579/1‑140 (MQ=255)                                                                           aaCTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGt                                                            <  1:902807/140‑1 (MQ=255)                                                                              tGAGTTTACAGCCCCGCCGCGGGTGCATGACGCGGGATTTGCCCCATTTACGGCGGATGGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGAt                                                         <  2:78686/140‑1 (MQ=25)                                                                               gAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATg                                                        <  1:834566/140‑1 (MQ=255)                                                                               gAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATg                                                        <  1:1032121/140‑1 (MQ=255)                                                                               gAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCCTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATg                                                        <  2:380306/140‑1 (MQ=255)                                                                                aGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGc                                                       >  2:911382/1‑140 (MQ=255)                                                                                aGTTTCCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGc                                                       >  2:886922/1‑140 (MQ=255)                                                                                  ttttCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCa                                                     <  1:164213/140‑1 (MQ=255)                                                                                  ttttCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCa                                                     >  1:685161/1‑140 (MQ=255)                                                                                     tCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCa                                                  <  2:685161/140‑1 (MQ=255)                                                                                     tCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCa                                                  >  1:526381/1‑140 (MQ=255)                                                                                     ccAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCa                                                  <  1:886922/139‑1 (MQ=255)                                                                                        gCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAg                                               <  1:345056/140‑1 (MQ=255)                                                                                            tGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTGAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAaca                                           >  2:1020357/1‑140 (MQ=255)                                                                                              ccGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCa                                                     <  2:346727/128‑1 (MQ=255)                                                                                              ccGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCa                                                     >  1:346727/1‑128 (MQ=255)                                                                                              ccGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAac                                            <  2:515786/137‑1 (MQ=255)                                                                                              ccGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAac                                            >  1:515786/1‑137 (MQ=255)                                                                                                     tGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTg                                  <  2:480679/140‑1 (MQ=255)                                                                                                       ccTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCa                                >  2:75462/1‑140 (MQ=255)                                                                                                        cTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAacac                                          >  1:593845/1‑129 (MQ=255)                                                                                                        cTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAacac                                          <  2:593845/129‑1 (MQ=255)                                                                                                        cTGACGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCAt                               <  1:75462/140‑1 (MQ=255)                                                                                                            cGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACg                                      >  1:58284/1‑129 (MQ=255)                                                                                                            cGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACg                                      <  2:58284/129‑1 (MQ=255)                                                                                                            cGCAGGATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGcc                           >  1:428858/1‑140 (MQ=255)                                                                                                                ggATTTGCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTttc                       >  2:260654/1‑140 (MQ=255)                                                                                                                      gCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                 <  2:1066713/140‑1 (MQ=255)                                                                                                                      gCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                 <  2:526381/140‑1 (MQ=255)                                                                                                                      gCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                 <  1:911382/140‑1 (MQ=255)                                                                                                                      gCCCCATTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAgg                 >  1:715752/1‑140 (MQ=255)                                                                                                                           aTTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATGTTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATTTCAACAAACAACACCAGGTTTGCATTGTCCTTCTTCAGGCCGTc            >  2:644801/1‑140 (MQ=25)                                                                                                                           aTTTCCGGGTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTc            >  2:1121695/1‑140 (MQ=255)                                                                                                                                  ggTGATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCAcc     >  1:499245/1‑140 (MQ=255)                                                                                                                                     gATAGCCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCt  <  2:981215/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCACCAGCAGCCCTTCCGCTGATTCGCCCGCAATGTTAGACAGCGAAACGTTAGCCACACCTTCCGGCCCCATAAACTGAGTTTTCAGCCCTGCCGCGCGTGCCTGACGCAGGATTTGCCCCATTTCCGGGTGATAACCGCCGTAGTAAACGAAGTCGATATTCTCTTTTTTCAGACGCGCCACCAGCGTTGAGAAATCTTTTTCCCCGGCGGTGATGCCATCAAAGAACACCACGTTTGCATTGCCTTTCTTCAGGCCGTCCTGCACCGCT  >  NC_000913/3598851‑3599122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑